The Canine POMC Gene, Obesity in Labrador Retrievers and Susceptibility to Diabetes Mellitus
Background Diabetes mellitus (DM) in dogs is a common endocrinopathy with a complex genetic architecture. Disease susceptibility in several breeds is associated with polymorphisms in immune response genes, but in the Labrador retriever breed, no genetic associations with DM have been identified. A d...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2017-03-01
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| Series: | Journal of Veterinary Internal Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1111/jvim.14636 |
| _version_ | 1811237820058042368 |
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| author | L.J. Davison A. Holder B. Catchpole C.A. O'Callaghan |
| author_facet | L.J. Davison A. Holder B. Catchpole C.A. O'Callaghan |
| author_sort | L.J. Davison |
| collection | DOAJ |
| description | Background Diabetes mellitus (DM) in dogs is a common endocrinopathy with a complex genetic architecture. Disease susceptibility in several breeds is associated with polymorphisms in immune response genes, but in the Labrador retriever breed, no genetic associations with DM have been identified. A deletion in the pro‐opiomelanocortin (POMC) gene in Labrador retrievers is associated with increased appetite and risk of obesity. Hypothesis/Objectives To characterize the POMC deletion in Labrador retrievers, to develop a simple genetic test for this mutation, and to test the hypothesis that the POMC gene deletion is associated with an increased risk of DM in this breed. Animals Sixty‐one non‐diabetic Labrador retrievers aged >6 years and 57 Labrador retrievers with DM. Methods Case–control genotyping study to compare the frequency of the POMC deletion in dogs with and without DM. After polymerase chain reaction (PCR) and sequencing to characterize the mutation, a PCR‐based test was developed and validated using 2 different restriction fragment length polymorphism assays. Results A 14‐base‐pair deletion was confirmed and localized to exon 3 of the canine POMC gene. A PCR‐based test for the deletion was successfully developed. There was no association between the presence of the POMC deletion mutation and DM in this population of Labrador retriever dogs (P = .31). Conclusions and Clinical Importance This study adds to the existing scientific literature indicating that there is little evidence for a direct link between obesity and DM in dogs. |
| first_indexed | 2024-04-12T12:29:48Z |
| format | Article |
| id | doaj.art-7836f26c8b6f47ad9f7096280b8f80bd |
| institution | Directory Open Access Journal |
| issn | 0891-6640 1939-1676 |
| language | English |
| last_indexed | 2024-04-12T12:29:48Z |
| publishDate | 2017-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Veterinary Internal Medicine |
| spelling | doaj.art-7836f26c8b6f47ad9f7096280b8f80bd2022-12-22T03:33:03ZengWileyJournal of Veterinary Internal Medicine0891-66401939-16762017-03-0131234334810.1111/jvim.14636The Canine POMC Gene, Obesity in Labrador Retrievers and Susceptibility to Diabetes MellitusL.J. Davison0A. Holder1B. Catchpole2C.A. O'Callaghan3Department of Veterinary Medicine Queen's Veterinary School Hospital University of Cambridge Cambridge UKDepartment of Pathology and Pathogen Biology Royal Veterinary College Hatfield UKDepartment of Pathology and Pathogen Biology Royal Veterinary College Hatfield UKNuffield Department of Medicine Wellcome Trust Centre for Human Genetics University of Oxford Oxford UKBackground Diabetes mellitus (DM) in dogs is a common endocrinopathy with a complex genetic architecture. Disease susceptibility in several breeds is associated with polymorphisms in immune response genes, but in the Labrador retriever breed, no genetic associations with DM have been identified. A deletion in the pro‐opiomelanocortin (POMC) gene in Labrador retrievers is associated with increased appetite and risk of obesity. Hypothesis/Objectives To characterize the POMC deletion in Labrador retrievers, to develop a simple genetic test for this mutation, and to test the hypothesis that the POMC gene deletion is associated with an increased risk of DM in this breed. Animals Sixty‐one non‐diabetic Labrador retrievers aged >6 years and 57 Labrador retrievers with DM. Methods Case–control genotyping study to compare the frequency of the POMC deletion in dogs with and without DM. After polymerase chain reaction (PCR) and sequencing to characterize the mutation, a PCR‐based test was developed and validated using 2 different restriction fragment length polymorphism assays. Results A 14‐base‐pair deletion was confirmed and localized to exon 3 of the canine POMC gene. A PCR‐based test for the deletion was successfully developed. There was no association between the presence of the POMC deletion mutation and DM in this population of Labrador retriever dogs (P = .31). Conclusions and Clinical Importance This study adds to the existing scientific literature indicating that there is little evidence for a direct link between obesity and DM in dogs.https://doi.org/10.1111/jvim.14636GeneticsEndocrinologyPolymerase chain reactionPancreas |
| spellingShingle | L.J. Davison A. Holder B. Catchpole C.A. O'Callaghan The Canine POMC Gene, Obesity in Labrador Retrievers and Susceptibility to Diabetes Mellitus Journal of Veterinary Internal Medicine Genetics Endocrinology Polymerase chain reaction Pancreas |
| title | The Canine POMC Gene, Obesity in Labrador Retrievers and Susceptibility to Diabetes Mellitus |
| title_full | The Canine POMC Gene, Obesity in Labrador Retrievers and Susceptibility to Diabetes Mellitus |
| title_fullStr | The Canine POMC Gene, Obesity in Labrador Retrievers and Susceptibility to Diabetes Mellitus |
| title_full_unstemmed | The Canine POMC Gene, Obesity in Labrador Retrievers and Susceptibility to Diabetes Mellitus |
| title_short | The Canine POMC Gene, Obesity in Labrador Retrievers and Susceptibility to Diabetes Mellitus |
| title_sort | canine pomc gene obesity in labrador retrievers and susceptibility to diabetes mellitus |
| topic | Genetics Endocrinology Polymerase chain reaction Pancreas |
| url | https://doi.org/10.1111/jvim.14636 |
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