Evaluation of chitinolytic enzymes activities during microbial biotransformation of Archachatina marginata exoskeleton to chitooligosaccharide
Natural macromolecules such as polysaccharides are bioactive substances obtained from the agricultural feed stocks and\ or crustacean shell wastes, thus chitin biomass as a rich renewable resource; is an additional abundant natural polysaccharide in the environment. Despite the recent interests in...
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The National Information and Documentation Centre (NIDOC) , Egypt
2022-04-01
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| Series: | Novel Research in Microbiology Journal |
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| Online Access: | https://nrmj.journals.ekb.eg/article_231601_c121754bb9f0a7dd78e822693d6676d9.pdf |
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| author | Simiat O. Jimoh Khadijat O. Adefaye Lateefah A. Arowolo Ramot B. Badmos-Oladapo |
| author_facet | Simiat O. Jimoh Khadijat O. Adefaye Lateefah A. Arowolo Ramot B. Badmos-Oladapo |
| author_sort | Simiat O. Jimoh |
| collection | DOAJ |
| description | Natural macromolecules such as polysaccharides are bioactive substances obtained from the agricultural feed stocks and\ or crustacean shell wastes, thus chitin biomass as a rich renewable resource; is an additional abundant natural polysaccharide in the environment. Despite the recent interests in the biomedical applications of chitin, complexity of chitosan and their derivatives in terms of controlling their chemical synthesis due to the formation of secondary compounds is a major problem, thus this research aimed to focus on the microbial biosynthesis of chitinous products and their derivatives from the seafood wastes. In the current research work; chitin, chitosan and chitooligosaccharides (COS) were biosynthesized from Archachatina marginata exoskeletons; through chemical deacetylation and microbial transformation techniques. During this study, Archachatina marginata exoskeleton was analyzed according to the method adopted by the Association of Official Analytical Chemists. In addition, a synthetic route for chitooligosaccharides production was investigated using a previously characterized Saccharomyces cerevisiae KR13 strain. The crude extract obtained after the biotransformation period was subjected to derivatization, and then the chitooligosaccharides yield, enzymes activities and their molecular weights were determined using Gas chromatography (GC). Moreover, the size exclusion chromatography revealed the presence of several enzymes, including chitin deacetylase; chitooligosaccharide deacetylase, chitinase and chitosanase; in addition to chitooligosaccharides derivatives such as; chitohexose, chitopentose, chitotetrose, chitotriose, chitobiose and chitosan with varying concentrations. Based on the results obtained in this study, manipulation of S. cerevisiae KR13 strain clarified the poor solubility of chitin and chitosan, which makes a challenge in using them in the biomedical applications, despite their important functional activities. |
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| issn | 2537-0286 2537-0294 |
| language | English |
| last_indexed | 2024-04-24T08:32:24Z |
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| publisher | The National Information and Documentation Centre (NIDOC) , Egypt |
| record_format | Article |
| series | Novel Research in Microbiology Journal |
| spelling | doaj.art-782e1a823a5a43eb807ebdbd8a3cec3d2024-04-16T19:24:58ZengThe National Information and Documentation Centre (NIDOC) , EgyptNovel Research in Microbiology Journal2537-02862537-02942022-04-01621530154210.21608/NRMJ.2022.231601Evaluation of chitinolytic enzymes activities during microbial biotransformation of Archachatina marginata exoskeleton to chitooligosaccharide Simiat O. Jimoh0Khadijat O. Adefaye1Lateefah A. Arowolo2Ramot B. Badmos-Oladapo3Department of Biological Sciences (Microbiology Programme), Fountain University, Osogbo, NigeriaDepartment of Biological Sciences (Microbiology Programme), Fountain University, Osogbo, NigeriaDepartment of Medicine and Surgery, Faculty of Clinical Sciences, Lagos State University, Nigeria Department of Biological Sciences (Microbiology Programme), Fountain University, Osogbo, NigeriaNatural macromolecules such as polysaccharides are bioactive substances obtained from the agricultural feed stocks and\ or crustacean shell wastes, thus chitin biomass as a rich renewable resource; is an additional abundant natural polysaccharide in the environment. Despite the recent interests in the biomedical applications of chitin, complexity of chitosan and their derivatives in terms of controlling their chemical synthesis due to the formation of secondary compounds is a major problem, thus this research aimed to focus on the microbial biosynthesis of chitinous products and their derivatives from the seafood wastes. In the current research work; chitin, chitosan and chitooligosaccharides (COS) were biosynthesized from Archachatina marginata exoskeletons; through chemical deacetylation and microbial transformation techniques. During this study, Archachatina marginata exoskeleton was analyzed according to the method adopted by the Association of Official Analytical Chemists. In addition, a synthetic route for chitooligosaccharides production was investigated using a previously characterized Saccharomyces cerevisiae KR13 strain. The crude extract obtained after the biotransformation period was subjected to derivatization, and then the chitooligosaccharides yield, enzymes activities and their molecular weights were determined using Gas chromatography (GC). Moreover, the size exclusion chromatography revealed the presence of several enzymes, including chitin deacetylase; chitooligosaccharide deacetylase, chitinase and chitosanase; in addition to chitooligosaccharides derivatives such as; chitohexose, chitopentose, chitotetrose, chitotriose, chitobiose and chitosan with varying concentrations. Based on the results obtained in this study, manipulation of S. cerevisiae KR13 strain clarified the poor solubility of chitin and chitosan, which makes a challenge in using them in the biomedical applications, despite their important functional activities.https://nrmj.journals.ekb.eg/article_231601_c121754bb9f0a7dd78e822693d6676d9.pdfchitooligosaccharidechitin deacetylasechitosanasesaccharomyces cerevisiae |
| spellingShingle | Simiat O. Jimoh Khadijat O. Adefaye Lateefah A. Arowolo Ramot B. Badmos-Oladapo Evaluation of chitinolytic enzymes activities during microbial biotransformation of Archachatina marginata exoskeleton to chitooligosaccharide Novel Research in Microbiology Journal chitooligosaccharide chitin deacetylase chitosanase saccharomyces cerevisiae |
| title | Evaluation of chitinolytic enzymes activities during microbial biotransformation of Archachatina marginata exoskeleton to chitooligosaccharide |
| title_full | Evaluation of chitinolytic enzymes activities during microbial biotransformation of Archachatina marginata exoskeleton to chitooligosaccharide |
| title_fullStr | Evaluation of chitinolytic enzymes activities during microbial biotransformation of Archachatina marginata exoskeleton to chitooligosaccharide |
| title_full_unstemmed | Evaluation of chitinolytic enzymes activities during microbial biotransformation of Archachatina marginata exoskeleton to chitooligosaccharide |
| title_short | Evaluation of chitinolytic enzymes activities during microbial biotransformation of Archachatina marginata exoskeleton to chitooligosaccharide |
| title_sort | evaluation of chitinolytic enzymes activities during microbial biotransformation of archachatina marginata exoskeleton to chitooligosaccharide |
| topic | chitooligosaccharide chitin deacetylase chitosanase saccharomyces cerevisiae |
| url | https://nrmj.journals.ekb.eg/article_231601_c121754bb9f0a7dd78e822693d6676d9.pdf |
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