Stable Nitrogen Isotopes as an Effective Tool for Estimating the Nitrogen Demand of <i>Broussonetia papyrifera</i> (L.) Vent Seedlings under Variable Nitrate Concentrations
Poor growth is often observed in artificial young forests due to insufficient inorganic nitrogen in karst soils. However, little is known about the assimilatory demand of the whole plant for nitrate and the partitioning of nitrate assimilation in roots and leaves in woody plants grown in karst habit...
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MDPI AG
2023-06-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/13/6/1663 |
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| author | Kaiyan Zhang Furong Zhang Haitao Li Yue Su Yanyou Wu |
| author_facet | Kaiyan Zhang Furong Zhang Haitao Li Yue Su Yanyou Wu |
| author_sort | Kaiyan Zhang |
| collection | DOAJ |
| description | Poor growth is often observed in artificial young forests due to insufficient inorganic nitrogen in karst soils. However, little is known about the assimilatory demand of the whole plant for nitrate and the partitioning of nitrate assimilation in roots and leaves in woody plants grown in karst habitats. In this study, <i>Broussonetia papyrifera</i> (L.) Vent (<i>B. papyrifera</i>) seedlings were grown under nearly hydroponic conditions. The isotope mass balance approach was employed to quantify the δ<sup>15</sup>N values of the N assimilates in plant organs and in whole plants for <i>B. papyrifera</i> seedlings grown at different nitrate concentrations. The δ<sup>15</sup>N values of the N assimilates in the whole <i>B. papyrifera</i> seedlings showed a rising trend with increasing nitrate concentration. Increasing the supply of nitrate decreased the leaf–root difference in the δ<sup>15</sup>N values of the N assimilates for <i>B. papyrifera</i> seedlings. Quantifying the δ<sup>15</sup>N values of N assimilates in the whole <i>B. papyrifera</i> seedlings grown under different nitrate concentrations contributes to estimating the assimilatory demand of the <i>B. papyrifera</i> seedlings for nitrate. The leaf–root difference in the δ<sup>15</sup>N values of the N assimilates can be used to estimate the partitioning of nitrate assimilation in the roots and leaves. |
| first_indexed | 2024-03-11T02:51:49Z |
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| id | doaj.art-783d5e8810104c96aa7110ebea131a0d |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-11T02:51:49Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-783d5e8810104c96aa7110ebea131a0d2023-11-18T08:56:20ZengMDPI AGAgronomy2073-43952023-06-01136166310.3390/agronomy13061663Stable Nitrogen Isotopes as an Effective Tool for Estimating the Nitrogen Demand of <i>Broussonetia papyrifera</i> (L.) Vent Seedlings under Variable Nitrate ConcentrationsKaiyan Zhang0Furong Zhang1Haitao Li2Yue Su3Yanyou Wu4School of Karst Science, Guizhou Normal University/State Engineering Technology Institute for Karst Desertification Control, Guiyang 550001, ChinaSchool of Agricultural Engineering, Jiangsu University, Zhenjiang 212013, ChinaEngineering Technology Research Center for Protection and Detection of Germplasm Resources of Karst-Adaptable Crops, Guizhou Vocational College of Agriculture, Qingzhen 551400, ChinaEngineering Technology Research Center for Protection and Detection of Germplasm Resources of Karst-Adaptable Crops, Guizhou Vocational College of Agriculture, Qingzhen 551400, ChinaState Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang 550081, ChinaPoor growth is often observed in artificial young forests due to insufficient inorganic nitrogen in karst soils. However, little is known about the assimilatory demand of the whole plant for nitrate and the partitioning of nitrate assimilation in roots and leaves in woody plants grown in karst habitats. In this study, <i>Broussonetia papyrifera</i> (L.) Vent (<i>B. papyrifera</i>) seedlings were grown under nearly hydroponic conditions. The isotope mass balance approach was employed to quantify the δ<sup>15</sup>N values of the N assimilates in plant organs and in whole plants for <i>B. papyrifera</i> seedlings grown at different nitrate concentrations. The δ<sup>15</sup>N values of the N assimilates in the whole <i>B. papyrifera</i> seedlings showed a rising trend with increasing nitrate concentration. Increasing the supply of nitrate decreased the leaf–root difference in the δ<sup>15</sup>N values of the N assimilates for <i>B. papyrifera</i> seedlings. Quantifying the δ<sup>15</sup>N values of N assimilates in the whole <i>B. papyrifera</i> seedlings grown under different nitrate concentrations contributes to estimating the assimilatory demand of the <i>B. papyrifera</i> seedlings for nitrate. The leaf–root difference in the δ<sup>15</sup>N values of the N assimilates can be used to estimate the partitioning of nitrate assimilation in the roots and leaves.https://www.mdpi.com/2073-4395/13/6/1663karstartificial forestnitrateisotope mass balance approachassimilatory demand |
| spellingShingle | Kaiyan Zhang Furong Zhang Haitao Li Yue Su Yanyou Wu Stable Nitrogen Isotopes as an Effective Tool for Estimating the Nitrogen Demand of <i>Broussonetia papyrifera</i> (L.) Vent Seedlings under Variable Nitrate Concentrations Agronomy karst artificial forest nitrate isotope mass balance approach assimilatory demand |
| title | Stable Nitrogen Isotopes as an Effective Tool for Estimating the Nitrogen Demand of <i>Broussonetia papyrifera</i> (L.) Vent Seedlings under Variable Nitrate Concentrations |
| title_full | Stable Nitrogen Isotopes as an Effective Tool for Estimating the Nitrogen Demand of <i>Broussonetia papyrifera</i> (L.) Vent Seedlings under Variable Nitrate Concentrations |
| title_fullStr | Stable Nitrogen Isotopes as an Effective Tool for Estimating the Nitrogen Demand of <i>Broussonetia papyrifera</i> (L.) Vent Seedlings under Variable Nitrate Concentrations |
| title_full_unstemmed | Stable Nitrogen Isotopes as an Effective Tool for Estimating the Nitrogen Demand of <i>Broussonetia papyrifera</i> (L.) Vent Seedlings under Variable Nitrate Concentrations |
| title_short | Stable Nitrogen Isotopes as an Effective Tool for Estimating the Nitrogen Demand of <i>Broussonetia papyrifera</i> (L.) Vent Seedlings under Variable Nitrate Concentrations |
| title_sort | stable nitrogen isotopes as an effective tool for estimating the nitrogen demand of i broussonetia papyrifera i l vent seedlings under variable nitrate concentrations |
| topic | karst artificial forest nitrate isotope mass balance approach assimilatory demand |
| url | https://www.mdpi.com/2073-4395/13/6/1663 |
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