Comparison of Different Methods for Carotenoid Extraction from Dunaliella Salina
Background: Dunaliella salina (D. salina) is a unicellular marine alga known for its carotenoid ingredient. Carotenoid has been used in food industry as a coloring additive and as an antioxidant. In addition, it has anticancer, antiaging, and immune-modulatory properties. Different methods have been...
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| Format: | Article |
| Language: | English |
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Shiraz University of Medical Sciences
2021-12-01
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| Series: | International Journal of Nutrition Sciences |
| Subjects: | |
| Online Access: | https://ijns.sums.ac.ir/article_48048_8fe8c0e7baf3a1026ca814b3a9ffe4e4.pdf |
| _version_ | 1819261379923673088 |
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| author | Maryam Nejadmansouri Mohammad-Taghi Golmakani Mandana Famouri |
| author_facet | Maryam Nejadmansouri Mohammad-Taghi Golmakani Mandana Famouri |
| author_sort | Maryam Nejadmansouri |
| collection | DOAJ |
| description | Background: Dunaliella salina (D. salina) is a unicellular marine alga known for its carotenoid ingredient. Carotenoid has been used in food industry as a coloring additive and as an antioxidant. In addition, it has anticancer, antiaging, and immune-modulatory properties. Different methods have been used for extraction of carotenoids from algae. This study evaluated different extraction methods for carotenoid and comparedthe carotenoid yield and extraction time for procedures.Methods: In an experimental study, D. salina was isolated from Maharloo Lake in Shiraz, Iran. D. salina was centrifuged, dried, and suspended in methanol for further carotenoid extraction using different methods, namely ultrasound-assisted, supercritical fluid, microwave-assisted, electromantle, magnetic stirring, and maceration extraction methods.Results: Simultaneously, ultrasound-assisted, microwave-assisted, and electromantle extraction methods showed higher carotenoid yield and during a shorter time when compared with supercritical fluid, magnetic stirring, and maceration extraction methods. Since ultrasound-assisted extraction is a cold extraction method and ultrasound waves could release carotenoid and chlorophyll from broken cells to solvent quickly, the highest yields of carotenoid and chlorophyll were obtained during 10 min applying ultrasound-assisted extraction method. In contrast, the lowest yields of carotenoid and chlorophyll were undertaken by supercritical fluid extraction method.Conclusion: Ultrasound-assisted extraction method was demonstrated to be a promising tool to recover higher value-added compounds from D.salina. |
| first_indexed | 2024-12-23T19:40:52Z |
| format | Article |
| id | doaj.art-781bb217c1234790bd8e0e944fa0ce88 |
| institution | Directory Open Access Journal |
| issn | 2538-1873 2538-2829 |
| language | English |
| last_indexed | 2024-12-23T19:40:52Z |
| publishDate | 2021-12-01 |
| publisher | Shiraz University of Medical Sciences |
| record_format | Article |
| series | International Journal of Nutrition Sciences |
| spelling | doaj.art-781bb217c1234790bd8e0e944fa0ce882022-12-21T17:33:40ZengShiraz University of Medical SciencesInternational Journal of Nutrition Sciences2538-18732538-28292021-12-016420821510.30476/ijns.2021.93230.116248048Comparison of Different Methods for Carotenoid Extraction from Dunaliella SalinaMaryam Nejadmansouri0Mohammad-Taghi Golmakani1Mandana Famouri2Department of Food Science and Technology, School of Agriculture, Shiraz University, Shiraz, IranDepartment of Food Science and Technology, School of Agriculture, Shiraz University, Shiraz, IranDepartment of Food Science and Technology, School of Agriculture, Shiraz University, Shiraz, IranBackground: Dunaliella salina (D. salina) is a unicellular marine alga known for its carotenoid ingredient. Carotenoid has been used in food industry as a coloring additive and as an antioxidant. In addition, it has anticancer, antiaging, and immune-modulatory properties. Different methods have been used for extraction of carotenoids from algae. This study evaluated different extraction methods for carotenoid and comparedthe carotenoid yield and extraction time for procedures.Methods: In an experimental study, D. salina was isolated from Maharloo Lake in Shiraz, Iran. D. salina was centrifuged, dried, and suspended in methanol for further carotenoid extraction using different methods, namely ultrasound-assisted, supercritical fluid, microwave-assisted, electromantle, magnetic stirring, and maceration extraction methods.Results: Simultaneously, ultrasound-assisted, microwave-assisted, and electromantle extraction methods showed higher carotenoid yield and during a shorter time when compared with supercritical fluid, magnetic stirring, and maceration extraction methods. Since ultrasound-assisted extraction is a cold extraction method and ultrasound waves could release carotenoid and chlorophyll from broken cells to solvent quickly, the highest yields of carotenoid and chlorophyll were obtained during 10 min applying ultrasound-assisted extraction method. In contrast, the lowest yields of carotenoid and chlorophyll were undertaken by supercritical fluid extraction method.Conclusion: Ultrasound-assisted extraction method was demonstrated to be a promising tool to recover higher value-added compounds from D.salina.https://ijns.sums.ac.ir/article_48048_8fe8c0e7baf3a1026ca814b3a9ffe4e4.pdfcarotenoiddunaliella salinaextractionmicrowaveultrasound |
| spellingShingle | Maryam Nejadmansouri Mohammad-Taghi Golmakani Mandana Famouri Comparison of Different Methods for Carotenoid Extraction from Dunaliella Salina International Journal of Nutrition Sciences carotenoid dunaliella salina extraction microwave ultrasound |
| title | Comparison of Different Methods for Carotenoid Extraction from Dunaliella Salina |
| title_full | Comparison of Different Methods for Carotenoid Extraction from Dunaliella Salina |
| title_fullStr | Comparison of Different Methods for Carotenoid Extraction from Dunaliella Salina |
| title_full_unstemmed | Comparison of Different Methods for Carotenoid Extraction from Dunaliella Salina |
| title_short | Comparison of Different Methods for Carotenoid Extraction from Dunaliella Salina |
| title_sort | comparison of different methods for carotenoid extraction from dunaliella salina |
| topic | carotenoid dunaliella salina extraction microwave ultrasound |
| url | https://ijns.sums.ac.ir/article_48048_8fe8c0e7baf3a1026ca814b3a9ffe4e4.pdf |
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