Transcriptome Analysis of Jojoba (<i>Simmondsia chinensis</i>) during Seed Development and Liquid Wax Ester Biosynthesis
Jojoba is one of the main two known plant source of natural liquid wax ester for use in various applications, including cosmetics, pharmaceuticals, and biofuel. Due to the lack of transcriptomic and genomic data on lipid biosynthesis and accumulation, molecular marker breeding has been used to impro...
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MDPI AG
2020-05-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/9/5/588 |
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| author | Saqer S. Alotaibi Mona M. Elseehy Bandar S. Aljuaid Ahmed M. El-Shehawi |
| author_facet | Saqer S. Alotaibi Mona M. Elseehy Bandar S. Aljuaid Ahmed M. El-Shehawi |
| author_sort | Saqer S. Alotaibi |
| collection | DOAJ |
| description | Jojoba is one of the main two known plant source of natural liquid wax ester for use in various applications, including cosmetics, pharmaceuticals, and biofuel. Due to the lack of transcriptomic and genomic data on lipid biosynthesis and accumulation, molecular marker breeding has been used to improve jojoba oil production and quality. In the current study, the transcriptome of developing jojoba seeds was investigated using the Illunina NovaSeq 6000 system, 100 × 10<sup>6</sup> paired end reads, an average length of 100 bp, and a sequence depth of 12 Gb per sample. A total of 176,106 unigenes were detected with an average contig length of 201 bp. Gene Ontology (GO) showed that the detected unigenes were distributed in the three GO groups biological processes (BP, 5.53%), cellular component (CC, 6.06%), and molecular functions (MF, 5.88%) and distributed in 67 functional groups. The lipid biosynthesis pathway was established based on the expression of lipid biosynthesis genes, fatty acid (FA) biosynthesis, FA desaturation, FA elongation, fatty alcohol biosynthesis, triacylglycerol (TAG) biosynthesis, phospholipid metabolism, wax ester biosynthesis, and lipid transfer and storage genes. The detection of these categories of genes confirms the presence of an efficient lipid biosynthesis and accumulation system in developing jojoba seeds. The results of this study will significantly enhance the current understanding of wax ester biology in jojoba seeds and open new routes for the improvement of jojoba oil production and quality through biotechnology applications. |
| first_indexed | 2024-03-10T20:02:25Z |
| format | Article |
| id | doaj.art-781c2e59d8d845bd815fe9d43460ff7d |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T20:02:25Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-781c2e59d8d845bd815fe9d43460ff7d2023-11-19T23:28:28ZengMDPI AGPlants2223-77472020-05-019558810.3390/plants9050588Transcriptome Analysis of Jojoba (<i>Simmondsia chinensis</i>) during Seed Development and Liquid Wax Ester BiosynthesisSaqer S. Alotaibi0Mona M. Elseehy1Bandar S. Aljuaid2Ahmed M. El-Shehawi3Department of Biotechnology, Faculty of Science, Taif University, Taif 21974, Saudi ArabiaDepartment of Genetics, Faculty of Agriculture, University of Alexandria, Alexandria 21545, EgyptDepartment of Biotechnology, Faculty of Science, Taif University, Taif 21974, Saudi ArabiaDepartment of Biotechnology, Faculty of Science, Taif University, Taif 21974, Saudi ArabiaJojoba is one of the main two known plant source of natural liquid wax ester for use in various applications, including cosmetics, pharmaceuticals, and biofuel. Due to the lack of transcriptomic and genomic data on lipid biosynthesis and accumulation, molecular marker breeding has been used to improve jojoba oil production and quality. In the current study, the transcriptome of developing jojoba seeds was investigated using the Illunina NovaSeq 6000 system, 100 × 10<sup>6</sup> paired end reads, an average length of 100 bp, and a sequence depth of 12 Gb per sample. A total of 176,106 unigenes were detected with an average contig length of 201 bp. Gene Ontology (GO) showed that the detected unigenes were distributed in the three GO groups biological processes (BP, 5.53%), cellular component (CC, 6.06%), and molecular functions (MF, 5.88%) and distributed in 67 functional groups. The lipid biosynthesis pathway was established based on the expression of lipid biosynthesis genes, fatty acid (FA) biosynthesis, FA desaturation, FA elongation, fatty alcohol biosynthesis, triacylglycerol (TAG) biosynthesis, phospholipid metabolism, wax ester biosynthesis, and lipid transfer and storage genes. The detection of these categories of genes confirms the presence of an efficient lipid biosynthesis and accumulation system in developing jojoba seeds. The results of this study will significantly enhance the current understanding of wax ester biology in jojoba seeds and open new routes for the improvement of jojoba oil production and quality through biotechnology applications.https://www.mdpi.com/2223-7747/9/5/588jojoba<i>Simmondsia chinensis</i>transcriptomewax esterlipid metabolismtriglycerides |
| spellingShingle | Saqer S. Alotaibi Mona M. Elseehy Bandar S. Aljuaid Ahmed M. El-Shehawi Transcriptome Analysis of Jojoba (<i>Simmondsia chinensis</i>) during Seed Development and Liquid Wax Ester Biosynthesis Plants jojoba <i>Simmondsia chinensis</i> transcriptome wax ester lipid metabolism triglycerides |
| title | Transcriptome Analysis of Jojoba (<i>Simmondsia chinensis</i>) during Seed Development and Liquid Wax Ester Biosynthesis |
| title_full | Transcriptome Analysis of Jojoba (<i>Simmondsia chinensis</i>) during Seed Development and Liquid Wax Ester Biosynthesis |
| title_fullStr | Transcriptome Analysis of Jojoba (<i>Simmondsia chinensis</i>) during Seed Development and Liquid Wax Ester Biosynthesis |
| title_full_unstemmed | Transcriptome Analysis of Jojoba (<i>Simmondsia chinensis</i>) during Seed Development and Liquid Wax Ester Biosynthesis |
| title_short | Transcriptome Analysis of Jojoba (<i>Simmondsia chinensis</i>) during Seed Development and Liquid Wax Ester Biosynthesis |
| title_sort | transcriptome analysis of jojoba i simmondsia chinensis i during seed development and liquid wax ester biosynthesis |
| topic | jojoba <i>Simmondsia chinensis</i> transcriptome wax ester lipid metabolism triglycerides |
| url | https://www.mdpi.com/2223-7747/9/5/588 |
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