Transfection Agent Induced Nanoparticle Cell Loading
Loading cells with magnetic nanoparticles, and tracking their fate in vivo by high resolution MRI, is an attractive approach for enhancing the efficacy of cell-based therapies including those utilizing hematopoietic stem cells, neuroprogenitor cells, and T cells. The transfection agent (internalizat...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publications
2005-07-01
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| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.1162/15353500200505100 |
| _version_ | 1797283939285467136 |
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| author | Karin Montet-Abou Xavier Montet Ralph Weissleder Lee Josephson |
| author_facet | Karin Montet-Abou Xavier Montet Ralph Weissleder Lee Josephson |
| author_sort | Karin Montet-Abou |
| collection | DOAJ |
| description | Loading cells with magnetic nanoparticles, and tracking their fate in vivo by high resolution MRI, is an attractive approach for enhancing the efficacy of cell-based therapies including those utilizing hematopoietic stem cells, neuroprogenitor cells, and T cells. The transfection agent (internalization agent) assisted loading with the Feridex IV ® nanoparticle is an attractive method of loading because of the low cost of materials, and possible low regulatory barriers for eventual clinical use. We therefore explored the interaction between Feridex IV ® and three internalization agents protamine (PRO), polylysine (PLL), and lipofectamine (LFA). Feridex reacted with internalization agents to form aggregates, except when either the internalization agent or Feridex was present in large excess. When Jurkat T cells were incubated with Feridex/LFA or Feridex/PRO mixtures, and washed by centrifugation, nanoparticle aggregates co-purified with cells. With C17.2 cells large iron oxide particles adhered to the cell surface. At 30 μg/mL Feridex and 3 μg/mL LFA, internalization was largely mediated by LFA and was largely cytoplasmic. However, we found that the conditions used to label cells with Feridex and transfection agents need to be carefully selected to avoid the problems of surface adsorption and nanoparticle precipitation. |
| first_indexed | 2024-03-07T17:39:03Z |
| format | Article |
| id | doaj.art-781897217353421ea004babe33e5f175 |
| institution | Directory Open Access Journal |
| issn | 1536-0121 |
| language | English |
| last_indexed | 2024-03-07T17:39:03Z |
| publishDate | 2005-07-01 |
| publisher | SAGE Publications |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj.art-781897217353421ea004babe33e5f1752024-03-02T16:18:58ZengSAGE PublicationsMolecular Imaging1536-01212005-07-01410.1162/1535350020050510010.1162_15353500200505100Transfection Agent Induced Nanoparticle Cell LoadingKarin Montet-AbouXavier MontetRalph WeisslederLee JosephsonLoading cells with magnetic nanoparticles, and tracking their fate in vivo by high resolution MRI, is an attractive approach for enhancing the efficacy of cell-based therapies including those utilizing hematopoietic stem cells, neuroprogenitor cells, and T cells. The transfection agent (internalization agent) assisted loading with the Feridex IV ® nanoparticle is an attractive method of loading because of the low cost of materials, and possible low regulatory barriers for eventual clinical use. We therefore explored the interaction between Feridex IV ® and three internalization agents protamine (PRO), polylysine (PLL), and lipofectamine (LFA). Feridex reacted with internalization agents to form aggregates, except when either the internalization agent or Feridex was present in large excess. When Jurkat T cells were incubated with Feridex/LFA or Feridex/PRO mixtures, and washed by centrifugation, nanoparticle aggregates co-purified with cells. With C17.2 cells large iron oxide particles adhered to the cell surface. At 30 μg/mL Feridex and 3 μg/mL LFA, internalization was largely mediated by LFA and was largely cytoplasmic. However, we found that the conditions used to label cells with Feridex and transfection agents need to be carefully selected to avoid the problems of surface adsorption and nanoparticle precipitation.https://doi.org/10.1162/15353500200505100 |
| spellingShingle | Karin Montet-Abou Xavier Montet Ralph Weissleder Lee Josephson Transfection Agent Induced Nanoparticle Cell Loading Molecular Imaging |
| title | Transfection Agent Induced Nanoparticle Cell Loading |
| title_full | Transfection Agent Induced Nanoparticle Cell Loading |
| title_fullStr | Transfection Agent Induced Nanoparticle Cell Loading |
| title_full_unstemmed | Transfection Agent Induced Nanoparticle Cell Loading |
| title_short | Transfection Agent Induced Nanoparticle Cell Loading |
| title_sort | transfection agent induced nanoparticle cell loading |
| url | https://doi.org/10.1162/15353500200505100 |
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