Biomarker potential of hsa-miR-145-5p in peripheral whole blood of manic bipolar I patients
Objective: Bipolar I disorder (BD-I) is a type of bipolar spectrum disorder characterized by manic or mixed episodes. Detecting microRNA regulations as epigenetic actors in BD-I is important to elucidate the pathogenesis of the disease and reveal the potential of microRNAs (miRNAs) as biomarkers....
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Psiquiatria (ABP)
2022-06-01
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| Series: | Brazilian Journal of Psychiatry |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-44462022005006206&tlng=en |
| _version_ | 1811344431138209792 |
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| author | Sevinç Sürer Tekin Mehmet Emin Erdal Mehmet Asoğlu Özlem İzci Ay Mustafa Ertan Ay Şenay Görücü Yılmaz |
| author_facet | Sevinç Sürer Tekin Mehmet Emin Erdal Mehmet Asoğlu Özlem İzci Ay Mustafa Ertan Ay Şenay Görücü Yılmaz |
| author_sort | Sevinç Sürer Tekin |
| collection | DOAJ |
| description | Objective: Bipolar I disorder (BD-I) is a type of bipolar spectrum disorder characterized by manic or mixed episodes. Detecting microRNA regulations as epigenetic actors in BD-I is important to elucidate the pathogenesis of the disease and reveal the potential of microRNAs (miRNAs) as biomarkers. Methods: We evaluated the expression profile of six candidate miRNAs (hsa-miR-145-5p, hsa-miR-376a-3p, hsa-miR-3680-5p, hsa-miR-4253-5p, hsa-miR-4482-3p, and hsa-miR-4725) in patients with BD-I and in healthy controls (aged 11-50 years). We also determined the potential target genes of these miRNAs through in silico analysis. The diagnostic values of the miRNAs were calculated through receiver operating characteristic curve analysis. Results: Four miRNAs were upregulated (hsa-miR-376a-3p, hsa-miR-3680-5p, hsa-miR-4253-5p, hsa-miR-4482-3p) and hsa-miR-145-5p was downregulated in patients (p < 0.001). The target gene analyses showed that hsa-miR-145-5p specifically targets the dopamine decarboxylase (DDC) gene. The area under the curve of hsa-miR-145-5p was 0.987. Conclusion: Differential expression of five miRNAs in peripheral blood may be associated with the pathogenesis of BD-I, and hsa-miR-145-5p has potential as a BD-I biomarker. This miRNA can be used in dopamine-serotonin regulation and dose adjustment in drug therapy via the DDC gene. |
| first_indexed | 2024-04-13T19:47:13Z |
| format | Article |
| id | doaj.art-784c6f5b212c4f05a4e66573fe169401 |
| institution | Directory Open Access Journal |
| issn | 1809-452X |
| language | English |
| last_indexed | 2024-04-13T19:47:13Z |
| publishDate | 2022-06-01 |
| publisher | Associação Brasileira de Psiquiatria (ABP) |
| record_format | Article |
| series | Brazilian Journal of Psychiatry |
| spelling | doaj.art-784c6f5b212c4f05a4e66573fe1694012022-12-22T02:32:41ZengAssociação Brasileira de Psiquiatria (ABP)Brazilian Journal of Psychiatry1809-452X2022-06-0110.47626/1516-4446-2021-2260Biomarker potential of hsa-miR-145-5p in peripheral whole blood of manic bipolar I patientsSevinç Sürer Tekinhttps://orcid.org/0000-0002-8502-0400Mehmet Emin Erdalhttps://orcid.org/0000-0002-6191-2930Mehmet Asoğluhttps://orcid.org/0000-0001-7515-7844Özlem İzci Ayhttps://orcid.org/0000-0002-4847-6943Mustafa Ertan Ayhttps://orcid.org/0000-0002-6152-7450Şenay Görücü Yılmazhttps://orcid.org/0000-0003-0523-7819 Objective: Bipolar I disorder (BD-I) is a type of bipolar spectrum disorder characterized by manic or mixed episodes. Detecting microRNA regulations as epigenetic actors in BD-I is important to elucidate the pathogenesis of the disease and reveal the potential of microRNAs (miRNAs) as biomarkers. Methods: We evaluated the expression profile of six candidate miRNAs (hsa-miR-145-5p, hsa-miR-376a-3p, hsa-miR-3680-5p, hsa-miR-4253-5p, hsa-miR-4482-3p, and hsa-miR-4725) in patients with BD-I and in healthy controls (aged 11-50 years). We also determined the potential target genes of these miRNAs through in silico analysis. The diagnostic values of the miRNAs were calculated through receiver operating characteristic curve analysis. Results: Four miRNAs were upregulated (hsa-miR-376a-3p, hsa-miR-3680-5p, hsa-miR-4253-5p, hsa-miR-4482-3p) and hsa-miR-145-5p was downregulated in patients (p < 0.001). The target gene analyses showed that hsa-miR-145-5p specifically targets the dopamine decarboxylase (DDC) gene. The area under the curve of hsa-miR-145-5p was 0.987. Conclusion: Differential expression of five miRNAs in peripheral blood may be associated with the pathogenesis of BD-I, and hsa-miR-145-5p has potential as a BD-I biomarker. This miRNA can be used in dopamine-serotonin regulation and dose adjustment in drug therapy via the DDC gene.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-44462022005006206&tlng=enManicbipolar I disorderbiomarkermiR-145 |
| spellingShingle | Sevinç Sürer Tekin Mehmet Emin Erdal Mehmet Asoğlu Özlem İzci Ay Mustafa Ertan Ay Şenay Görücü Yılmaz Biomarker potential of hsa-miR-145-5p in peripheral whole blood of manic bipolar I patients Brazilian Journal of Psychiatry Manic bipolar I disorder biomarker miR-145 |
| title | Biomarker potential of hsa-miR-145-5p in peripheral whole blood of manic bipolar I patients |
| title_full | Biomarker potential of hsa-miR-145-5p in peripheral whole blood of manic bipolar I patients |
| title_fullStr | Biomarker potential of hsa-miR-145-5p in peripheral whole blood of manic bipolar I patients |
| title_full_unstemmed | Biomarker potential of hsa-miR-145-5p in peripheral whole blood of manic bipolar I patients |
| title_short | Biomarker potential of hsa-miR-145-5p in peripheral whole blood of manic bipolar I patients |
| title_sort | biomarker potential of hsa mir 145 5p in peripheral whole blood of manic bipolar i patients |
| topic | Manic bipolar I disorder biomarker miR-145 |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-44462022005006206&tlng=en |
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