Antimicrobial Susceptibility and AP-PCR Typing of Acine¬tobac-ter Spp. Strains
Background: Acinetobacter spp., as important opportunistic pathogens, have been found to be responsible for an increas­ing number of nosocomial infections. This study was undertaken to investigate the antimicrobial susceptibility and molecular typing of Iranian isolates of A. baumannii....
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2007-10-01
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| Series: | Iranian Journal of Public Health |
| Subjects: | |
| Online Access: | http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/4255.pdf&manuscript_id=4255 |
| _version_ | 1818740398194950144 |
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| author | R Ranjbar N Sadeghifard A Ahmadi M Izadi J Zaeimi- Yazdi A Ghasemi M Qurbanalizadgan A Rostamzad |
| author_facet | R Ranjbar N Sadeghifard A Ahmadi M Izadi J Zaeimi- Yazdi A Ghasemi M Qurbanalizadgan A Rostamzad |
| author_sort | R Ranjbar |
| collection | DOAJ |
| description | Background: Acinetobacter spp., as important opportunistic pathogens, have been found to be responsible for an increas­ing number of nosocomial infections. This study was undertaken to investigate the antimicrobial susceptibility and molecular typing of Iranian isolates of A. baumannii. Methods: The study was conducted over a period of 19 months in three hospitals in Tehran, Iran. Acinetobacter spp. were isolated from different clinical specimens using standard bacteriological methods. Antimicrobial susceptibility test was performed according to the standard CLSI guideline using 17 antibiotic disks. The AP-PCR fingerprinting was car­ried out using ARB11 primer. The PCR product was run and visualized in 2% agarose gels and stained with ethidium bro­mide. The AP-PCR profiles were grouped depending on the patterns of the amplified bands.Results: Sixty seven strains of Acinetobacter spp. (including 21 A. baumannii and 46 non- A. baumannii) were isolated. The sources of these isolates were blood, urine, wound, and respiratory tract.  A. baumannii isolates were further studied. Re­sults showed that all A. baumannii isolates were resistant to at least 11 antibiotics tested. AP-PCR analysis of A. bauman­nii strains resulted in 7 different patterns. The dominant AP-PCR pattern was E (57.1%). Conclusion: Acinetobacter spp. are still important nosocomial pathogens in the region studied and most of isolates were multi-drug resistant. Our results also indicate that the AP-PCR technique represents a rapid and simple means for typing of A. baumannii. |
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| format | Article |
| id | doaj.art-7836c3c6490e403f85da85312d8cb59e |
| institution | Directory Open Access Journal |
| issn | 2251-6085 |
| language | English |
| last_indexed | 2024-12-18T01:40:05Z |
| publishDate | 2007-10-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Public Health |
| spelling | doaj.art-7836c3c6490e403f85da85312d8cb59e2022-12-21T21:25:20ZengTehran University of Medical SciencesIranian Journal of Public Health2251-60852007-10-013645056Antimicrobial Susceptibility and AP-PCR Typing of Acine¬tobac-ter Spp. StrainsR RanjbarN SadeghifardA AhmadiM IzadiJ Zaeimi- YazdiA GhasemiM QurbanalizadganA RostamzadBackground: Acinetobacter spp., as important opportunistic pathogens, have been found to be responsible for an increas­ing number of nosocomial infections. This study was undertaken to investigate the antimicrobial susceptibility and molecular typing of Iranian isolates of A. baumannii. Methods: The study was conducted over a period of 19 months in three hospitals in Tehran, Iran. Acinetobacter spp. were isolated from different clinical specimens using standard bacteriological methods. Antimicrobial susceptibility test was performed according to the standard CLSI guideline using 17 antibiotic disks. The AP-PCR fingerprinting was car­ried out using ARB11 primer. The PCR product was run and visualized in 2% agarose gels and stained with ethidium bro­mide. The AP-PCR profiles were grouped depending on the patterns of the amplified bands.Results: Sixty seven strains of Acinetobacter spp. (including 21 A. baumannii and 46 non- A. baumannii) were isolated. The sources of these isolates were blood, urine, wound, and respiratory tract.  A. baumannii isolates were further studied. Re­sults showed that all A. baumannii isolates were resistant to at least 11 antibiotics tested. AP-PCR analysis of A. bauman­nii strains resulted in 7 different patterns. The dominant AP-PCR pattern was E (57.1%). Conclusion: Acinetobacter spp. are still important nosocomial pathogens in the region studied and most of isolates were multi-drug resistant. Our results also indicate that the AP-PCR technique represents a rapid and simple means for typing of A. baumannii.http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/4255.pdf&manuscript_id=4255Acinetobacter baumannii |
| spellingShingle | R Ranjbar N Sadeghifard A Ahmadi M Izadi J Zaeimi- Yazdi A Ghasemi M Qurbanalizadgan A Rostamzad Antimicrobial Susceptibility and AP-PCR Typing of Acine¬tobac-ter Spp. Strains Iranian Journal of Public Health Acinetobacter baumannii |
| title | Antimicrobial Susceptibility and AP-PCR Typing of Acine¬tobac-ter Spp. Strains |
| title_full | Antimicrobial Susceptibility and AP-PCR Typing of Acine¬tobac-ter Spp. Strains |
| title_fullStr | Antimicrobial Susceptibility and AP-PCR Typing of Acine¬tobac-ter Spp. Strains |
| title_full_unstemmed | Antimicrobial Susceptibility and AP-PCR Typing of Acine¬tobac-ter Spp. Strains |
| title_short | Antimicrobial Susceptibility and AP-PCR Typing of Acine¬tobac-ter Spp. Strains |
| title_sort | antimicrobial susceptibility and ap pcr typing of acine¬tobac ter spp strains |
| topic | Acinetobacter baumannii |
| url | http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/4255.pdf&manuscript_id=4255 |
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