Integrative analysis of green ash phloem transcripts and proteins during an emerald ash borer infestation
Abstract Background Emerald ash borer (Agrilus planipennis; EAB) is an Asian insect species that has been invasive to North America for 20 years. During this time, the emerald ash borer has killed tens of millions of American ash (Fraxinus spp) trees. Understanding the inherent defenses of susceptib...
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| Format: | Article |
| Language: | English |
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BMC
2023-03-01
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| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12870-023-04108-y |
| _version_ | 1827984770863202304 |
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| author | Christine C. Chiu Gervais Pelletier Juliana Stival Sena Florence Roux-Dalvai Julien Prunier Arnaud Droit Armand Séguin |
| author_facet | Christine C. Chiu Gervais Pelletier Juliana Stival Sena Florence Roux-Dalvai Julien Prunier Arnaud Droit Armand Séguin |
| author_sort | Christine C. Chiu |
| collection | DOAJ |
| description | Abstract Background Emerald ash borer (Agrilus planipennis; EAB) is an Asian insect species that has been invasive to North America for 20 years. During this time, the emerald ash borer has killed tens of millions of American ash (Fraxinus spp) trees. Understanding the inherent defenses of susceptible American ash trees will provide information to breed new resistant varieties of ash trees. Results We have performed RNA-seq on naturally infested green ash (F. pennsylvanica) trees at low, medium and high levels of increasing EAB infestation and proteomics on low and high levels of EAB infestation. Most significant transcript changes we detected occurred between the comparison of medium and high levels of EAB infestation, indicating that the tree is not responding to EAB until it is highly infested. Our integrative analysis of the RNA-Seq and proteomics data identified 14 proteins and 4 transcripts that contribute most to the difference between highly infested and low infested trees. Conclusions The putative functions of these transcripts and proteins suggests roles of phenylpropanoid biosynthesis and oxidation, chitinase activity, pectinesterase activity, strigolactone signaling, and protein turnover. |
| first_indexed | 2024-04-09T23:04:10Z |
| format | Article |
| id | doaj.art-78338459be904c7abb2485c6ae13231f |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-04-09T23:04:10Z |
| publishDate | 2023-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-78338459be904c7abb2485c6ae13231f2023-03-22T10:49:29ZengBMCBMC Plant Biology1471-22292023-03-0123111310.1186/s12870-023-04108-yIntegrative analysis of green ash phloem transcripts and proteins during an emerald ash borer infestationChristine C. Chiu0Gervais Pelletier1Juliana Stival Sena2Florence Roux-Dalvai3Julien Prunier4Arnaud Droit5Armand Séguin6Laurentian Forestry Centre, Canadian Forest Service, Natural Resources CanadaLaurentian Forestry Centre, Canadian Forest Service, Natural Resources CanadaLaurentian Forestry Centre, Canadian Forest Service, Natural Resources CanadaCHU de Québec–Laval University Research Centre, Université LavalCHU de Québec–Laval University Research Centre, Université LavalCHU de Québec–Laval University Research Centre, Université LavalLaurentian Forestry Centre, Canadian Forest Service, Natural Resources CanadaAbstract Background Emerald ash borer (Agrilus planipennis; EAB) is an Asian insect species that has been invasive to North America for 20 years. During this time, the emerald ash borer has killed tens of millions of American ash (Fraxinus spp) trees. Understanding the inherent defenses of susceptible American ash trees will provide information to breed new resistant varieties of ash trees. Results We have performed RNA-seq on naturally infested green ash (F. pennsylvanica) trees at low, medium and high levels of increasing EAB infestation and proteomics on low and high levels of EAB infestation. Most significant transcript changes we detected occurred between the comparison of medium and high levels of EAB infestation, indicating that the tree is not responding to EAB until it is highly infested. Our integrative analysis of the RNA-Seq and proteomics data identified 14 proteins and 4 transcripts that contribute most to the difference between highly infested and low infested trees. Conclusions The putative functions of these transcripts and proteins suggests roles of phenylpropanoid biosynthesis and oxidation, chitinase activity, pectinesterase activity, strigolactone signaling, and protein turnover.https://doi.org/10.1186/s12870-023-04108-yEmerald ash borerFraxinusPlant defenseTranscriptomicsProteomicsPlant–insect interactions |
| spellingShingle | Christine C. Chiu Gervais Pelletier Juliana Stival Sena Florence Roux-Dalvai Julien Prunier Arnaud Droit Armand Séguin Integrative analysis of green ash phloem transcripts and proteins during an emerald ash borer infestation BMC Plant Biology Emerald ash borer Fraxinus Plant defense Transcriptomics Proteomics Plant–insect interactions |
| title | Integrative analysis of green ash phloem transcripts and proteins during an emerald ash borer infestation |
| title_full | Integrative analysis of green ash phloem transcripts and proteins during an emerald ash borer infestation |
| title_fullStr | Integrative analysis of green ash phloem transcripts and proteins during an emerald ash borer infestation |
| title_full_unstemmed | Integrative analysis of green ash phloem transcripts and proteins during an emerald ash borer infestation |
| title_short | Integrative analysis of green ash phloem transcripts and proteins during an emerald ash borer infestation |
| title_sort | integrative analysis of green ash phloem transcripts and proteins during an emerald ash borer infestation |
| topic | Emerald ash borer Fraxinus Plant defense Transcriptomics Proteomics Plant–insect interactions |
| url | https://doi.org/10.1186/s12870-023-04108-y |
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