Prevalence rate of Klebsiella pneumoniae in the intensive care unit: epidemiology and molecular characteristics
Klebsiella pneumoniae carbapenemase producing bacteria are defined as a group of Gramnegative bacilli that are highly resistant to drugs. The resistance of pathogens of the Enterobacteriaceae family to β-lactam antibiotics such as carbapenems is considered a major threat in the medical field. The ma...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
PAGEPress Publications
2018-06-01
|
| Series: | Journal of Biological Research |
| Subjects: | |
| Online Access: | https://www.pagepressjournals.org/index.php/jbr/article/view/7437 |
| _version_ | 1818966917167185920 |
|---|---|
| author | Soad A. Abdallah Sanaa S. Zaki Safa S. Hafez Elham E. Moustafa |
| author_facet | Soad A. Abdallah Sanaa S. Zaki Safa S. Hafez Elham E. Moustafa |
| author_sort | Soad A. Abdallah |
| collection | DOAJ |
| description | Klebsiella pneumoniae carbapenemase producing bacteria are defined as a group of Gramnegative bacilli that are highly resistant to drugs. The resistance of pathogens of the Enterobacteriaceae family to β-lactam antibiotics such as carbapenems is considered a major threat in the medical field. The main aim of the current study was to explore and confirm the occurrence of carbapenemase producing K. pneumoniae in the Intensive Care Unit (ICU) in different hospital environment sample sites of two Egyptian hospitals in Cairo. Isolates were collected and the screening criteria of carbapenemase producing bacteria was followed by the investigators in order to record the antimicrobial resistance patterns of all isolates in addition to the molecular identification using the 16S rDNA. This study determined the sites responsible for the spreading of carbapenem-resistant K. pneumoniae including suction tubes, ventilator tubes, instrument tables and beds. All K. pneumoniae isolates collected from the ICU of both hospitals were resistant to oxacillin, meropenem and ceftazidime. Moreover, 16S rDNA gene sequence was used to study bacterial phylogeny and taxonomy for all K. pneumoniae isolates; the accession number of all isolates is reported. We concluded that infection control department policies in each hospital should be reinforced to avoid the escalation of K. pneumoniae as nosocomial infections in hospitals. This study should be repeated in other hospitals (especially the public hospitals) to assess the level of the problem. |
| first_indexed | 2024-12-20T13:40:31Z |
| format | Article |
| id | doaj.art-78366a3fc2254b4ca3ab3fc48196ff05 |
| institution | Directory Open Access Journal |
| issn | 1826-8838 2284-0230 |
| language | English |
| last_indexed | 2024-12-20T13:40:31Z |
| publishDate | 2018-06-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | Journal of Biological Research |
| spelling | doaj.art-78366a3fc2254b4ca3ab3fc48196ff052022-12-21T19:38:50ZengPAGEPress PublicationsJournal of Biological Research1826-88382284-02302018-06-0191110.4081/jbr.2018.74375750Prevalence rate of Klebsiella pneumoniae in the intensive care unit: epidemiology and molecular characteristicsSoad A. Abdallah0Sanaa S. Zaki1Safa S. Hafez2Elham E. Moustafa3Department of Botany, Faculty of Women for Arts, Science and Education, Ain Shams University, CairoDepartment of Botany, Faculty of Women for Arts, Science and Education, Ain Shams University, CairoDepartment of Botany, Faculty of Women for Arts, Science and Education, Ain Shams University, CairoDepartment of Botany, Faculty of Women for Arts, Science and Education, Ain Shams University, CairoKlebsiella pneumoniae carbapenemase producing bacteria are defined as a group of Gramnegative bacilli that are highly resistant to drugs. The resistance of pathogens of the Enterobacteriaceae family to β-lactam antibiotics such as carbapenems is considered a major threat in the medical field. The main aim of the current study was to explore and confirm the occurrence of carbapenemase producing K. pneumoniae in the Intensive Care Unit (ICU) in different hospital environment sample sites of two Egyptian hospitals in Cairo. Isolates were collected and the screening criteria of carbapenemase producing bacteria was followed by the investigators in order to record the antimicrobial resistance patterns of all isolates in addition to the molecular identification using the 16S rDNA. This study determined the sites responsible for the spreading of carbapenem-resistant K. pneumoniae including suction tubes, ventilator tubes, instrument tables and beds. All K. pneumoniae isolates collected from the ICU of both hospitals were resistant to oxacillin, meropenem and ceftazidime. Moreover, 16S rDNA gene sequence was used to study bacterial phylogeny and taxonomy for all K. pneumoniae isolates; the accession number of all isolates is reported. We concluded that infection control department policies in each hospital should be reinforced to avoid the escalation of K. pneumoniae as nosocomial infections in hospitals. This study should be repeated in other hospitals (especially the public hospitals) to assess the level of the problem.https://www.pagepressjournals.org/index.php/jbr/article/view/7437Intensive care unit pathogensInanimate sourcesAntibiotic resistant K. pneumoniaeCarbapenemase. |
| spellingShingle | Soad A. Abdallah Sanaa S. Zaki Safa S. Hafez Elham E. Moustafa Prevalence rate of Klebsiella pneumoniae in the intensive care unit: epidemiology and molecular characteristics Journal of Biological Research Intensive care unit pathogens Inanimate sources Antibiotic resistant K. pneumoniae Carbapenemase. |
| title | Prevalence rate of Klebsiella pneumoniae in the intensive care unit: epidemiology and molecular characteristics |
| title_full | Prevalence rate of Klebsiella pneumoniae in the intensive care unit: epidemiology and molecular characteristics |
| title_fullStr | Prevalence rate of Klebsiella pneumoniae in the intensive care unit: epidemiology and molecular characteristics |
| title_full_unstemmed | Prevalence rate of Klebsiella pneumoniae in the intensive care unit: epidemiology and molecular characteristics |
| title_short | Prevalence rate of Klebsiella pneumoniae in the intensive care unit: epidemiology and molecular characteristics |
| title_sort | prevalence rate of klebsiella pneumoniae in the intensive care unit epidemiology and molecular characteristics |
| topic | Intensive care unit pathogens Inanimate sources Antibiotic resistant K. pneumoniae Carbapenemase. |
| url | https://www.pagepressjournals.org/index.php/jbr/article/view/7437 |
| work_keys_str_mv | AT soadaabdallah prevalencerateofklebsiellapneumoniaeintheintensivecareunitepidemiologyandmolecularcharacteristics AT sanaaszaki prevalencerateofklebsiellapneumoniaeintheintensivecareunitepidemiologyandmolecularcharacteristics AT safashafez prevalencerateofklebsiellapneumoniaeintheintensivecareunitepidemiologyandmolecularcharacteristics AT elhamemoustafa prevalencerateofklebsiellapneumoniaeintheintensivecareunitepidemiologyandmolecularcharacteristics |