Production of recombinant CAMP – Sialidase protein and preparation of chitosan nanoparticles carrying this protein to be used as a candidate for vaccines targeting Propionibacterium acnes
BACKGROUND AND OBJECTIVE: Acne vulgaris is one of the most common skin diseases that imposes too much mental pressure and high costs on patients. The existing treatments have low efficacy and include antibiotics and anti-inflammatory drugs, which have many complications due to the chronic nature of...
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Language: | English |
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Babol University of Medical Sciences
2018-02-01
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Series: | Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul |
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Online Access: | http://jbums.org/browse.php?a_code=A-10-3435-1&slc_lang=en&sid=1 |
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author | P Zargham M Heshmati K Mansouri J Amani J Salimian A Ahmadi |
author_facet | P Zargham M Heshmati K Mansouri J Amani J Salimian A Ahmadi |
author_sort | P Zargham |
collection | DOAJ |
description | BACKGROUND AND OBJECTIVE: Acne vulgaris is one of the most common skin diseases that imposes too much mental pressure and high costs on patients. The existing treatments have low efficacy and include antibiotics and anti-inflammatory drugs, which have many complications due to the chronic nature of the disease, especially at young age, including antibiotic resistance and allergic reactions. For this reason, one of the new therapeutic approaches is the use of a vaccine with the help of the bacterium or its components. The aim of this study is to produce nanoparticles carrying the recombinant CAMP – Sialidase protein as a new chimeric antigen to be used in acne vaccine.
METHODS: To express the recombinant CAMP – Sialidase protein, E. coli BL21 DE3 was used as the host. Purification of protein was done through combined urea/imidazole method and using a nickel-nitroacetic acid column. The recombinant protein was confirmed using Western Blotting by Anti – Histidine Antibody. Then, the loaded nanoparticles were prepared by recombinant protein using ionic gelation technique and tripolyphosphate. Finally, the size and zeta potential of the nanoparticles were determined by the DLS device.
FINDINGS: The recombinant CAMP – Sialidase protein was confirmed after expression and purification. The size and zeta potential of nanoparticles containing recombinant CAMP – Sialidase protein at a concentration of 0.6 mg / ml were determined to be 80 nm and +27 mV, respectively, using the DLS device. The loading rate of the protein in the nanoparticles was found to be 88%.
CONCLUSION: The results show that the recombinant protein is expressed completely and is successfully encapsulated in the chitosan nanoparticles. |
first_indexed | 2024-04-13T14:40:40Z |
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institution | Directory Open Access Journal |
issn | 1561-4107 2251-7170 |
language | English |
last_indexed | 2024-04-13T14:40:40Z |
publishDate | 2018-02-01 |
publisher | Babol University of Medical Sciences |
record_format | Article |
series | Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul |
spelling | doaj.art-7843efd238c6432db17947e6b13c98e32022-12-22T02:42:54ZengBabol University of Medical SciencesMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul1561-41072251-71702018-02-012026469Production of recombinant CAMP – Sialidase protein and preparation of chitosan nanoparticles carrying this protein to be used as a candidate for vaccines targeting Propionibacterium acnesP Zargham0M Heshmati1K Mansouri2J Amani3J Salimian4A Ahmadi5 1. Department of Molecular and Cellular Sciences, Faculty of Advanced Sciences & Technology, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, I.R.Iran. 1. Department of Molecular and Cellular Sciences, Faculty of Advanced Sciences & Technology, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, I.R.Iran. 2. Chemical Injuries Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, I.R.Iran. 3. Applied Microbiology Research Center, Institute of Biology and Food Poisoning, Baqiyatallah University of Medical Sciences, Tehran, I.R.Iran. 2. Chemical Injuries Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, I.R.Iran. 4. Molecular Biology Research Center, Institute of Biology and Food Poisoning, Baqiyatallah University of Medical Sciences, Tehran, I.R.Iran. BACKGROUND AND OBJECTIVE: Acne vulgaris is one of the most common skin diseases that imposes too much mental pressure and high costs on patients. The existing treatments have low efficacy and include antibiotics and anti-inflammatory drugs, which have many complications due to the chronic nature of the disease, especially at young age, including antibiotic resistance and allergic reactions. For this reason, one of the new therapeutic approaches is the use of a vaccine with the help of the bacterium or its components. The aim of this study is to produce nanoparticles carrying the recombinant CAMP – Sialidase protein as a new chimeric antigen to be used in acne vaccine. METHODS: To express the recombinant CAMP – Sialidase protein, E. coli BL21 DE3 was used as the host. Purification of protein was done through combined urea/imidazole method and using a nickel-nitroacetic acid column. The recombinant protein was confirmed using Western Blotting by Anti – Histidine Antibody. Then, the loaded nanoparticles were prepared by recombinant protein using ionic gelation technique and tripolyphosphate. Finally, the size and zeta potential of the nanoparticles were determined by the DLS device. FINDINGS: The recombinant CAMP – Sialidase protein was confirmed after expression and purification. The size and zeta potential of nanoparticles containing recombinant CAMP – Sialidase protein at a concentration of 0.6 mg / ml were determined to be 80 nm and +27 mV, respectively, using the DLS device. The loading rate of the protein in the nanoparticles was found to be 88%. CONCLUSION: The results show that the recombinant protein is expressed completely and is successfully encapsulated in the chitosan nanoparticles.http://jbums.org/browse.php?a_code=A-10-3435-1&slc_lang=en&sid=1Acne VulgarisCamp – Sialidase ProteinChitosan NanoparticleVaccine |
spellingShingle | P Zargham M Heshmati K Mansouri J Amani J Salimian A Ahmadi Production of recombinant CAMP – Sialidase protein and preparation of chitosan nanoparticles carrying this protein to be used as a candidate for vaccines targeting Propionibacterium acnes Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul Acne Vulgaris Camp – Sialidase Protein Chitosan Nanoparticle Vaccine |
title | Production of recombinant CAMP – Sialidase protein and preparation of chitosan nanoparticles carrying this protein to be used as a candidate for vaccines targeting Propionibacterium acnes |
title_full | Production of recombinant CAMP – Sialidase protein and preparation of chitosan nanoparticles carrying this protein to be used as a candidate for vaccines targeting Propionibacterium acnes |
title_fullStr | Production of recombinant CAMP – Sialidase protein and preparation of chitosan nanoparticles carrying this protein to be used as a candidate for vaccines targeting Propionibacterium acnes |
title_full_unstemmed | Production of recombinant CAMP – Sialidase protein and preparation of chitosan nanoparticles carrying this protein to be used as a candidate for vaccines targeting Propionibacterium acnes |
title_short | Production of recombinant CAMP – Sialidase protein and preparation of chitosan nanoparticles carrying this protein to be used as a candidate for vaccines targeting Propionibacterium acnes |
title_sort | production of recombinant camp sialidase protein and preparation of chitosan nanoparticles carrying this protein to be used as a candidate for vaccines targeting propionibacterium acnes |
topic | Acne Vulgaris Camp – Sialidase Protein Chitosan Nanoparticle Vaccine |
url | http://jbums.org/browse.php?a_code=A-10-3435-1&slc_lang=en&sid=1 |
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