Paradigm of coinfection with viral and bacterial agents causing bovine respiratory disease complex (BRDC) in Central Serbia
Viral and bacterial agents causing bovine respiratory disease complex (BRDC) were examined over a five-year period (2015-2019). Thirty-eight farms of beef and dairy cattle from Central Serbia which showed symptoms of BRDC were observed. A total of 118 nasal swab specimens collected from diseased bul...
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| Format: | Article |
| Language: | English |
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University of Kragujevac, Faculty of Agronomy, Cacak
2020-01-01
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| Series: | Acta Agriculturae Serbica |
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| Online Access: | https://scindeks-clanci.ceon.rs/data/pdf/0354-9542/2020/0354-95422049083M.pdf |
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| author | Matović Kazimir Kurćubić Vladimir S. Đoković Radojica D. Petrović Miloš Ž. Šekler Milanko Tešović Bojana |
| author_facet | Matović Kazimir Kurćubić Vladimir S. Đoković Radojica D. Petrović Miloš Ž. Šekler Milanko Tešović Bojana |
| author_sort | Matović Kazimir |
| collection | DOAJ |
| description | Viral and bacterial agents causing bovine respiratory disease complex (BRDC) were examined over a five-year period (2015-2019). Thirty-eight farms of beef and dairy cattle from Central Serbia which showed symptoms of BRDC were observed. A total of 118 nasal swab specimens collected from diseased bullocks (n=55), cows (n=18) and calves (n=45) were chosen for the isolation of bacteria and identification by aerobic cultivation. The most commonly isolated bacterial pathogen was Pasteurella multocida (72.88% positive samples). Disk-diffusion method (antibiogram) showed that Amoxiclav and Enrofloxacin were the most efficient antibiotics against Pasteurella multocida isolates (66.67% and 54.17%, respectively). From all examined samples, using the Real Time RT-PCR and PCR methods, we determined the genome sequences of bovine respiratory syncytial virus (BRSV-34.78% of tested samples (8/23), bovine viral diarrhea virus (BVDV, in 34.37% (11/32) and Mycoplasma spp. in 33.33% (8/24). The genome of BoHV-1 virus was not detected in any of the 19 samples tested by Real Time PCR method. |
| first_indexed | 2024-12-11T18:48:42Z |
| format | Article |
| id | doaj.art-782ff7e01d3b41d281585e51fb7dddbf |
| institution | Directory Open Access Journal |
| issn | 0354-9542 2560-3140 |
| language | English |
| last_indexed | 2024-12-11T18:48:42Z |
| publishDate | 2020-01-01 |
| publisher | University of Kragujevac, Faculty of Agronomy, Cacak |
| record_format | Article |
| series | Acta Agriculturae Serbica |
| spelling | doaj.art-782ff7e01d3b41d281585e51fb7dddbf2022-12-22T00:54:22ZengUniversity of Kragujevac, Faculty of Agronomy, CacakActa Agriculturae Serbica0354-95422560-31402020-01-01254983870354-95422049083MParadigm of coinfection with viral and bacterial agents causing bovine respiratory disease complex (BRDC) in Central SerbiaMatović Kazimir0Kurćubić Vladimir S.1https://orcid.org/0000-0003-2016-7345Đoković Radojica D.2Petrović Miloš Ž.3Šekler Milanko4Tešović Bojana5Veterinary Specialized Institute "Kraljevo", Kraljevo, SerbiaUniversity of Kragujevac, Faculty of Agronomy in Čačak, Čačak, SerbiaUniversity of Kragujevac, Faculty of Agronomy in Čačak, Čačak, SerbiaUniversity of Kragujevac, Faculty of Agronomy in Čačak, Čačak, SerbiaVeterinary Specialized Institute "Kraljevo", Kraljevo, SerbiaVeterinary Specialized Institute "Kraljevo", Kraljevo, SerbiaViral and bacterial agents causing bovine respiratory disease complex (BRDC) were examined over a five-year period (2015-2019). Thirty-eight farms of beef and dairy cattle from Central Serbia which showed symptoms of BRDC were observed. A total of 118 nasal swab specimens collected from diseased bullocks (n=55), cows (n=18) and calves (n=45) were chosen for the isolation of bacteria and identification by aerobic cultivation. The most commonly isolated bacterial pathogen was Pasteurella multocida (72.88% positive samples). Disk-diffusion method (antibiogram) showed that Amoxiclav and Enrofloxacin were the most efficient antibiotics against Pasteurella multocida isolates (66.67% and 54.17%, respectively). From all examined samples, using the Real Time RT-PCR and PCR methods, we determined the genome sequences of bovine respiratory syncytial virus (BRSV-34.78% of tested samples (8/23), bovine viral diarrhea virus (BVDV, in 34.37% (11/32) and Mycoplasma spp. in 33.33% (8/24). The genome of BoHV-1 virus was not detected in any of the 19 samples tested by Real Time PCR method.https://scindeks-clanci.ceon.rs/data/pdf/0354-9542/2020/0354-95422049083M.pdfbovine respiratory disease complex (brdc)virusesbacteriamycoplasma spp.molecular methodsisolationresistance |
| spellingShingle | Matović Kazimir Kurćubić Vladimir S. Đoković Radojica D. Petrović Miloš Ž. Šekler Milanko Tešović Bojana Paradigm of coinfection with viral and bacterial agents causing bovine respiratory disease complex (BRDC) in Central Serbia Acta Agriculturae Serbica bovine respiratory disease complex (brdc) viruses bacteria mycoplasma spp. molecular methods isolation resistance |
| title | Paradigm of coinfection with viral and bacterial agents causing bovine respiratory disease complex (BRDC) in Central Serbia |
| title_full | Paradigm of coinfection with viral and bacterial agents causing bovine respiratory disease complex (BRDC) in Central Serbia |
| title_fullStr | Paradigm of coinfection with viral and bacterial agents causing bovine respiratory disease complex (BRDC) in Central Serbia |
| title_full_unstemmed | Paradigm of coinfection with viral and bacterial agents causing bovine respiratory disease complex (BRDC) in Central Serbia |
| title_short | Paradigm of coinfection with viral and bacterial agents causing bovine respiratory disease complex (BRDC) in Central Serbia |
| title_sort | paradigm of coinfection with viral and bacterial agents causing bovine respiratory disease complex brdc in central serbia |
| topic | bovine respiratory disease complex (brdc) viruses bacteria mycoplasma spp. molecular methods isolation resistance |
| url | https://scindeks-clanci.ceon.rs/data/pdf/0354-9542/2020/0354-95422049083M.pdf |
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