Repair of osteochondral defects mediated by double-layer scaffolds with natural osteochondral-biomimetic microenvironment and interface
Tissue engineering provides a new approach for the treatment of osteochondral defects. However, the lack of an ideal double-layer scaffold with osteochondral-biomimetic microenvironment and interface similar to native articular tissue greatly limits clinical translation. Our current study developed...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-03-01
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| Series: | Materials Today Bio |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006422000321 |
| _version_ | 1811244512403521536 |
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| author | Tao Wang Wei Xu Xintong Zhao Baoshuai Bai Yuejie Hua Jincheng Tang Feifan Chen Yu Liu Yahui Wang Guangdong Zhou Yilin Cao |
| author_facet | Tao Wang Wei Xu Xintong Zhao Baoshuai Bai Yuejie Hua Jincheng Tang Feifan Chen Yu Liu Yahui Wang Guangdong Zhou Yilin Cao |
| author_sort | Tao Wang |
| collection | DOAJ |
| description | Tissue engineering provides a new approach for the treatment of osteochondral defects. However, the lack of an ideal double-layer scaffold with osteochondral-biomimetic microenvironment and interface similar to native articular tissue greatly limits clinical translation. Our current study developed a double-layer acellular osteochondral matrix (AOM) scaffold with natural osteochondral-biomimetic microenvironment and interface by integrating ultraviolet (UV) laser and decellularization techniques. The laser parameters were optimized to achieve a proper pore depth close to the osteochondral interface, which guaranteed complete decellularization, sufficient space for cell loading, and relative independence of the chondrogenic and osteogenic microenvironments. Gelatin-methacryloyl (GelMA) hydrogel was further used as the cell carrier to significantly enhance the efficiency and homogeneity of cell loading in the AOM scaffold with large pore structure. Additionally, in vitro results demonstrated that the components of the AOM scaffold could efficiently regulate the chondrogenic/osteogenic differentiations of bone marrow stromal cells (BMSCs) by activating the chondrogenic/osteogenic related pathways. Importantly, the AOM scaffolds combined with BMSC-laden GelMA hydrogel successfully realized tissue-specific repair of the osteochondral defects in a knee joint model of rabbit. The current study developed a novel double-layer osteochondral biomimetic scaffold and feasible strategy, providing strong support for the tissue-specific repair of osteochondral defects and its future clinical translation. |
| first_indexed | 2024-04-12T14:26:22Z |
| format | Article |
| id | doaj.art-7844102fce7040dc9748e680c311b1eb |
| institution | Directory Open Access Journal |
| issn | 2590-0064 |
| language | English |
| last_indexed | 2024-04-12T14:26:22Z |
| publishDate | 2022-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj.art-7844102fce7040dc9748e680c311b1eb2022-12-22T03:29:26ZengElsevierMaterials Today Bio2590-00642022-03-0114100234Repair of osteochondral defects mediated by double-layer scaffolds with natural osteochondral-biomimetic microenvironment and interfaceTao Wang0Wei Xu1Xintong Zhao2Baoshuai Bai3Yuejie Hua4Jincheng Tang5Feifan Chen6Yu Liu7Yahui Wang8Guangdong Zhou9Yilin Cao10Research Institute of Plastic Surgery, Wei Fang Medical University, Wei Fang, Shandong, 261041, PR China; National Tissue Engineering Center of China, Shanghai, 200241, PR ChinaResearch Institute of Plastic Surgery, Wei Fang Medical University, Wei Fang, Shandong, 261041, PR China; National Tissue Engineering Center of China, Shanghai, 200241, PR ChinaDepartment of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering, Shanghai, 200011, PR China; National Tissue Engineering Center of China, Shanghai, 200241, PR ChinaResearch Institute of Plastic Surgery, Wei Fang Medical University, Wei Fang, Shandong, 261041, PR China; National Tissue Engineering Center of China, Shanghai, 200241, PR ChinaDepartment of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering, Shanghai, 200011, PR China; National Tissue Engineering Center of China, Shanghai, 200241, PR ChinaResearch Institute of Plastic Surgery, Wei Fang Medical University, Wei Fang, Shandong, 261041, PR China; National Tissue Engineering Center of China, Shanghai, 200241, PR ChinaResearch Institute of Plastic Surgery, Wei Fang Medical University, Wei Fang, Shandong, 261041, PR China; National Tissue Engineering Center of China, Shanghai, 200241, PR ChinaDepartment of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering, Shanghai, 200011, PR China; National Tissue Engineering Center of China, Shanghai, 200241, PR ChinaNational Tissue Engineering Center of China, Shanghai, 200241, PR China; Corresponding author. National Tissue Engineering Center of China, Shanghai, 200241, PR China.Research Institute of Plastic Surgery, Wei Fang Medical University, Wei Fang, Shandong, 261041, PR China; Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering, Shanghai, 200011, PR China; National Tissue Engineering Center of China, Shanghai, 200241, PR China; Corresponding author. Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering, Shanghai, 200011, PR China.Research Institute of Plastic Surgery, Wei Fang Medical University, Wei Fang, Shandong, 261041, PR China; Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering, Shanghai, 200011, PR China; National Tissue Engineering Center of China, Shanghai, 200241, PR China; Corresponding author. Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering, Shanghai, 200011, PR China.Tissue engineering provides a new approach for the treatment of osteochondral defects. However, the lack of an ideal double-layer scaffold with osteochondral-biomimetic microenvironment and interface similar to native articular tissue greatly limits clinical translation. Our current study developed a double-layer acellular osteochondral matrix (AOM) scaffold with natural osteochondral-biomimetic microenvironment and interface by integrating ultraviolet (UV) laser and decellularization techniques. The laser parameters were optimized to achieve a proper pore depth close to the osteochondral interface, which guaranteed complete decellularization, sufficient space for cell loading, and relative independence of the chondrogenic and osteogenic microenvironments. Gelatin-methacryloyl (GelMA) hydrogel was further used as the cell carrier to significantly enhance the efficiency and homogeneity of cell loading in the AOM scaffold with large pore structure. Additionally, in vitro results demonstrated that the components of the AOM scaffold could efficiently regulate the chondrogenic/osteogenic differentiations of bone marrow stromal cells (BMSCs) by activating the chondrogenic/osteogenic related pathways. Importantly, the AOM scaffolds combined with BMSC-laden GelMA hydrogel successfully realized tissue-specific repair of the osteochondral defects in a knee joint model of rabbit. The current study developed a novel double-layer osteochondral biomimetic scaffold and feasible strategy, providing strong support for the tissue-specific repair of osteochondral defects and its future clinical translation.http://www.sciencedirect.com/science/article/pii/S2590006422000321Laser technologyAcellular osteochondral matrixBiomimetic scaffoldHydrogelOsteochondral defect |
| spellingShingle | Tao Wang Wei Xu Xintong Zhao Baoshuai Bai Yuejie Hua Jincheng Tang Feifan Chen Yu Liu Yahui Wang Guangdong Zhou Yilin Cao Repair of osteochondral defects mediated by double-layer scaffolds with natural osteochondral-biomimetic microenvironment and interface Materials Today Bio Laser technology Acellular osteochondral matrix Biomimetic scaffold Hydrogel Osteochondral defect |
| title | Repair of osteochondral defects mediated by double-layer scaffolds with natural osteochondral-biomimetic microenvironment and interface |
| title_full | Repair of osteochondral defects mediated by double-layer scaffolds with natural osteochondral-biomimetic microenvironment and interface |
| title_fullStr | Repair of osteochondral defects mediated by double-layer scaffolds with natural osteochondral-biomimetic microenvironment and interface |
| title_full_unstemmed | Repair of osteochondral defects mediated by double-layer scaffolds with natural osteochondral-biomimetic microenvironment and interface |
| title_short | Repair of osteochondral defects mediated by double-layer scaffolds with natural osteochondral-biomimetic microenvironment and interface |
| title_sort | repair of osteochondral defects mediated by double layer scaffolds with natural osteochondral biomimetic microenvironment and interface |
| topic | Laser technology Acellular osteochondral matrix Biomimetic scaffold Hydrogel Osteochondral defect |
| url | http://www.sciencedirect.com/science/article/pii/S2590006422000321 |
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