Immunohystochemical study of the largest islets of human pancreas in aging and diabetes mellitus: perspectives for the transplantation
Aim. To study the distribution and cellular architecture of the largest human pancreatic islets (with a diameter of more than 200 micron) in aging and diabetes mellitus types 1 and 2. Materials and methods. Antibodies to insulin, glucagon, somastatin and nturon-specific enolase were applied. The...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Endocrinology Research Centre
2013-12-01
|
| Series: | Сахарный диабет |
| Subjects: | |
| Online Access: | https://www.dia-endojournals.ru/jour/article/view/6270 |
| _version_ | 1826588115366576128 |
|---|---|
| author | Alexandra Evgen'evna Proshchina Yulia Sergeevna Krivova Valeriy Mikhaylovich Barabanov Sergey Vyacheslavovich Savelyev |
| author_facet | Alexandra Evgen'evna Proshchina Yulia Sergeevna Krivova Valeriy Mikhaylovich Barabanov Sergey Vyacheslavovich Savelyev |
| author_sort | Alexandra Evgen'evna Proshchina |
| collection | DOAJ |
| description | Aim. To study the distribution and cellular architecture of the largest human pancreatic islets (with a diameter of more than 200 micron) in aging and diabetes mellitus types 1 and 2. Materials and methods. Antibodies to insulin, glucagon, somastatin and nturon-specific enolase were applied. The autopsy samples of the pancreatic tissue of patients with diabetes mellitus type 1 (DMT1) and type 2 (DMT2) and 2 age groups (up to 50 years old (control) and after 50 (aging control)), not suffering from diseases of the pancreas and carbohydrate metabolism malfunction were investigated. Results. The number of islets with diameter more than 200 mkm compared to control group increased both in aging and diabetes groups. Their number reaches in some cases 15% (and higher in DM) of the total number of islets. These islets compared to the other are rich-vascularized. It was shown that glucagon and somatostatin-containing cells are found both on the periphery of the large islets, and inside them only in the immediate proximity of the capillaries. Insulin-containing cells form clusters, surrounded by the capillaries and the ?- and ?-cells, while the inner part of such clusters has no direct contact with the capillaries. In the large islets the number of glucagon-containing cells is often increased, and insulin-containing cells show signs of degradation. Conclusion. The largest of the pancreatic islets may be useless for the transplantation, because of the high content of glucagon-containing cells, the rich vascularization and, in some cases, the limited functionality of ?-cells. |
| first_indexed | 2024-03-08T15:21:23Z |
| format | Article |
| id | doaj.art-783a57cd494e4763838ae2a1242a6119 |
| institution | Directory Open Access Journal |
| issn | 2072-0351 2072-0378 |
| language | English |
| last_indexed | 2025-03-14T16:36:12Z |
| publishDate | 2013-12-01 |
| publisher | Endocrinology Research Centre |
| record_format | Article |
| series | Сахарный диабет |
| spelling | doaj.art-783a57cd494e4763838ae2a1242a61192025-02-21T09:29:32ZengEndocrinology Research CentreСахарный диабет2072-03512072-03782013-12-01164384310.14341/DM2013438-436217Immunohystochemical study of the largest islets of human pancreas in aging and diabetes mellitus: perspectives for the transplantationAlexandra Evgen'evna Proshchina0Yulia Sergeevna Krivova1Valeriy Mikhaylovich Barabanov2Sergey Vyacheslavovich Savelyev3Research Institute of Human Morphology of the Russian Academy of Medical SciencesResearch Institute of Human Morphology of the Russian Academy of Medical SciencesResearch Institute of Human Morphology of the Russian Academy of Medical SciencesResearch Institute of Human Morphology of the Russian Academy of Medical SciencesAim. To study the distribution and cellular architecture of the largest human pancreatic islets (with a diameter of more than 200 micron) in aging and diabetes mellitus types 1 and 2. Materials and methods. Antibodies to insulin, glucagon, somastatin and nturon-specific enolase were applied. The autopsy samples of the pancreatic tissue of patients with diabetes mellitus type 1 (DMT1) and type 2 (DMT2) and 2 age groups (up to 50 years old (control) and after 50 (aging control)), not suffering from diseases of the pancreas and carbohydrate metabolism malfunction were investigated. Results. The number of islets with diameter more than 200 mkm compared to control group increased both in aging and diabetes groups. Their number reaches in some cases 15% (and higher in DM) of the total number of islets. These islets compared to the other are rich-vascularized. It was shown that glucagon and somatostatin-containing cells are found both on the periphery of the large islets, and inside them only in the immediate proximity of the capillaries. Insulin-containing cells form clusters, surrounded by the capillaries and the ?- and ?-cells, while the inner part of such clusters has no direct contact with the capillaries. In the large islets the number of glucagon-containing cells is often increased, and insulin-containing cells show signs of degradation. Conclusion. The largest of the pancreatic islets may be useless for the transplantation, because of the high content of glucagon-containing cells, the rich vascularization and, in some cases, the limited functionality of ?-cells.https://www.dia-endojournals.ru/jour/article/view/6270isletsdiabetes mellitusaginginsulinglucagonsomatostatinneuron-specific enolasensetransplantation |
| spellingShingle | Alexandra Evgen'evna Proshchina Yulia Sergeevna Krivova Valeriy Mikhaylovich Barabanov Sergey Vyacheslavovich Savelyev Immunohystochemical study of the largest islets of human pancreas in aging and diabetes mellitus: perspectives for the transplantation Сахарный диабет islets diabetes mellitus aging insulin glucagon somatostatin neuron-specific enolase nse transplantation |
| title | Immunohystochemical study of the largest islets of human pancreas in aging and diabetes mellitus: perspectives for the transplantation |
| title_full | Immunohystochemical study of the largest islets of human pancreas in aging and diabetes mellitus: perspectives for the transplantation |
| title_fullStr | Immunohystochemical study of the largest islets of human pancreas in aging and diabetes mellitus: perspectives for the transplantation |
| title_full_unstemmed | Immunohystochemical study of the largest islets of human pancreas in aging and diabetes mellitus: perspectives for the transplantation |
| title_short | Immunohystochemical study of the largest islets of human pancreas in aging and diabetes mellitus: perspectives for the transplantation |
| title_sort | immunohystochemical study of the largest islets of human pancreas in aging and diabetes mellitus perspectives for the transplantation |
| topic | islets diabetes mellitus aging insulin glucagon somatostatin neuron-specific enolase nse transplantation |
| url | https://www.dia-endojournals.ru/jour/article/view/6270 |
| work_keys_str_mv | AT alexandraevgenevnaproshchina immunohystochemicalstudyofthelargestisletsofhumanpancreasinaginganddiabetesmellitusperspectivesforthetransplantation AT yuliasergeevnakrivova immunohystochemicalstudyofthelargestisletsofhumanpancreasinaginganddiabetesmellitusperspectivesforthetransplantation AT valeriymikhaylovichbarabanov immunohystochemicalstudyofthelargestisletsofhumanpancreasinaginganddiabetesmellitusperspectivesforthetransplantation AT sergeyvyacheslavovichsavelyev immunohystochemicalstudyofthelargestisletsofhumanpancreasinaginganddiabetesmellitusperspectivesforthetransplantation |