The rkp-3 Gene Region of Sinorhizobium meliloti Rm41 Contains Strain-Specific Genes that Determine K Antigen Structure
The rkp-3 region is indispensable for capsular polysaccharide (K antigen) synthesis in Sinorhizobium meliloti Rm41. Strain Rm41 produces a K antigen of strain-specific structure, designated as the KR5 antigen. The data in this report show that the rkp-3 gene region comprises 10 open reading frames i...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2001-12-01
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| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2001.14.12.1395 |
| _version_ | 1818647230332010496 |
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| author | Ernő Kiss Attila Kereszt Fatime Barta Samuel Stephens Bradley L. Reuhs Ádám Kondorosi Péter Putnoky |
| author_facet | Ernő Kiss Attila Kereszt Fatime Barta Samuel Stephens Bradley L. Reuhs Ádám Kondorosi Péter Putnoky |
| author_sort | Ernő Kiss |
| collection | DOAJ |
| description | The rkp-3 region is indispensable for capsular polysaccharide (K antigen) synthesis in Sinorhizobium meliloti Rm41. Strain Rm41 produces a K antigen of strain-specific structure, designated as the KR5 antigen. The data in this report show that the rkp-3 gene region comprises 10 open reading frames involved in bacterial polysaccharide synthesis and export. The predicted amino acid sequences for the rkpL-Q gene products are homologous to enzymes involved in the production of specific sugar moieties, while the putative products of the rkpRST genes show a high degree of similarity to proteins required for transporting polysaccharides to the cell surface. Southern analysis experiments using gene-specific probes suggest that genes involved in the synthesis of the precursor sugars are unique in strain Rm41, whereas sequences coding for export proteins are widely distributed among Sinorhizobium species. Mutations in the rkpL-Q genes result in a modified K antigen pattern and impaired symbiotic capabilities. On this basis, we suggest that these genes are required for the production of the KR5 antigen that is necessary for S. meliloti Rm41 exoB (AK631)-alfalfa (Medicago sativa) symbiosis. |
| first_indexed | 2024-12-17T00:59:14Z |
| format | Article |
| id | doaj.art-784a71a1130249808756374ffc68e07d |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-12-17T00:59:14Z |
| publishDate | 2001-12-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-784a71a1130249808756374ffc68e07d2022-12-21T22:09:31ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062001-12-0114121395140310.1094/MPMI.2001.14.12.1395The rkp-3 Gene Region of Sinorhizobium meliloti Rm41 Contains Strain-Specific Genes that Determine K Antigen StructureErnő KissAttila KeresztFatime BartaSamuel StephensBradley L. ReuhsÁdám KondorosiPéter PutnokyThe rkp-3 region is indispensable for capsular polysaccharide (K antigen) synthesis in Sinorhizobium meliloti Rm41. Strain Rm41 produces a K antigen of strain-specific structure, designated as the KR5 antigen. The data in this report show that the rkp-3 gene region comprises 10 open reading frames involved in bacterial polysaccharide synthesis and export. The predicted amino acid sequences for the rkpL-Q gene products are homologous to enzymes involved in the production of specific sugar moieties, while the putative products of the rkpRST genes show a high degree of similarity to proteins required for transporting polysaccharides to the cell surface. Southern analysis experiments using gene-specific probes suggest that genes involved in the synthesis of the precursor sugars are unique in strain Rm41, whereas sequences coding for export proteins are widely distributed among Sinorhizobium species. Mutations in the rkpL-Q genes result in a modified K antigen pattern and impaired symbiotic capabilities. On this basis, we suggest that these genes are required for the production of the KR5 antigen that is necessary for S. meliloti Rm41 exoB (AK631)-alfalfa (Medicago sativa) symbiosis.https://apsjournals.apsnet.org/doi/10.1094/MPMI.2001.14.12.1395pseudaminic acid |
| spellingShingle | Ernő Kiss Attila Kereszt Fatime Barta Samuel Stephens Bradley L. Reuhs Ádám Kondorosi Péter Putnoky The rkp-3 Gene Region of Sinorhizobium meliloti Rm41 Contains Strain-Specific Genes that Determine K Antigen Structure Molecular Plant-Microbe Interactions pseudaminic acid |
| title | The rkp-3 Gene Region of Sinorhizobium meliloti Rm41 Contains Strain-Specific Genes that Determine K Antigen Structure |
| title_full | The rkp-3 Gene Region of Sinorhizobium meliloti Rm41 Contains Strain-Specific Genes that Determine K Antigen Structure |
| title_fullStr | The rkp-3 Gene Region of Sinorhizobium meliloti Rm41 Contains Strain-Specific Genes that Determine K Antigen Structure |
| title_full_unstemmed | The rkp-3 Gene Region of Sinorhizobium meliloti Rm41 Contains Strain-Specific Genes that Determine K Antigen Structure |
| title_short | The rkp-3 Gene Region of Sinorhizobium meliloti Rm41 Contains Strain-Specific Genes that Determine K Antigen Structure |
| title_sort | rkp 3 gene region of sinorhizobium meliloti rm41 contains strain specific genes that determine k antigen structure |
| topic | pseudaminic acid |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2001.14.12.1395 |
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