The GDSL-Lipolytic Enzyme Lip1 Is Required for Full Virulence of the Cucurbit Pathogenic Bacterium <i>Acidovorax citrulli</i>
Bacterial fruit blotch caused by <i>Acidovorax</i><i>citrulli</i> is a serious disease of cucurbit crops. Here we report characterization of a mutant strain of <i>A. citrulli</i> M6 defective in <i>lip1</i>, a gene encoding a lipolytic enzyme. The M6-&...
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MDPI AG
2022-05-01
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| Online Access: | https://www.mdpi.com/2076-2607/10/5/1016 |
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| author | Tally Rosenberg Irene Jiménez-Guerrero Dafna Tamir-Ariel Tali Yarnitzky Saul Burdman |
| author_facet | Tally Rosenberg Irene Jiménez-Guerrero Dafna Tamir-Ariel Tali Yarnitzky Saul Burdman |
| author_sort | Tally Rosenberg |
| collection | DOAJ |
| description | Bacterial fruit blotch caused by <i>Acidovorax</i><i>citrulli</i> is a serious disease of cucurbit crops. Here we report characterization of a mutant strain of <i>A. citrulli</i> M6 defective in <i>lip1</i>, a gene encoding a lipolytic enzyme. The M6-<i>lip1<sup>-</sup></i> mutant was detected in a mutant library screen aimed at identifying M6 mutants with altered levels of twitching motility. In this screen M6-<i>lip1<sup>-</sup></i> was the only mutant that showed significantly larger twitching motility haloes around colonies than wild-type M6. Sequence analyses indicated that <i>lip1</i> encodes a member of the GDSL family of secreted lipolytic enzymes. In line with this finding, lipolytic assays showed that the supernatants of M6-<i>lip1<sup>-</sup></i> had lower lipolytic activity as compared with those of wild-type M6 and a <i>lip1</i>-complemented strain. The mutant was also affected in swimming motility and had compromised virulence on melon seedlings and on <i>Nicotiana benthamiana</i> leaves relative to wild-type and complemented strains. Lip1 contains a predicted N-terminal signal sequence for type II secretion. Evidence from our study confirms Lip1 is indeed secreted in a type II secretion-dependent manner, and this is required for full virulence of <i>A. citrulli</i>. To the best of our knowledge this is the first study reporting contribution of lipolytic activity to virulence of a plant-pathogenic <i>Acidovorax</i> species. |
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| last_indexed | 2024-03-10T03:22:07Z |
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| spelling | doaj.art-7847adbb837d47ecbe15d3cb4a9097be2023-11-23T12:16:32ZengMDPI AGMicroorganisms2076-26072022-05-01105101610.3390/microorganisms10051016The GDSL-Lipolytic Enzyme Lip1 Is Required for Full Virulence of the Cucurbit Pathogenic Bacterium <i>Acidovorax citrulli</i>Tally Rosenberg0Irene Jiménez-Guerrero1Dafna Tamir-Ariel2Tali Yarnitzky3Saul Burdman4Department of Plant Pathology and Microbiology, Institute of Environmental Sciences, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 7610001, IsraelDepartment of Plant Pathology and Microbiology, Institute of Environmental Sciences, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 7610001, IsraelDepartment of Plant Pathology and Microbiology, Institute of Environmental Sciences, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 7610001, IsraelDepartment of Plant Pathology and Microbiology, Institute of Environmental Sciences, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 7610001, IsraelDepartment of Plant Pathology and Microbiology, Institute of Environmental Sciences, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 7610001, IsraelBacterial fruit blotch caused by <i>Acidovorax</i><i>citrulli</i> is a serious disease of cucurbit crops. Here we report characterization of a mutant strain of <i>A. citrulli</i> M6 defective in <i>lip1</i>, a gene encoding a lipolytic enzyme. The M6-<i>lip1<sup>-</sup></i> mutant was detected in a mutant library screen aimed at identifying M6 mutants with altered levels of twitching motility. In this screen M6-<i>lip1<sup>-</sup></i> was the only mutant that showed significantly larger twitching motility haloes around colonies than wild-type M6. Sequence analyses indicated that <i>lip1</i> encodes a member of the GDSL family of secreted lipolytic enzymes. In line with this finding, lipolytic assays showed that the supernatants of M6-<i>lip1<sup>-</sup></i> had lower lipolytic activity as compared with those of wild-type M6 and a <i>lip1</i>-complemented strain. The mutant was also affected in swimming motility and had compromised virulence on melon seedlings and on <i>Nicotiana benthamiana</i> leaves relative to wild-type and complemented strains. Lip1 contains a predicted N-terminal signal sequence for type II secretion. Evidence from our study confirms Lip1 is indeed secreted in a type II secretion-dependent manner, and this is required for full virulence of <i>A. citrulli</i>. To the best of our knowledge this is the first study reporting contribution of lipolytic activity to virulence of a plant-pathogenic <i>Acidovorax</i> species.https://www.mdpi.com/2076-2607/10/5/1016<i>Acidovorax citrulli</i>bacterial fruit blotchlipaseesterasevirulencetype IV pili |
| spellingShingle | Tally Rosenberg Irene Jiménez-Guerrero Dafna Tamir-Ariel Tali Yarnitzky Saul Burdman The GDSL-Lipolytic Enzyme Lip1 Is Required for Full Virulence of the Cucurbit Pathogenic Bacterium <i>Acidovorax citrulli</i> Microorganisms <i>Acidovorax citrulli</i> bacterial fruit blotch lipase esterase virulence type IV pili |
| title | The GDSL-Lipolytic Enzyme Lip1 Is Required for Full Virulence of the Cucurbit Pathogenic Bacterium <i>Acidovorax citrulli</i> |
| title_full | The GDSL-Lipolytic Enzyme Lip1 Is Required for Full Virulence of the Cucurbit Pathogenic Bacterium <i>Acidovorax citrulli</i> |
| title_fullStr | The GDSL-Lipolytic Enzyme Lip1 Is Required for Full Virulence of the Cucurbit Pathogenic Bacterium <i>Acidovorax citrulli</i> |
| title_full_unstemmed | The GDSL-Lipolytic Enzyme Lip1 Is Required for Full Virulence of the Cucurbit Pathogenic Bacterium <i>Acidovorax citrulli</i> |
| title_short | The GDSL-Lipolytic Enzyme Lip1 Is Required for Full Virulence of the Cucurbit Pathogenic Bacterium <i>Acidovorax citrulli</i> |
| title_sort | gdsl lipolytic enzyme lip1 is required for full virulence of the cucurbit pathogenic bacterium i acidovorax citrulli i |
| topic | <i>Acidovorax citrulli</i> bacterial fruit blotch lipase esterase virulence type IV pili |
| url | https://www.mdpi.com/2076-2607/10/5/1016 |
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