Sleeping ribosomes: Bacterial signaling triggers RaiA mediated persistence to aminoglycosides
Summary: Indole is a molecule proposed to be involved in bacterial signaling. We find that indole secretion is induced by sublethal tobramycin concentrations and increases persistence to aminoglycosides in V. cholerae. Indole transcriptomics showed increased expression of raiA, a ribosome associated...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-10-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004221010968 |
| _version_ | 1819030803467730944 |
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| author | Manon Lang Evelyne Krin Chloé Korlowski Odile Sismeiro Hugo Varet Jean-Yves Coppée Didier Mazel Zeynep Baharoglu |
| author_facet | Manon Lang Evelyne Krin Chloé Korlowski Odile Sismeiro Hugo Varet Jean-Yves Coppée Didier Mazel Zeynep Baharoglu |
| author_sort | Manon Lang |
| collection | DOAJ |
| description | Summary: Indole is a molecule proposed to be involved in bacterial signaling. We find that indole secretion is induced by sublethal tobramycin concentrations and increases persistence to aminoglycosides in V. cholerae. Indole transcriptomics showed increased expression of raiA, a ribosome associated factor. Deletion of raiA abolishes the appearance of indole dependent persisters to aminoglycosides, although its overexpression leads to 100-fold increase of persisters, and a reduction in lag phase, evocative of increased active 70S ribosome content, confirmed by sucrose gradient analysis. We propose that, under stress conditions, RaiA-bound inactive 70S ribosomes are stored as “sleeping ribosomes”, and are rapidly reactivated upon stress relief. Our results point to an active process of persister formation through ribosome protection during translational stress (e.g., aminoglycoside treatment) and reactivation upon antibiotic removal. Translation is a universal process, and these results could help elucidate a mechanism of persistence formation in a controlled, thus inducible way. |
| first_indexed | 2024-12-21T06:35:58Z |
| format | Article |
| id | doaj.art-784da69c8c9d4db98a7208901f9df388 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-12-21T06:35:58Z |
| publishDate | 2021-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-784da69c8c9d4db98a7208901f9df3882022-12-21T19:12:52ZengElsevieriScience2589-00422021-10-012410103128Sleeping ribosomes: Bacterial signaling triggers RaiA mediated persistence to aminoglycosidesManon Lang0Evelyne Krin1Chloé Korlowski2Odile Sismeiro3Hugo Varet4Jean-Yves Coppée5Didier Mazel6Zeynep Baharoglu7Département Génomes et Génétique, Institut Pasteur, UMR3525, CNRS, Unité Plasticité du Génome Bactérien, 75015 Paris, France; Sorbonne Université, Collège Doctoral, 75005 Paris, FranceDépartement Génomes et Génétique, Institut Pasteur, UMR3525, CNRS, Unité Plasticité du Génome Bactérien, 75015 Paris, FranceDépartement Génomes et Génétique, Institut Pasteur, UMR3525, CNRS, Unité Plasticité du Génome Bactérien, 75015 Paris, FranceBiomics Technological Platform, Center for Technological Resources and Research, Institut Pasteur, 75015 Paris, FranceBiomics Technological Platform, Center for Technological Resources and Research, Institut Pasteur, 75015 Paris, France; Bioinformatics and Biostatistics Hub, Department of Computational Biology, USR 3756 CNRS, Institut Pasteur, 75015 Paris, FranceBiomics Technological Platform, Center for Technological Resources and Research, Institut Pasteur, 75015 Paris, FranceDépartement Génomes et Génétique, Institut Pasteur, UMR3525, CNRS, Unité Plasticité du Génome Bactérien, 75015 Paris, France; Corresponding authorDépartement Génomes et Génétique, Institut Pasteur, UMR3525, CNRS, Unité Plasticité du Génome Bactérien, 75015 Paris, France; Corresponding authorSummary: Indole is a molecule proposed to be involved in bacterial signaling. We find that indole secretion is induced by sublethal tobramycin concentrations and increases persistence to aminoglycosides in V. cholerae. Indole transcriptomics showed increased expression of raiA, a ribosome associated factor. Deletion of raiA abolishes the appearance of indole dependent persisters to aminoglycosides, although its overexpression leads to 100-fold increase of persisters, and a reduction in lag phase, evocative of increased active 70S ribosome content, confirmed by sucrose gradient analysis. We propose that, under stress conditions, RaiA-bound inactive 70S ribosomes are stored as “sleeping ribosomes”, and are rapidly reactivated upon stress relief. Our results point to an active process of persister formation through ribosome protection during translational stress (e.g., aminoglycoside treatment) and reactivation upon antibiotic removal. Translation is a universal process, and these results could help elucidate a mechanism of persistence formation in a controlled, thus inducible way.http://www.sciencedirect.com/science/article/pii/S2589004221010968BacteriologyMicrobial genomicsViral microbiology |
| spellingShingle | Manon Lang Evelyne Krin Chloé Korlowski Odile Sismeiro Hugo Varet Jean-Yves Coppée Didier Mazel Zeynep Baharoglu Sleeping ribosomes: Bacterial signaling triggers RaiA mediated persistence to aminoglycosides iScience Bacteriology Microbial genomics Viral microbiology |
| title | Sleeping ribosomes: Bacterial signaling triggers RaiA mediated persistence to aminoglycosides |
| title_full | Sleeping ribosomes: Bacterial signaling triggers RaiA mediated persistence to aminoglycosides |
| title_fullStr | Sleeping ribosomes: Bacterial signaling triggers RaiA mediated persistence to aminoglycosides |
| title_full_unstemmed | Sleeping ribosomes: Bacterial signaling triggers RaiA mediated persistence to aminoglycosides |
| title_short | Sleeping ribosomes: Bacterial signaling triggers RaiA mediated persistence to aminoglycosides |
| title_sort | sleeping ribosomes bacterial signaling triggers raia mediated persistence to aminoglycosides |
| topic | Bacteriology Microbial genomics Viral microbiology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004221010968 |
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