PsnWRKY70 Negatively Regulates NaHCO<sub>3</sub> Tolerance in <i>Populus</i>
Poplar is an important afforestation and ornamental tree species in Northeast China. The distribution area of saline-alkali land is approximately 765 hm<sup>2</sup> in Northeast China. The breeding of saline-alkali-resistant transgenic trees could be an effective method of afforestation...
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MDPI AG
2022-10-01
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| Series: | International Journal of Molecular Sciences |
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| author | Wei Wang Xiang-Dong Bai Kun Chen Xiao-Yue Zhang Chen-Rui Gu Jing Jiang Chuan-Ping Yang Gui-Feng Liu |
| author_facet | Wei Wang Xiang-Dong Bai Kun Chen Xiao-Yue Zhang Chen-Rui Gu Jing Jiang Chuan-Ping Yang Gui-Feng Liu |
| author_sort | Wei Wang |
| collection | DOAJ |
| description | Poplar is an important afforestation and ornamental tree species in Northeast China. The distribution area of saline-alkali land is approximately 765 hm<sup>2</sup> in Northeast China. The breeding of saline-alkali-resistant transgenic trees could be an effective method of afforestation in saline-alkali land. WRKY transcription factors play a crucial role in abiotic stress. In this study, we analyzed the genetic stability of the two-year-old <i>PsnWRKY70</i> transgenic poplars. The results showed that <i>PsnWRKY70</i> of transgenic poplars had been expressed stably and normally at the mRNA level. The gene interference expression (RE) lines had no significant effect on the growth of <i>PsnWRKY70</i> under NaHCO<sub>3</sub> stress, and the alkali damage index of RE lines was significantly lower than that of WT and overexpression (OE) lines at day 15 under NaHCO<sub>3</sub> stress. POD activity was significantly higher in RE lines than in WT. The MDA content of the RE line was lower than that of the WT line. Transcriptome analysis showed that RE lines up-regulated genes enriched in cell wall organization or biogenesis pathway-related genes such as <i>EXPA8</i>, <i>EXPA4</i>, <i>EXPA3</i>, <i>EXPA1</i>, <i>EXPB3</i>, <i>EXP10</i>, <i>PME53</i>, <i>PME34</i>, <i>PME36</i>, <i>XTH9</i>, <i>XTH6</i>, <i>XTH23</i>, <i>CESA1</i>, <i>CESA3</i>, <i>CES9</i>; <i>FLA11</i>, <i>FLA16</i> and <i>FLA7</i> genes. These genes play an important role in NaHCO<sub>3</sub> stress. Our study showed that the interference expression of the <i>PsnWRKY70</i> gene can enhance the tolerance of NaHCO<sub>3</sub> in poplar. |
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| issn | 1661-6596 1422-0067 |
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| spelling | doaj.art-781b2f20114641edad4195bd2f19dd5f2023-11-24T05:02:18ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-10-0123211308610.3390/ijms232113086PsnWRKY70 Negatively Regulates NaHCO<sub>3</sub> Tolerance in <i>Populus</i>Wei Wang0Xiang-Dong Bai1Kun Chen2Xiao-Yue Zhang3Chen-Rui Gu4Jing Jiang5Chuan-Ping Yang6Gui-Feng Liu7State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 26 Hexing Road, Harbin 150040, ChinaState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 26 Hexing Road, Harbin 150040, ChinaState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 26 Hexing Road, Harbin 150040, ChinaState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 26 Hexing Road, Harbin 150040, ChinaState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 26 Hexing Road, Harbin 150040, ChinaState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 26 Hexing Road, Harbin 150040, ChinaState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 26 Hexing Road, Harbin 150040, ChinaState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 26 Hexing Road, Harbin 150040, ChinaPoplar is an important afforestation and ornamental tree species in Northeast China. The distribution area of saline-alkali land is approximately 765 hm<sup>2</sup> in Northeast China. The breeding of saline-alkali-resistant transgenic trees could be an effective method of afforestation in saline-alkali land. WRKY transcription factors play a crucial role in abiotic stress. In this study, we analyzed the genetic stability of the two-year-old <i>PsnWRKY70</i> transgenic poplars. The results showed that <i>PsnWRKY70</i> of transgenic poplars had been expressed stably and normally at the mRNA level. The gene interference expression (RE) lines had no significant effect on the growth of <i>PsnWRKY70</i> under NaHCO<sub>3</sub> stress, and the alkali damage index of RE lines was significantly lower than that of WT and overexpression (OE) lines at day 15 under NaHCO<sub>3</sub> stress. POD activity was significantly higher in RE lines than in WT. The MDA content of the RE line was lower than that of the WT line. Transcriptome analysis showed that RE lines up-regulated genes enriched in cell wall organization or biogenesis pathway-related genes such as <i>EXPA8</i>, <i>EXPA4</i>, <i>EXPA3</i>, <i>EXPA1</i>, <i>EXPB3</i>, <i>EXP10</i>, <i>PME53</i>, <i>PME34</i>, <i>PME36</i>, <i>XTH9</i>, <i>XTH6</i>, <i>XTH23</i>, <i>CESA1</i>, <i>CESA3</i>, <i>CES9</i>; <i>FLA11</i>, <i>FLA16</i> and <i>FLA7</i> genes. These genes play an important role in NaHCO<sub>3</sub> stress. Our study showed that the interference expression of the <i>PsnWRKY70</i> gene can enhance the tolerance of NaHCO<sub>3</sub> in poplar.https://www.mdpi.com/1422-0067/23/21/13086poplar<i>PsnWRKY70</i>transcription factorNaHCO<sub>3</sub> stressgene expression |
| spellingShingle | Wei Wang Xiang-Dong Bai Kun Chen Xiao-Yue Zhang Chen-Rui Gu Jing Jiang Chuan-Ping Yang Gui-Feng Liu PsnWRKY70 Negatively Regulates NaHCO<sub>3</sub> Tolerance in <i>Populus</i> International Journal of Molecular Sciences poplar <i>PsnWRKY70</i> transcription factor NaHCO<sub>3</sub> stress gene expression |
| title | PsnWRKY70 Negatively Regulates NaHCO<sub>3</sub> Tolerance in <i>Populus</i> |
| title_full | PsnWRKY70 Negatively Regulates NaHCO<sub>3</sub> Tolerance in <i>Populus</i> |
| title_fullStr | PsnWRKY70 Negatively Regulates NaHCO<sub>3</sub> Tolerance in <i>Populus</i> |
| title_full_unstemmed | PsnWRKY70 Negatively Regulates NaHCO<sub>3</sub> Tolerance in <i>Populus</i> |
| title_short | PsnWRKY70 Negatively Regulates NaHCO<sub>3</sub> Tolerance in <i>Populus</i> |
| title_sort | psnwrky70 negatively regulates nahco sub 3 sub tolerance in i populus i |
| topic | poplar <i>PsnWRKY70</i> transcription factor NaHCO<sub>3</sub> stress gene expression |
| url | https://www.mdpi.com/1422-0067/23/21/13086 |
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