The ZuoJinWan formula inhibits glycolysis of cisplatin resistant gastric cancer cells via p53 acetylation
Introduction: Gastric cancer is one of the common malignancies worldwide, and drug resistance is a major factor contributing to the difficulty of treatment in gastric cancer. Zuojinwan (ZJW) has been found to exhibit a certain inhibitory effect on tumor cells. However, the molecular mechanisms of ZJ...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-03-01
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| Series: | Pharmacological Research - Modern Chinese Medicine |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667142523001331 |
| _version_ | 1797253912796856320 |
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| author | Zan Li Qiuxue Wu Zhenzhen Wei Yuli Zhang Bin Han Sailei Jia Hua Sui Qingfeng Tang |
| author_facet | Zan Li Qiuxue Wu Zhenzhen Wei Yuli Zhang Bin Han Sailei Jia Hua Sui Qingfeng Tang |
| author_sort | Zan Li |
| collection | DOAJ |
| description | Introduction: Gastric cancer is one of the common malignancies worldwide, and drug resistance is a major factor contributing to the difficulty of treatment in gastric cancer. Zuojinwan (ZJW) has been found to exhibit a certain inhibitory effect on tumor cells. However, the molecular mechanisms of ZJW reversing drug resistance in gastric cancer are still unclear. Method: Human gastric cancer cisplatin-resistant cells SGC-7901/DDP and BGC-823/DDP were divided into control groups, DDP groups (10 μg/mL), 2-DG (5 mM) groups, and ZJW (50 μg/mL) combined with DDP (10 μg/mL) groups. After 48 h of culture, cell proliferation inhibition rate, glucose uptake rate, ATP, and lactate production were detected. Following lentiviral transfection to overexpress GLUT1 and HDAC1, western blot analysis was employed to examine the expression of HDAC1, P53, Ace-p53, and glucose metabolism-related proteins such as GLUT1, LDHA, HK II in the cells. Results: The combination of ZJW and DDP significantly inhibits the proliferation and glycolysis of cisplatin resistant gastric cancer cells. Compared with the DDP group, the combination of ZJW and DDP exhibited a higher inhibition rate (P < 0.01), accompanied by a reduction in the expression of HDAC1 and P53 proteins. Conclusions: Zuojinwan can enhance the proliferation inhibition rate of SGC-7901/DDP and BGC-823/DDP cells, attenuate glycolysis, and reduce chemotherapy resistance. Its mechanism may be associated with the inhibition of HDAC1/P53 axis activity. |
| first_indexed | 2024-03-08T13:01:16Z |
| format | Article |
| id | doaj.art-781fe3a8c1a14f1f80b1808580c921a9 |
| institution | Directory Open Access Journal |
| issn | 2667-1425 |
| language | English |
| last_indexed | 2024-04-24T21:41:36Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Pharmacological Research - Modern Chinese Medicine |
| spelling | doaj.art-781fe3a8c1a14f1f80b1808580c921a92024-03-21T05:37:57ZengElsevierPharmacological Research - Modern Chinese Medicine2667-14252024-03-0110100347The ZuoJinWan formula inhibits glycolysis of cisplatin resistant gastric cancer cells via p53 acetylationZan Li0Qiuxue Wu1Zhenzhen Wei2Yuli Zhang3Bin Han4Sailei Jia5Hua Sui6Qingfeng Tang7Medical Experiment Center, Jiading Branch of Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201803, PR ChinaDepartment of Classic Traditional Chinese Medicine, Wuxi Affiliated Hospital of Nanjing University of Chinese Medicine, Wuxi 214000, PR ChinaMedical Experiment Center, Jiading Branch of Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201803, PR ChinaDepartment of Traditional Chinese Medicine, Jiading Branch of Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201803, PR ChinaThe Second Clinical Medical College of Henan University of Traditional Chinese Medicine, Zhengzhou 450000, PR ChinaThe Second Clinical Medical College of Henan University of Traditional Chinese Medicine, Zhengzhou 450000, PR ChinaMedical Experiment Center, Jiading Branch of Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201803, PR China; Corresponding author at: Medical Experiment Center, Jiading Branch of Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, 800 Huang jiahuayuan Road, Shanghai 201803, PR China.Nanxiang Branch of Ruijin Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai 201802, PR China; Corresponding author at: Nanxiang Branch of Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 495 Zhongren Road, Shanghai 201802, PR China.Introduction: Gastric cancer is one of the common malignancies worldwide, and drug resistance is a major factor contributing to the difficulty of treatment in gastric cancer. Zuojinwan (ZJW) has been found to exhibit a certain inhibitory effect on tumor cells. However, the molecular mechanisms of ZJW reversing drug resistance in gastric cancer are still unclear. Method: Human gastric cancer cisplatin-resistant cells SGC-7901/DDP and BGC-823/DDP were divided into control groups, DDP groups (10 μg/mL), 2-DG (5 mM) groups, and ZJW (50 μg/mL) combined with DDP (10 μg/mL) groups. After 48 h of culture, cell proliferation inhibition rate, glucose uptake rate, ATP, and lactate production were detected. Following lentiviral transfection to overexpress GLUT1 and HDAC1, western blot analysis was employed to examine the expression of HDAC1, P53, Ace-p53, and glucose metabolism-related proteins such as GLUT1, LDHA, HK II in the cells. Results: The combination of ZJW and DDP significantly inhibits the proliferation and glycolysis of cisplatin resistant gastric cancer cells. Compared with the DDP group, the combination of ZJW and DDP exhibited a higher inhibition rate (P < 0.01), accompanied by a reduction in the expression of HDAC1 and P53 proteins. Conclusions: Zuojinwan can enhance the proliferation inhibition rate of SGC-7901/DDP and BGC-823/DDP cells, attenuate glycolysis, and reduce chemotherapy resistance. Its mechanism may be associated with the inhibition of HDAC1/P53 axis activity.http://www.sciencedirect.com/science/article/pii/S2667142523001331ZuojinwanGlycolysisGastric cancerChemotherapy resistanceAce-p53 |
| spellingShingle | Zan Li Qiuxue Wu Zhenzhen Wei Yuli Zhang Bin Han Sailei Jia Hua Sui Qingfeng Tang The ZuoJinWan formula inhibits glycolysis of cisplatin resistant gastric cancer cells via p53 acetylation Pharmacological Research - Modern Chinese Medicine Zuojinwan Glycolysis Gastric cancer Chemotherapy resistance Ace-p53 |
| title | The ZuoJinWan formula inhibits glycolysis of cisplatin resistant gastric cancer cells via p53 acetylation |
| title_full | The ZuoJinWan formula inhibits glycolysis of cisplatin resistant gastric cancer cells via p53 acetylation |
| title_fullStr | The ZuoJinWan formula inhibits glycolysis of cisplatin resistant gastric cancer cells via p53 acetylation |
| title_full_unstemmed | The ZuoJinWan formula inhibits glycolysis of cisplatin resistant gastric cancer cells via p53 acetylation |
| title_short | The ZuoJinWan formula inhibits glycolysis of cisplatin resistant gastric cancer cells via p53 acetylation |
| title_sort | zuojinwan formula inhibits glycolysis of cisplatin resistant gastric cancer cells via p53 acetylation |
| topic | Zuojinwan Glycolysis Gastric cancer Chemotherapy resistance Ace-p53 |
| url | http://www.sciencedirect.com/science/article/pii/S2667142523001331 |
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