Spontaneous Aberrant Bodies Formation in Human Pneumocytes Infected with <i>Estrella lausannensis</i>
<i>Estrella lausannensis</i>, a <i>Chlamydia</i>-related bacterium isolated from a Spanish river, is considered as a possible emerging human pathogen. Indeed, it was recently demonstrated to multiply in human macrophages, resisting oxidative burst and causing a strong cytopat...
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| Format: | Article |
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MDPI AG
2023-09-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/10/2368 |
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| author | Aurelien Rovero Carole Kebbi-Beghdadi Gilbert Greub |
| author_facet | Aurelien Rovero Carole Kebbi-Beghdadi Gilbert Greub |
| author_sort | Aurelien Rovero |
| collection | DOAJ |
| description | <i>Estrella lausannensis</i>, a <i>Chlamydia</i>-related bacterium isolated from a Spanish river, is considered as a possible emerging human pathogen. Indeed, it was recently demonstrated to multiply in human macrophages, resisting oxidative burst and causing a strong cytopathic effect. In addition, a preliminary study highlighted a correlation between antibody response to <i>E. lausannensis</i> and pneumonia in children. To clarify the pathogenic potential of these bacteria, we infected a human pneumocyte cell line with <i>E. lausannensis</i> and assessed its replication and cytopathic effect using quantitative real-time PCR and immunofluorescence, as well as confocal and electron microscopy. Our results demonstrated that <i>E. lausannensis</i> enters and replicates rapidly in human pneumocytes, and that it causes a prompt lysis of the host cells. Furthermore, we reported the spontaneous formation of aberrant bodies, a form associated with persistence in <i>Chlamydiae</i>, suggesting that <i>E. lausannensis</i> infection could cause chronic disorders in humans. |
| first_indexed | 2024-03-10T21:02:37Z |
| format | Article |
| id | doaj.art-781577dc670f4061b2243de29d561623 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T21:02:37Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-781577dc670f4061b2243de29d5616232023-11-19T17:25:39ZengMDPI AGMicroorganisms2076-26072023-09-011110236810.3390/microorganisms11102368Spontaneous Aberrant Bodies Formation in Human Pneumocytes Infected with <i>Estrella lausannensis</i>Aurelien Rovero0Carole Kebbi-Beghdadi1Gilbert Greub2Institute of Microbiology, Lausanne University Hospital and University of Lausanne, 1011 Lausanne, SwitzerlandInstitute of Microbiology, Lausanne University Hospital and University of Lausanne, 1011 Lausanne, SwitzerlandInstitute of Microbiology, Lausanne University Hospital and University of Lausanne, 1011 Lausanne, Switzerland<i>Estrella lausannensis</i>, a <i>Chlamydia</i>-related bacterium isolated from a Spanish river, is considered as a possible emerging human pathogen. Indeed, it was recently demonstrated to multiply in human macrophages, resisting oxidative burst and causing a strong cytopathic effect. In addition, a preliminary study highlighted a correlation between antibody response to <i>E. lausannensis</i> and pneumonia in children. To clarify the pathogenic potential of these bacteria, we infected a human pneumocyte cell line with <i>E. lausannensis</i> and assessed its replication and cytopathic effect using quantitative real-time PCR and immunofluorescence, as well as confocal and electron microscopy. Our results demonstrated that <i>E. lausannensis</i> enters and replicates rapidly in human pneumocytes, and that it causes a prompt lysis of the host cells. Furthermore, we reported the spontaneous formation of aberrant bodies, a form associated with persistence in <i>Chlamydiae</i>, suggesting that <i>E. lausannensis</i> infection could cause chronic disorders in humans.https://www.mdpi.com/2076-2607/11/10/2368<i>Chlamydia</i>-related bacteriumreplicationpersistencechronic infectioncytopathic effect |
| spellingShingle | Aurelien Rovero Carole Kebbi-Beghdadi Gilbert Greub Spontaneous Aberrant Bodies Formation in Human Pneumocytes Infected with <i>Estrella lausannensis</i> Microorganisms <i>Chlamydia</i>-related bacterium replication persistence chronic infection cytopathic effect |
| title | Spontaneous Aberrant Bodies Formation in Human Pneumocytes Infected with <i>Estrella lausannensis</i> |
| title_full | Spontaneous Aberrant Bodies Formation in Human Pneumocytes Infected with <i>Estrella lausannensis</i> |
| title_fullStr | Spontaneous Aberrant Bodies Formation in Human Pneumocytes Infected with <i>Estrella lausannensis</i> |
| title_full_unstemmed | Spontaneous Aberrant Bodies Formation in Human Pneumocytes Infected with <i>Estrella lausannensis</i> |
| title_short | Spontaneous Aberrant Bodies Formation in Human Pneumocytes Infected with <i>Estrella lausannensis</i> |
| title_sort | spontaneous aberrant bodies formation in human pneumocytes infected with i estrella lausannensis i |
| topic | <i>Chlamydia</i>-related bacterium replication persistence chronic infection cytopathic effect |
| url | https://www.mdpi.com/2076-2607/11/10/2368 |
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