Lower peripheral blood Toll-like receptor 3 expression is associated with an unfavorable outcome in severe COVID-19 patients
Abstract The role of innate immunity in COVID-19 is not completely understood. Therefore, this study explored the impact of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection on the expression of Pattern Recognition Receptors (PRRs) in peripheral blood cells and their correlated...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-94624-4 |
| _version_ | 1818841257297838080 |
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| author | Maria Clara Saad Menezes Alicia Dudy Müller Veiga Thais Martins de Lima Suely Kunimi Kubo Ariga Hermes Vieira Barbeiro Claudia de Lucena Moreira Agnes Araujo Sardinha Pinto Rodrigo Antonio Brandao Julio Flavio Marchini Julio Cesar Alencar Lucas Oliveira Marino Luz Marina Gomez Niels Olsen Saraiva Camara Heraldo P. Souza |
| author_facet | Maria Clara Saad Menezes Alicia Dudy Müller Veiga Thais Martins de Lima Suely Kunimi Kubo Ariga Hermes Vieira Barbeiro Claudia de Lucena Moreira Agnes Araujo Sardinha Pinto Rodrigo Antonio Brandao Julio Flavio Marchini Julio Cesar Alencar Lucas Oliveira Marino Luz Marina Gomez Niels Olsen Saraiva Camara Heraldo P. Souza |
| author_sort | Maria Clara Saad Menezes |
| collection | DOAJ |
| description | Abstract The role of innate immunity in COVID-19 is not completely understood. Therefore, this study explored the impact of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection on the expression of Pattern Recognition Receptors (PRRs) in peripheral blood cells and their correlated cytokines. Seventy-nine patients with severe COVID-19 on admission, according to World Health Organization (WHO) classification, were divided into two groups: patients who needed mechanical ventilation and/or deceased (SEVERE, n = 50) and patients who used supplementary oxygen but not mechanical ventilation and survived (MILD, n = 29); a control group (CONTROL, n = 17) was also enrolled. In the peripheral blood, gene expression (mRNA) of Toll-like receptors (TLRs) 3, 4, 7, 8, and 9, retinoic-acid inducible gene I (RIGI), NOD-like receptor family pyrin domain containing 3 (NLRP3), interferon alpha (IFN-α), interferon beta (IFN-β), interferon gamma (IFN-γ), interferon lambda (IFN-λ), pro-interleukin(IL)-1β (pro-IL-1β), and IL-18 was determined on admission, between 5–9 days, and between 10–15 days. Circulating cytokines in plasma were also measured. When compared to the COVID-19 MILD group, the COVID-19 SEVERE group had lower expression of TLR3 and overexpression of TLR4. |
| first_indexed | 2024-12-19T04:23:12Z |
| format | Article |
| id | doaj.art-784c55093fb949b8b9d65c54ee160321 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-19T04:23:12Z |
| publishDate | 2021-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-784c55093fb949b8b9d65c54ee1603212022-12-21T20:36:06ZengNature PortfolioScientific Reports2045-23222021-07-0111111210.1038/s41598-021-94624-4Lower peripheral blood Toll-like receptor 3 expression is associated with an unfavorable outcome in severe COVID-19 patientsMaria Clara Saad Menezes0Alicia Dudy Müller Veiga1Thais Martins de Lima2Suely Kunimi Kubo Ariga3Hermes Vieira Barbeiro4Claudia de Lucena Moreira5Agnes Araujo Sardinha Pinto6Rodrigo Antonio Brandao7Julio Flavio Marchini8Julio Cesar Alencar9Lucas Oliveira Marino10Luz Marina Gomez11Niels Olsen Saraiva Camara12Heraldo P. Souza13Emergency Medicine Department, Faculdade de Medicina da Universidade de São PauloEmergency Medicine Department, Faculdade de Medicina da Universidade de São PauloEmergency Medicine Department, Faculdade de Medicina da Universidade de São PauloEmergency Medicine Department, Faculdade de Medicina da Universidade de São PauloEmergency Medicine Department, Faculdade de Medicina da Universidade de São PauloEmergency Medicine Department, Faculdade de Medicina da Universidade de São PauloEmergency Medicine Department, Faculdade de Medicina da Universidade de São PauloEmergency Medicine Department, Faculdade de Medicina da Universidade de São PauloEmergency Medicine Department, Faculdade de Medicina da Universidade de São PauloEmergency Medicine Department, Faculdade de Medicina da Universidade de São PauloEmergency Medicine Department, Faculdade de Medicina da Universidade de São PauloEmergency Medicine Department, Faculdade de Medicina da Universidade de São PauloDepartment of Immunology, Institute of Biomedical Sciences, Universidade de São PauloEmergency Medicine Department, Faculdade de Medicina da Universidade de São PauloAbstract The role of innate immunity in COVID-19 is not completely understood. Therefore, this study explored the impact of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection on the expression of Pattern Recognition Receptors (PRRs) in peripheral blood cells and their correlated cytokines. Seventy-nine patients with severe COVID-19 on admission, according to World Health Organization (WHO) classification, were divided into two groups: patients who needed mechanical ventilation and/or deceased (SEVERE, n = 50) and patients who used supplementary oxygen but not mechanical ventilation and survived (MILD, n = 29); a control group (CONTROL, n = 17) was also enrolled. In the peripheral blood, gene expression (mRNA) of Toll-like receptors (TLRs) 3, 4, 7, 8, and 9, retinoic-acid inducible gene I (RIGI), NOD-like receptor family pyrin domain containing 3 (NLRP3), interferon alpha (IFN-α), interferon beta (IFN-β), interferon gamma (IFN-γ), interferon lambda (IFN-λ), pro-interleukin(IL)-1β (pro-IL-1β), and IL-18 was determined on admission, between 5–9 days, and between 10–15 days. Circulating cytokines in plasma were also measured. When compared to the COVID-19 MILD group, the COVID-19 SEVERE group had lower expression of TLR3 and overexpression of TLR4.https://doi.org/10.1038/s41598-021-94624-4 |
| spellingShingle | Maria Clara Saad Menezes Alicia Dudy Müller Veiga Thais Martins de Lima Suely Kunimi Kubo Ariga Hermes Vieira Barbeiro Claudia de Lucena Moreira Agnes Araujo Sardinha Pinto Rodrigo Antonio Brandao Julio Flavio Marchini Julio Cesar Alencar Lucas Oliveira Marino Luz Marina Gomez Niels Olsen Saraiva Camara Heraldo P. Souza Lower peripheral blood Toll-like receptor 3 expression is associated with an unfavorable outcome in severe COVID-19 patients Scientific Reports |
| title | Lower peripheral blood Toll-like receptor 3 expression is associated with an unfavorable outcome in severe COVID-19 patients |
| title_full | Lower peripheral blood Toll-like receptor 3 expression is associated with an unfavorable outcome in severe COVID-19 patients |
| title_fullStr | Lower peripheral blood Toll-like receptor 3 expression is associated with an unfavorable outcome in severe COVID-19 patients |
| title_full_unstemmed | Lower peripheral blood Toll-like receptor 3 expression is associated with an unfavorable outcome in severe COVID-19 patients |
| title_short | Lower peripheral blood Toll-like receptor 3 expression is associated with an unfavorable outcome in severe COVID-19 patients |
| title_sort | lower peripheral blood toll like receptor 3 expression is associated with an unfavorable outcome in severe covid 19 patients |
| url | https://doi.org/10.1038/s41598-021-94624-4 |
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