Development and Application of Low-Cost and Eco-Sustainable Bio-Stimulant Containing a New Plant Growth-Promoting Strain Kosakonia pseudosacchari TL13
The use of beneficial microbes as inoculants able to improve fitness, growth and health of plants also in stress conditions is an attractive low-cost and eco-friendly alternative strategy to harmful chemical inputs. Thirteen potential plant growth-promoting bacteria were isolated from the rhizospher...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-08-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2020.02044/full |
| _version_ | 1818317293756612608 |
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| author | Ida Romano Valeria Ventorino Patrizia Ambrosino Antonino Testa Fatima Ezzahra Chouyia Fatima Ezzahra Chouyia Olimpia Pepe |
| author_facet | Ida Romano Valeria Ventorino Patrizia Ambrosino Antonino Testa Fatima Ezzahra Chouyia Fatima Ezzahra Chouyia Olimpia Pepe |
| author_sort | Ida Romano |
| collection | DOAJ |
| description | The use of beneficial microbes as inoculants able to improve fitness, growth and health of plants also in stress conditions is an attractive low-cost and eco-friendly alternative strategy to harmful chemical inputs. Thirteen potential plant growth-promoting bacteria were isolated from the rhizosphere of wheat plants cultivated under drought stress and nitrogen deficiency. Among these, the two isolates TL8 and TL13 showed multiple plant growth promotion activities as production of indole-3-acetic acid (IAA), siderophores, ammonia, and 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase production, the ability to solubilize phosphate as well as exerted antimicrobial activity against plant pathogens as Botrytis spp. and Phytophthora spp. The two selected strains were identified as Kosakonia pseudosacchari by sequencing of 16S rRNA gene. They resulted also tolerant to abiotic stress and were able to efficiently colonize plant roots as observed in vitro assay under fluorescence microscope. Based on the best PGP properties, the strain K. pseudosacchari TL13 was selected to develop a new microbial based formulate. A sustainable and environmentally friendly process for inoculant production was developed using agro-industrial by-products for microbial growth. Moreover, the application of K. pseudosacchari TL13- based formulates in pot experiment improved growth performance of maize plants. |
| first_indexed | 2024-12-13T09:35:02Z |
| format | Article |
| id | doaj.art-782aee03a9584f98b49468525bfcc48b |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-13T09:35:02Z |
| publishDate | 2020-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-782aee03a9584f98b49468525bfcc48b2022-12-21T23:52:24ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-08-011110.3389/fmicb.2020.02044557001Development and Application of Low-Cost and Eco-Sustainable Bio-Stimulant Containing a New Plant Growth-Promoting Strain Kosakonia pseudosacchari TL13Ida Romano0Valeria Ventorino1Patrizia Ambrosino2Antonino Testa3Fatima Ezzahra Chouyia4Fatima Ezzahra Chouyia5Olimpia Pepe6Department of Agricultural Sciences, University of Naples Federico II, Naples, ItalyDepartment of Agricultural Sciences, University of Naples Federico II, Naples, ItalyAgriges S.r.l. – Nutrizione Speciale per L’Agricoltura Biologica e Integrata, San Salvatore Telesino, ItalyDepartment of Agricultural Sciences, University of Naples Federico II, Naples, ItalyDepartment of Agricultural Sciences, University of Naples Federico II, Naples, ItalyDepartment of Biology, Faculty of Sciences and Techniques, Hassan II University of Casablanca, Casablanca, MoroccoDepartment of Agricultural Sciences, University of Naples Federico II, Naples, ItalyThe use of beneficial microbes as inoculants able to improve fitness, growth and health of plants also in stress conditions is an attractive low-cost and eco-friendly alternative strategy to harmful chemical inputs. Thirteen potential plant growth-promoting bacteria were isolated from the rhizosphere of wheat plants cultivated under drought stress and nitrogen deficiency. Among these, the two isolates TL8 and TL13 showed multiple plant growth promotion activities as production of indole-3-acetic acid (IAA), siderophores, ammonia, and 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase production, the ability to solubilize phosphate as well as exerted antimicrobial activity against plant pathogens as Botrytis spp. and Phytophthora spp. The two selected strains were identified as Kosakonia pseudosacchari by sequencing of 16S rRNA gene. They resulted also tolerant to abiotic stress and were able to efficiently colonize plant roots as observed in vitro assay under fluorescence microscope. Based on the best PGP properties, the strain K. pseudosacchari TL13 was selected to develop a new microbial based formulate. A sustainable and environmentally friendly process for inoculant production was developed using agro-industrial by-products for microbial growth. Moreover, the application of K. pseudosacchari TL13- based formulates in pot experiment improved growth performance of maize plants.https://www.frontiersin.org/article/10.3389/fmicb.2020.02044/fullinoculantPGPRrhizocompetencebio-formulateorganic by-products |
| spellingShingle | Ida Romano Valeria Ventorino Patrizia Ambrosino Antonino Testa Fatima Ezzahra Chouyia Fatima Ezzahra Chouyia Olimpia Pepe Development and Application of Low-Cost and Eco-Sustainable Bio-Stimulant Containing a New Plant Growth-Promoting Strain Kosakonia pseudosacchari TL13 Frontiers in Microbiology inoculant PGPR rhizocompetence bio-formulate organic by-products |
| title | Development and Application of Low-Cost and Eco-Sustainable Bio-Stimulant Containing a New Plant Growth-Promoting Strain Kosakonia pseudosacchari TL13 |
| title_full | Development and Application of Low-Cost and Eco-Sustainable Bio-Stimulant Containing a New Plant Growth-Promoting Strain Kosakonia pseudosacchari TL13 |
| title_fullStr | Development and Application of Low-Cost and Eco-Sustainable Bio-Stimulant Containing a New Plant Growth-Promoting Strain Kosakonia pseudosacchari TL13 |
| title_full_unstemmed | Development and Application of Low-Cost and Eco-Sustainable Bio-Stimulant Containing a New Plant Growth-Promoting Strain Kosakonia pseudosacchari TL13 |
| title_short | Development and Application of Low-Cost and Eco-Sustainable Bio-Stimulant Containing a New Plant Growth-Promoting Strain Kosakonia pseudosacchari TL13 |
| title_sort | development and application of low cost and eco sustainable bio stimulant containing a new plant growth promoting strain kosakonia pseudosacchari tl13 |
| topic | inoculant PGPR rhizocompetence bio-formulate organic by-products |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2020.02044/full |
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