Research Note: Characterization of S-Meq containing the deletion in Meq protein's transactivation domain in a Marek's disease virus strain in Japan
ABSTRACT: Marek's disease virus (MDV) causes malignant lymphomas in chickens (Marek's disease; MD). Although MD has caused significant economic losses to the poultry industry, currently, its occurrence in the field is effectively controlled by vaccination. However, the genetic characterist...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-11-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579121004843 |
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| author | Shiro Murata Eiji Yamamoto Natsumi Sakashita Naoya Maekawa Tomohiro Okagawa Satoru Konnai Kazuhiko Ohashi |
| author_facet | Shiro Murata Eiji Yamamoto Natsumi Sakashita Naoya Maekawa Tomohiro Okagawa Satoru Konnai Kazuhiko Ohashi |
| author_sort | Shiro Murata |
| collection | DOAJ |
| description | ABSTRACT: Marek's disease virus (MDV) causes malignant lymphomas in chickens (Marek's disease; MD). Although MD has caused significant economic losses to the poultry industry, currently, its occurrence in the field is effectively controlled by vaccination. However, the genetic characteristics of MDV strains have changed, and the poultry industry has experienced MD outbreaks in vaccinated chickens because of enhanced virulence. Meq, an oncoprotein of MDV, is a key transcription factor correlated with the tumorigenesis in MD. Animal experiments using recombinant MDV revealed that distinct polymorphisms in Meq affect the virulence of MDV strains. Meq containing an insertion or deletion is present in some MDV strains. In the 2010s, field strains that encode Meq containing the deletion (S-Meq) were reported. In this study, we characterized the genetic features of S-Meq detected in a Japanese MDV strain and analyzed its transactivation activity to investigate S-Meq's protein function. S-Meq lacked 41 amino acids, and the deletion was at the same position as those observed in other countries. In addition, S-Meq exhibited higher transactivation activity than Meq from other MDV strains circulating in Japan. These results suggest that the deletion in the transactivation domain may enhance the Meq protein's function. Further investigation is needed to clarify whether the deletion in the transactivation domain of Meq affects MDV's virulence. |
| first_indexed | 2024-12-20T19:06:52Z |
| format | Article |
| id | doaj.art-783fc3c652cd4794b31df61c0453f155 |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-12-20T19:06:52Z |
| publishDate | 2021-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-783fc3c652cd4794b31df61c0453f1552022-12-21T19:29:16ZengElsevierPoultry Science0032-57912021-11-0110011101461Research Note: Characterization of S-Meq containing the deletion in Meq protein's transactivation domain in a Marek's disease virus strain in JapanShiro Murata0Eiji Yamamoto1Natsumi Sakashita2Naoya Maekawa3Tomohiro Okagawa4Satoru Konnai5Kazuhiko Ohashi6Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan; Department of Advanced Pharmaceutics, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan; Corresponding author:Western Livestock Hygiene Service Center, Kagawa Prefectural Government, Zentsuji, JapanLivestock Division, Kagawa Prefectural Government, Takamatsu, JapanDepartment of Advanced Pharmaceutics, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, JapanDepartment of Advanced Pharmaceutics, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, JapanDepartment of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan; Department of Advanced Pharmaceutics, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, JapanDepartment of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan; Department of Advanced Pharmaceutics, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, JapanABSTRACT: Marek's disease virus (MDV) causes malignant lymphomas in chickens (Marek's disease; MD). Although MD has caused significant economic losses to the poultry industry, currently, its occurrence in the field is effectively controlled by vaccination. However, the genetic characteristics of MDV strains have changed, and the poultry industry has experienced MD outbreaks in vaccinated chickens because of enhanced virulence. Meq, an oncoprotein of MDV, is a key transcription factor correlated with the tumorigenesis in MD. Animal experiments using recombinant MDV revealed that distinct polymorphisms in Meq affect the virulence of MDV strains. Meq containing an insertion or deletion is present in some MDV strains. In the 2010s, field strains that encode Meq containing the deletion (S-Meq) were reported. In this study, we characterized the genetic features of S-Meq detected in a Japanese MDV strain and analyzed its transactivation activity to investigate S-Meq's protein function. S-Meq lacked 41 amino acids, and the deletion was at the same position as those observed in other countries. In addition, S-Meq exhibited higher transactivation activity than Meq from other MDV strains circulating in Japan. These results suggest that the deletion in the transactivation domain may enhance the Meq protein's function. Further investigation is needed to clarify whether the deletion in the transactivation domain of Meq affects MDV's virulence.http://www.sciencedirect.com/science/article/pii/S0032579121004843deletionMarek's disease virusMeqS-Meqtransactivation |
| spellingShingle | Shiro Murata Eiji Yamamoto Natsumi Sakashita Naoya Maekawa Tomohiro Okagawa Satoru Konnai Kazuhiko Ohashi Research Note: Characterization of S-Meq containing the deletion in Meq protein's transactivation domain in a Marek's disease virus strain in Japan Poultry Science deletion Marek's disease virus Meq S-Meq transactivation |
| title | Research Note: Characterization of S-Meq containing the deletion in Meq protein's transactivation domain in a Marek's disease virus strain in Japan |
| title_full | Research Note: Characterization of S-Meq containing the deletion in Meq protein's transactivation domain in a Marek's disease virus strain in Japan |
| title_fullStr | Research Note: Characterization of S-Meq containing the deletion in Meq protein's transactivation domain in a Marek's disease virus strain in Japan |
| title_full_unstemmed | Research Note: Characterization of S-Meq containing the deletion in Meq protein's transactivation domain in a Marek's disease virus strain in Japan |
| title_short | Research Note: Characterization of S-Meq containing the deletion in Meq protein's transactivation domain in a Marek's disease virus strain in Japan |
| title_sort | research note characterization of s meq containing the deletion in meq protein s transactivation domain in a marek s disease virus strain in japan |
| topic | deletion Marek's disease virus Meq S-Meq transactivation |
| url | http://www.sciencedirect.com/science/article/pii/S0032579121004843 |
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