Quorum Sensing Signals Alter in vitro Soil Virus Abundance and Bacterial Community Composition
Cell-density dependent quorum sensing (QS) is fundamental for many coordinated behaviors among bacteria. Most recently several studies have revealed a role for bacterial QS communication in bacteriophage (phage) reproductive decisions. However, QS based phage-host interactions remain largely unknown...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2020-06-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2020.01287/full |
| _version_ | 1819011255371825152 |
|---|---|
| author | Xiaolong Liang Regan E. Wagner Bingxue Li Ning Zhang Mark Radosevich |
| author_facet | Xiaolong Liang Regan E. Wagner Bingxue Li Ning Zhang Mark Radosevich |
| author_sort | Xiaolong Liang |
| collection | DOAJ |
| description | Cell-density dependent quorum sensing (QS) is fundamental for many coordinated behaviors among bacteria. Most recently several studies have revealed a role for bacterial QS communication in bacteriophage (phage) reproductive decisions. However, QS based phage-host interactions remain largely unknown, with the mechanistic details revealed for only a few phage-host pairs and a dearth of information available at the microbial community level. Here we report on the specific action of eight different individual QS signals (acyl-homoserine lactones; AHLs varying in acyl-chain length from four to 14 carbon atoms) on prophage induction in soil microbial communities. We show QS autoinducers, triggered prophage induction in soil bacteria and the response was significant enough to alter bacterial community composition in vitro. AHL treatment significantly decreased the bacterial diversity (Shannon Index) but did not significantly impact species richness. Exposure to short chain-length AHLs resulted in a decrease in the abundance of different taxa than exposure to higher molecular weight AHLs. Each AHL targeted a different subset of bacterial taxa. Our observations indicate that individual AHLs may trigger prophage induction in different bacterial taxa leading to changes in microbial community structure. The findings also have implications for the role of phage-host interactions in ecologically significant processes such as biogeochemical cycles, and phage mediated transfer of host genes, e.g., photosynthesis and heavy metal/antibiotic resistance. |
| first_indexed | 2024-12-21T01:25:15Z |
| format | Article |
| id | doaj.art-7818c3e4bc7a40128a72598b2e14b463 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-21T01:25:15Z |
| publishDate | 2020-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-7818c3e4bc7a40128a72598b2e14b4632022-12-21T19:20:32ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-06-011110.3389/fmicb.2020.01287532334Quorum Sensing Signals Alter in vitro Soil Virus Abundance and Bacterial Community CompositionXiaolong Liang0Regan E. Wagner1Bingxue Li2Ning Zhang3Mark Radosevich4Department of Biosystems Engineering and Soil Science, The University of Tennessee, Knoxville, Knoxville, TN, United StatesDepartment of Biosystems Engineering and Soil Science, The University of Tennessee, Knoxville, Knoxville, TN, United StatesCollege of Land and Environment, Shenyang Agricultural University, Shenyang, ChinaCollege of Biotechnology, Shenyang Agricultural University, Shenyang, ChinaDepartment of Biosystems Engineering and Soil Science, The University of Tennessee, Knoxville, Knoxville, TN, United StatesCell-density dependent quorum sensing (QS) is fundamental for many coordinated behaviors among bacteria. Most recently several studies have revealed a role for bacterial QS communication in bacteriophage (phage) reproductive decisions. However, QS based phage-host interactions remain largely unknown, with the mechanistic details revealed for only a few phage-host pairs and a dearth of information available at the microbial community level. Here we report on the specific action of eight different individual QS signals (acyl-homoserine lactones; AHLs varying in acyl-chain length from four to 14 carbon atoms) on prophage induction in soil microbial communities. We show QS autoinducers, triggered prophage induction in soil bacteria and the response was significant enough to alter bacterial community composition in vitro. AHL treatment significantly decreased the bacterial diversity (Shannon Index) but did not significantly impact species richness. Exposure to short chain-length AHLs resulted in a decrease in the abundance of different taxa than exposure to higher molecular weight AHLs. Each AHL targeted a different subset of bacterial taxa. Our observations indicate that individual AHLs may trigger prophage induction in different bacterial taxa leading to changes in microbial community structure. The findings also have implications for the role of phage-host interactions in ecologically significant processes such as biogeochemical cycles, and phage mediated transfer of host genes, e.g., photosynthesis and heavy metal/antibiotic resistance.https://www.frontiersin.org/article/10.3389/fmicb.2020.01287/fullsoilprophageinductioncommunitydiversityquorum sensing |
| spellingShingle | Xiaolong Liang Regan E. Wagner Bingxue Li Ning Zhang Mark Radosevich Quorum Sensing Signals Alter in vitro Soil Virus Abundance and Bacterial Community Composition Frontiers in Microbiology soil prophage induction community diversity quorum sensing |
| title | Quorum Sensing Signals Alter in vitro Soil Virus Abundance and Bacterial Community Composition |
| title_full | Quorum Sensing Signals Alter in vitro Soil Virus Abundance and Bacterial Community Composition |
| title_fullStr | Quorum Sensing Signals Alter in vitro Soil Virus Abundance and Bacterial Community Composition |
| title_full_unstemmed | Quorum Sensing Signals Alter in vitro Soil Virus Abundance and Bacterial Community Composition |
| title_short | Quorum Sensing Signals Alter in vitro Soil Virus Abundance and Bacterial Community Composition |
| title_sort | quorum sensing signals alter in vitro soil virus abundance and bacterial community composition |
| topic | soil prophage induction community diversity quorum sensing |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2020.01287/full |
| work_keys_str_mv | AT xiaolongliang quorumsensingsignalsalterinvitrosoilvirusabundanceandbacterialcommunitycomposition AT reganewagner quorumsensingsignalsalterinvitrosoilvirusabundanceandbacterialcommunitycomposition AT bingxueli quorumsensingsignalsalterinvitrosoilvirusabundanceandbacterialcommunitycomposition AT ningzhang quorumsensingsignalsalterinvitrosoilvirusabundanceandbacterialcommunitycomposition AT markradosevich quorumsensingsignalsalterinvitrosoilvirusabundanceandbacterialcommunitycomposition |