Determination of 4 Kinds of β-Agonists Residues in Braised Meat by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry
An ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS) method was developed for the determination of four β-agonists (terbutaline, clenbuterol, ractopamine, salbutamol) in braised meat. Samples were hydrolyzed by β-glucuronidase and cleaned up by an SLS solid phase extrac...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | zho |
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The editorial department of Science and Technology of Food Industry
2024-01-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2023030044 |
| _version_ | 1797376641035403264 |
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| author | Chunyang MENG Yutian WU Lei PENG Xue ZHONG Lu ZOU Wenzheng LIU Yibing ZHOU |
| author_facet | Chunyang MENG Yutian WU Lei PENG Xue ZHONG Lu ZOU Wenzheng LIU Yibing ZHOU |
| author_sort | Chunyang MENG |
| collection | DOAJ |
| description | An ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS) method was developed for the determination of four β-agonists (terbutaline, clenbuterol, ractopamine, salbutamol) in braised meat. Samples were hydrolyzed by β-glucuronidase and cleaned up by an SLS solid phase extraction column. The separation was performed on a Thermo Hypersil Gold C18 column with a gradient elution of 0.1% formic acid water and acetonitrile as mobile phases, ESI+ was used for multiple response monitoring (MRM) and quantitative analysis by internal standard method. The linear relationship of the four β-agonists was good in the concentration range of 0.5 μg/L to 9.5 μg/L, and the correlation coefficient (r) was greater than 0.9988. The limit of detection (LOD) was 0.1 μg/kg, and the limit of quantitation (LOQ) was 0.3 μg/kg. The recoveries were 87.9%~113.7% and RSDs were 1.48%~9.32% at three spiked levels (1, 5 and 9 μg/kg). In a total of 162 batches of braised meat samples, one sample of braised pig’s trotter was found to contain 1.51 μg/kg of clenbuterol and 3.65 μg/kg of ractopamine. Additionally, another sample of braised lamb was found to contain 11.5 μg/kg of clenbuterol. The method is rapid and accurate, and can be used for qualitative and quantitative determination of four β-agonists (terbutaline, clenbuterol, ractopamine, salbutamol) in braised meat. |
| first_indexed | 2024-03-08T19:41:34Z |
| format | Article |
| id | doaj.art-784b1a7b12304bee89cfdf4d0eda1bd8 |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-03-08T19:41:34Z |
| publishDate | 2024-01-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-784b1a7b12304bee89cfdf4d0eda1bd82023-12-25T05:30:37ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062024-01-0145127728310.13386/j.issn1002-0306.20230300442023030044-1Determination of 4 Kinds of β-Agonists Residues in Braised Meat by Ultra Performance Liquid Chromatography-Tandem Mass SpectrometryChunyang MENG0Yutian WU1Lei PENG2Xue ZHONG3Lu ZOU4Wenzheng LIU5Yibing ZHOU6Guizhou Provincial Center for Disease Control and Prevention, Guiyang 550004, ChinaGuizhou Provincial Center for Disease Control and Prevention, Guiyang 550004, ChinaGuizhou Provincial Center for Disease Control and Prevention, Guiyang 550004, ChinaGuizhou Provincial Center for Disease Control and Prevention, Guiyang 550004, ChinaGuizhou Provincial Center for Disease Control and Prevention, Guiyang 550004, ChinaGuizhou Provincial Center for Disease Control and Prevention, Guiyang 550004, ChinaGuizhou Provincial Center for Disease Control and Prevention, Guiyang 550004, ChinaAn ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS) method was developed for the determination of four β-agonists (terbutaline, clenbuterol, ractopamine, salbutamol) in braised meat. Samples were hydrolyzed by β-glucuronidase and cleaned up by an SLS solid phase extraction column. The separation was performed on a Thermo Hypersil Gold C18 column with a gradient elution of 0.1% formic acid water and acetonitrile as mobile phases, ESI+ was used for multiple response monitoring (MRM) and quantitative analysis by internal standard method. The linear relationship of the four β-agonists was good in the concentration range of 0.5 μg/L to 9.5 μg/L, and the correlation coefficient (r) was greater than 0.9988. The limit of detection (LOD) was 0.1 μg/kg, and the limit of quantitation (LOQ) was 0.3 μg/kg. The recoveries were 87.9%~113.7% and RSDs were 1.48%~9.32% at three spiked levels (1, 5 and 9 μg/kg). In a total of 162 batches of braised meat samples, one sample of braised pig’s trotter was found to contain 1.51 μg/kg of clenbuterol and 3.65 μg/kg of ractopamine. Additionally, another sample of braised lamb was found to contain 11.5 μg/kg of clenbuterol. The method is rapid and accurate, and can be used for qualitative and quantitative determination of four β-agonists (terbutaline, clenbuterol, ractopamine, salbutamol) in braised meat.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2023030044ultra performance liquid chromatography-tandem mass spectrometry (uplc-ms)braised meatβ-agonistssls solid phase extraction columnisotope internal standard |
| spellingShingle | Chunyang MENG Yutian WU Lei PENG Xue ZHONG Lu ZOU Wenzheng LIU Yibing ZHOU Determination of 4 Kinds of β-Agonists Residues in Braised Meat by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry Shipin gongye ke-ji ultra performance liquid chromatography-tandem mass spectrometry (uplc-ms) braised meat β-agonists sls solid phase extraction column isotope internal standard |
| title | Determination of 4 Kinds of β-Agonists Residues in Braised Meat by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry |
| title_full | Determination of 4 Kinds of β-Agonists Residues in Braised Meat by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry |
| title_fullStr | Determination of 4 Kinds of β-Agonists Residues in Braised Meat by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry |
| title_full_unstemmed | Determination of 4 Kinds of β-Agonists Residues in Braised Meat by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry |
| title_short | Determination of 4 Kinds of β-Agonists Residues in Braised Meat by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry |
| title_sort | determination of 4 kinds of β agonists residues in braised meat by ultra performance liquid chromatography tandem mass spectrometry |
| topic | ultra performance liquid chromatography-tandem mass spectrometry (uplc-ms) braised meat β-agonists sls solid phase extraction column isotope internal standard |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2023030044 |
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