Electron microscopy structure of collagen-based corneal substitutes
Purpose: To investigate the electron microscopy structure of biosynthetic corneal substitutes (CS) based on interpenetrating networks of collagen and 2-methacryloyloxyethyl phosphorylcholine (C-MPC). Materials and Methods: Transmission electron microscopy was used to investigate the structure of...
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| Format: | Article |
| Language: | English |
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Ukrainian Society of Ophthalmologists
2017-08-01
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| Series: | Journal of Ophthalmology |
| Subjects: | |
| Online Access: | https://www.ozhurnal.com/en/archive/2017/4/7-fulltext |
| _version_ | 1797357439914344448 |
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| author | N.I. Molchaniuk O.I. Buznyk N.E. Dumbrova N.V. Pasyechnikova |
| author_facet | N.I. Molchaniuk O.I. Buznyk N.E. Dumbrova N.V. Pasyechnikova |
| author_sort | N.I. Molchaniuk |
| collection | DOAJ |
| description | Purpose: To investigate the electron microscopy structure of biosynthetic corneal substitutes (CS) based on interpenetrating networks of collagen and 2-methacryloyloxyethyl phosphorylcholine (C-MPC).
Materials and Methods: Transmission electron microscopy was used to investigate the structure of C-MPC CS made from 18% solution of recombinant human collagen (RHC) type III or porcine collagen (PCOL) type I.
Results: Narrow (2-8 nm) collagen-like fibrils with a regular, mainly longitudinal orientation were found in the corneal substitutes. Although the ultrastructure and arrangement of these fibrils were typical for those of human corneal structure, they did not demonstrate some features of the human corneal structure. The fibrils from PCOL-MPC CS were somewhat wider and less regularly arranged than those from RHC-MPC CS; however, this did not effect the transparency of the implants.
Conclusion: PCOL-MPC implants and RHC-MPC implants have as good optical properties as the human cornea, but their structure and arrangement of filaments are different from those of the human cornea, which may be associated with a very small size of the fibrils produced as a result of collagen molecule cross-linking; that is why they do not scatter light. |
| first_indexed | 2024-03-08T14:45:03Z |
| format | Article |
| id | doaj.art-7840ade55aaf4692a28a5dd419c7ffb1 |
| institution | Directory Open Access Journal |
| issn | 2412-8740 |
| language | English |
| last_indexed | 2024-03-08T14:45:03Z |
| publishDate | 2017-08-01 |
| publisher | Ukrainian Society of Ophthalmologists |
| record_format | Article |
| series | Journal of Ophthalmology |
| spelling | doaj.art-7840ade55aaf4692a28a5dd419c7ffb12024-01-11T12:16:14ZengUkrainian Society of OphthalmologistsJournal of Ophthalmology2412-87402017-08-014404310.31288/oftalmolzh201744043Electron microscopy structure of collagen-based corneal substitutesN.I. Molchaniuk0O.I. Buznyk1N.E. Dumbrova2N.V. Pasyechnikova3Filatov Institute of Eye Diseases and Tissue TherapyFilatov Institute of Eye Diseases and Tissue TherapyFilatov Institute of Eye Diseases and Tissue TherapyFilatov Institute of Eye Diseases and Tissue TherapyPurpose: To investigate the electron microscopy structure of biosynthetic corneal substitutes (CS) based on interpenetrating networks of collagen and 2-methacryloyloxyethyl phosphorylcholine (C-MPC). Materials and Methods: Transmission electron microscopy was used to investigate the structure of C-MPC CS made from 18% solution of recombinant human collagen (RHC) type III or porcine collagen (PCOL) type I. Results: Narrow (2-8 nm) collagen-like fibrils with a regular, mainly longitudinal orientation were found in the corneal substitutes. Although the ultrastructure and arrangement of these fibrils were typical for those of human corneal structure, they did not demonstrate some features of the human corneal structure. The fibrils from PCOL-MPC CS were somewhat wider and less regularly arranged than those from RHC-MPC CS; however, this did not effect the transparency of the implants. Conclusion: PCOL-MPC implants and RHC-MPC implants have as good optical properties as the human cornea, but their structure and arrangement of filaments are different from those of the human cornea, which may be associated with a very small size of the fibrils produced as a result of collagen molecule cross-linking; that is why they do not scatter light.https://www.ozhurnal.com/en/archive/2017/4/7-fulltextcorneal substitutedonor cornea substitutecollagenelectron microscopy structure |
| spellingShingle | N.I. Molchaniuk O.I. Buznyk N.E. Dumbrova N.V. Pasyechnikova Electron microscopy structure of collagen-based corneal substitutes Journal of Ophthalmology corneal substitute donor cornea substitute collagen electron microscopy structure |
| title | Electron microscopy structure of collagen-based corneal substitutes |
| title_full | Electron microscopy structure of collagen-based corneal substitutes |
| title_fullStr | Electron microscopy structure of collagen-based corneal substitutes |
| title_full_unstemmed | Electron microscopy structure of collagen-based corneal substitutes |
| title_short | Electron microscopy structure of collagen-based corneal substitutes |
| title_sort | electron microscopy structure of collagen based corneal substitutes |
| topic | corneal substitute donor cornea substitute collagen electron microscopy structure |
| url | https://www.ozhurnal.com/en/archive/2017/4/7-fulltext |
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