Quantitative Nanomechanical Analysis of Small Extracellular Vesicles for Tumor Malignancy Indication
Abstract The nanomechanical properties of tumor‐derived small extracellular vesicles (sEVs) are essential to cancer progression. Here, nanoindentation is utilized on atomic force microscopy (AFM) to quantitatively investigate the nanomechanical properties of human breast cancer cell‐derived sEVs at...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2021-09-01
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| Series: | Advanced Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/advs.202100825 |
| _version_ | 1818738293957722112 |
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| author | Siyuan Ye Wenzhe Li Huayi Wang Ling Zhu Chen Wang Yanlian Yang |
| author_facet | Siyuan Ye Wenzhe Li Huayi Wang Ling Zhu Chen Wang Yanlian Yang |
| author_sort | Siyuan Ye |
| collection | DOAJ |
| description | Abstract The nanomechanical properties of tumor‐derived small extracellular vesicles (sEVs) are essential to cancer progression. Here, nanoindentation is utilized on atomic force microscopy (AFM) to quantitatively investigate the nanomechanical properties of human breast cancer cell‐derived sEVs at single vesicle level and explore their relationship with tumor malignancy and vesicle size. It is demonstrated that the stiffness of the sEVs results from the combined contribution of the bending modulus and osmotic pressure of the sEVs. The stiffness and osmotic pressure increase with increasing malignancy of the sEVs and decrease with increasing size of the sEVs. The bending modulus decreases with increasing malignancy of the sEVs and is lower in smaller sEVs. This study builds relationship between the nanomechanical signature of the sEV and tumor malignancy, adding information for better understanding cancer mechanobiology. |
| first_indexed | 2024-12-18T01:06:39Z |
| format | Article |
| id | doaj.art-7831d16433914fe88b7f6f576569421b |
| institution | Directory Open Access Journal |
| issn | 2198-3844 |
| language | English |
| last_indexed | 2024-12-18T01:06:39Z |
| publishDate | 2021-09-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj.art-7831d16433914fe88b7f6f576569421b2022-12-21T21:26:13ZengWileyAdvanced Science2198-38442021-09-01818n/an/a10.1002/advs.202100825Quantitative Nanomechanical Analysis of Small Extracellular Vesicles for Tumor Malignancy IndicationSiyuan Ye0Wenzhe Li1Huayi Wang2Ling Zhu3Chen Wang4Yanlian Yang5CAS Key Laboratory of Standardization and Measurement for Nanotechnology CAS Key Laboratory of Biological Effects of Nanomaterials and Nanosafety CAS Center for Excellence in Nanoscience National Center for Nanoscience and Technology Beijing 100190 P. R. ChinaCAS Key Laboratory of Standardization and Measurement for Nanotechnology CAS Key Laboratory of Biological Effects of Nanomaterials and Nanosafety CAS Center for Excellence in Nanoscience National Center for Nanoscience and Technology Beijing 100190 P. R. ChinaCAS Key Laboratory of Standardization and Measurement for Nanotechnology CAS Key Laboratory of Biological Effects of Nanomaterials and Nanosafety CAS Center for Excellence in Nanoscience National Center for Nanoscience and Technology Beijing 100190 P. R. ChinaCAS Key Laboratory of Standardization and Measurement for Nanotechnology CAS Key Laboratory of Biological Effects of Nanomaterials and Nanosafety CAS Center for Excellence in Nanoscience National Center for Nanoscience and Technology Beijing 100190 P. R. ChinaCAS Key Laboratory of Standardization and Measurement for Nanotechnology CAS Key Laboratory of Biological Effects of Nanomaterials and Nanosafety CAS Center for Excellence in Nanoscience National Center for Nanoscience and Technology Beijing 100190 P. R. ChinaCAS Key Laboratory of Standardization and Measurement for Nanotechnology CAS Key Laboratory of Biological Effects of Nanomaterials and Nanosafety CAS Center for Excellence in Nanoscience National Center for Nanoscience and Technology Beijing 100190 P. R. ChinaAbstract The nanomechanical properties of tumor‐derived small extracellular vesicles (sEVs) are essential to cancer progression. Here, nanoindentation is utilized on atomic force microscopy (AFM) to quantitatively investigate the nanomechanical properties of human breast cancer cell‐derived sEVs at single vesicle level and explore their relationship with tumor malignancy and vesicle size. It is demonstrated that the stiffness of the sEVs results from the combined contribution of the bending modulus and osmotic pressure of the sEVs. The stiffness and osmotic pressure increase with increasing malignancy of the sEVs and decrease with increasing size of the sEVs. The bending modulus decreases with increasing malignancy of the sEVs and is lower in smaller sEVs. This study builds relationship between the nanomechanical signature of the sEV and tumor malignancy, adding information for better understanding cancer mechanobiology.https://doi.org/10.1002/advs.202100825atomic force microscopycancer mechanobiologyextracellular vesiclesnanoindentationnanomechanical propertytumor |
| spellingShingle | Siyuan Ye Wenzhe Li Huayi Wang Ling Zhu Chen Wang Yanlian Yang Quantitative Nanomechanical Analysis of Small Extracellular Vesicles for Tumor Malignancy Indication Advanced Science atomic force microscopy cancer mechanobiology extracellular vesicles nanoindentation nanomechanical property tumor |
| title | Quantitative Nanomechanical Analysis of Small Extracellular Vesicles for Tumor Malignancy Indication |
| title_full | Quantitative Nanomechanical Analysis of Small Extracellular Vesicles for Tumor Malignancy Indication |
| title_fullStr | Quantitative Nanomechanical Analysis of Small Extracellular Vesicles for Tumor Malignancy Indication |
| title_full_unstemmed | Quantitative Nanomechanical Analysis of Small Extracellular Vesicles for Tumor Malignancy Indication |
| title_short | Quantitative Nanomechanical Analysis of Small Extracellular Vesicles for Tumor Malignancy Indication |
| title_sort | quantitative nanomechanical analysis of small extracellular vesicles for tumor malignancy indication |
| topic | atomic force microscopy cancer mechanobiology extracellular vesicles nanoindentation nanomechanical property tumor |
| url | https://doi.org/10.1002/advs.202100825 |
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