Isolation of Fucoxanthin from <i>Sargassum oligocystum</i> Montagne, 1845 Seaweed in Vietnam and Its Neuroprotective Activity
Fucoxanthin extracted and purified from Vietnamese <i>Sargassum oligocystum</i> Montagne, 1845 exhibits various biological activities. In this study, the ability of fucoxanthin to inhibit acetylcholinesterase (AChE), the antioxidant activities, and the expression of antioxidant enzymes w...
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MDPI AG
2023-08-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/11/8/2310 |
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| author | Dang Diem Hong Le Thi Thom Nguyen Cam Ha Ngo Thi Hoai Thu Hoang Thi Minh Hien Luu Thi Tam Nguyen Manh Dat Tran Mai Duc Nguyen Van Tru Nguyen Thi Minh Hang Ranga Rao Ambati |
| author_facet | Dang Diem Hong Le Thi Thom Nguyen Cam Ha Ngo Thi Hoai Thu Hoang Thi Minh Hien Luu Thi Tam Nguyen Manh Dat Tran Mai Duc Nguyen Van Tru Nguyen Thi Minh Hang Ranga Rao Ambati |
| author_sort | Dang Diem Hong |
| collection | DOAJ |
| description | Fucoxanthin extracted and purified from Vietnamese <i>Sargassum oligocystum</i> Montagne, 1845 exhibits various biological activities. In this study, the ability of fucoxanthin to inhibit acetylcholinesterase (AChE), the antioxidant activities, and the expression of antioxidant enzymes were investigated. Fucoxanthin isolated from Vietnamese <i>S. oligocystum</i> showed no cytotoxic effects; moreover, it exhibited AChE inhibitory activity (with an IC<sub>50</sub> value of 130.12 ± 6.65 μg mL<sup>−1</sup>) and antioxidant activity (with an IC<sub>50</sub> value of 3.42 ± 0.15 mg mL<sup>−1</sup>). At concentrations of 50 and 100 µg mL<sup>−1</sup>, fucoxanthin provided protection against amyloid β-protein fragment 25–35-induced neurotoxicity in a C6 neuronal cell line, and the survival of C6 cells was higher than 81.01% and 80.98%, respectively, compared to the control group (59%). Moreover, antioxidant enzyme activity and quantitative PCR analysis suggested that the neuroprotective effect of fucoxanthin resulted from regulation of the gene expression of antioxidant enzymes (CAT and GPx) and ER pathways (caspase-3 and Bax), as well as the promotion of expression of genes involved in PI3K/Akt signaling (GSK-3β), autophagy (p62 and ATG5), and the biosynthesis of ACh (VAChT and ChAT). Therefore, fucoxanthin extracted from the seaweed <i>S. oligocystum</i> in Vietnam is a potential feedstock source for the production of health foods that exert neuroprotective effects. |
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| language | English |
| last_indexed | 2024-03-09T03:22:31Z |
| publishDate | 2023-08-01 |
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| series | Biomedicines |
| spelling | doaj.art-7820f443c88e41dd86ffaaab1e0638272023-12-03T15:06:37ZengMDPI AGBiomedicines2227-90592023-08-01118231010.3390/biomedicines11082310Isolation of Fucoxanthin from <i>Sargassum oligocystum</i> Montagne, 1845 Seaweed in Vietnam and Its Neuroprotective ActivityDang Diem Hong0Le Thi Thom1Nguyen Cam Ha2Ngo Thi Hoai Thu3Hoang Thi Minh Hien4Luu Thi Tam5Nguyen Manh Dat6Tran Mai Duc7Nguyen Van Tru8Nguyen Thi Minh Hang9Ranga Rao Ambati10Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Str., Cau Giay, Hanoi 100000, VietnamInstitute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Str., Cau Giay, Hanoi 100000, VietnamInstitute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Str., Cau Giay, Hanoi 100000, VietnamInstitute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Str., Cau Giay, Hanoi 100000, VietnamInstitute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Str., Cau Giay, Hanoi 100000, VietnamInstitute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Str., Cau Giay, Hanoi 100000, VietnamInstitute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Str., Cau Giay, Hanoi 100000, VietnamNha Trang Institute of Technology Research and Application, Vietnam Academy of Science and Technology, Nha Trang 57100, VietnamInstitute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Str., Cau Giay, Hanoi 100000, VietnamInstitute of Marine Biochemistry, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Str., Cau Giay, Hanoi 100000, VietnamDepartment of Biotechnology, School of Biotechnology and Pharmaceutical Sciences, Vignan’s Foundation for Science, Technology and Research (Deemed to Be University), Vadlamudi, Guntur 522213, IndiaFucoxanthin extracted and purified from Vietnamese <i>Sargassum oligocystum</i> Montagne, 1845 exhibits various biological activities. In this study, the ability of fucoxanthin to inhibit acetylcholinesterase (AChE), the antioxidant activities, and the expression of antioxidant enzymes were investigated. Fucoxanthin isolated from Vietnamese <i>S. oligocystum</i> showed no cytotoxic effects; moreover, it exhibited AChE inhibitory activity (with an IC<sub>50</sub> value of 130.12 ± 6.65 μg mL<sup>−1</sup>) and antioxidant activity (with an IC<sub>50</sub> value of 3.42 ± 0.15 mg mL<sup>−1</sup>). At concentrations of 50 and 100 µg mL<sup>−1</sup>, fucoxanthin provided protection against amyloid β-protein fragment 25–35-induced neurotoxicity in a C6 neuronal cell line, and the survival of C6 cells was higher than 81.01% and 80.98%, respectively, compared to the control group (59%). Moreover, antioxidant enzyme activity and quantitative PCR analysis suggested that the neuroprotective effect of fucoxanthin resulted from regulation of the gene expression of antioxidant enzymes (CAT and GPx) and ER pathways (caspase-3 and Bax), as well as the promotion of expression of genes involved in PI3K/Akt signaling (GSK-3β), autophagy (p62 and ATG5), and the biosynthesis of ACh (VAChT and ChAT). Therefore, fucoxanthin extracted from the seaweed <i>S. oligocystum</i> in Vietnam is a potential feedstock source for the production of health foods that exert neuroprotective effects.https://www.mdpi.com/2227-9059/11/8/2310Alzheimer’sacetylcholinesterase inhibitoryβ-amyloid protein fragmentfucoxanthinhealth food for humans<i>Sargassum oligocystum</i> |
| spellingShingle | Dang Diem Hong Le Thi Thom Nguyen Cam Ha Ngo Thi Hoai Thu Hoang Thi Minh Hien Luu Thi Tam Nguyen Manh Dat Tran Mai Duc Nguyen Van Tru Nguyen Thi Minh Hang Ranga Rao Ambati Isolation of Fucoxanthin from <i>Sargassum oligocystum</i> Montagne, 1845 Seaweed in Vietnam and Its Neuroprotective Activity Biomedicines Alzheimer’s acetylcholinesterase inhibitory β-amyloid protein fragment fucoxanthin health food for humans <i>Sargassum oligocystum</i> |
| title | Isolation of Fucoxanthin from <i>Sargassum oligocystum</i> Montagne, 1845 Seaweed in Vietnam and Its Neuroprotective Activity |
| title_full | Isolation of Fucoxanthin from <i>Sargassum oligocystum</i> Montagne, 1845 Seaweed in Vietnam and Its Neuroprotective Activity |
| title_fullStr | Isolation of Fucoxanthin from <i>Sargassum oligocystum</i> Montagne, 1845 Seaweed in Vietnam and Its Neuroprotective Activity |
| title_full_unstemmed | Isolation of Fucoxanthin from <i>Sargassum oligocystum</i> Montagne, 1845 Seaweed in Vietnam and Its Neuroprotective Activity |
| title_short | Isolation of Fucoxanthin from <i>Sargassum oligocystum</i> Montagne, 1845 Seaweed in Vietnam and Its Neuroprotective Activity |
| title_sort | isolation of fucoxanthin from i sargassum oligocystum i montagne 1845 seaweed in vietnam and its neuroprotective activity |
| topic | Alzheimer’s acetylcholinesterase inhibitory β-amyloid protein fragment fucoxanthin health food for humans <i>Sargassum oligocystum</i> |
| url | https://www.mdpi.com/2227-9059/11/8/2310 |
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