Effect of exogenous microorganisms on the fermentation quality, nitrate degradation and bacterial community of sorghum-sudangrass silage
This study aims to investigate the effects of adding Lactobacillus buchneri (LB), Lactobacillus brevis (LBR) and Bacillus subtilis (BS) on the fermentation quality, nitrate degradation and bacterial community of sorghum-sudangrass silage. The results showed that the addition of LB significantly incr...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1052837/full |
| _version_ | 1798046466811363328 |
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| author | Meirong Zhao Hongyu Zhang Gang Pan Hang Yin Juanjuan Sun Zhu Yu Chunsheng Bai Yanlin Xue Yanlin Xue |
| author_facet | Meirong Zhao Hongyu Zhang Gang Pan Hang Yin Juanjuan Sun Zhu Yu Chunsheng Bai Yanlin Xue Yanlin Xue |
| author_sort | Meirong Zhao |
| collection | DOAJ |
| description | This study aims to investigate the effects of adding Lactobacillus buchneri (LB), Lactobacillus brevis (LBR) and Bacillus subtilis (BS) on the fermentation quality, nitrate degradation and bacterial community of sorghum-sudangrass silage. The results showed that the addition of LB significantly increased the pH and acetic acid content (p < 0.05), but high-quality silage was obtained. The addition of LBR and BS improved the fermentation quality of sorghum-sudangrass silage. The use of additives reduced the nitrate content in sorghum-sudangrass silage. The LB group increased the release of N2O at 3–7 days of ensiling (p < 0.05), and LBR and BS increased the release of N2O at 1–40 days of ensiling (p < 0.05). On the first day of ensiling, all silages were dominated by Weisslla, over 3 days of ensiling all silages were dominated by Lactobacillus. Acinetobacter, Serratia, Aquabacterium, and unclassified_f_enterobacteriaceae showed significant negative correlations with nitrate degradation during sorghum-sudangrass ensiling (p < 0.05). The BS and LBR groups increased the metabolic abundance of denitrification, dissimilatory nitrate reduction, and assimilatory nitrate reduction (p < 0.05). Overall, the additive ensures the fermentation quality of sorghum-sudangrass silage and promotes the degradation of nitrate by altering the bacterial community. |
| first_indexed | 2024-04-11T23:38:00Z |
| format | Article |
| id | doaj.art-784f11acfffe4845a3232766f24ef31e |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-11T23:38:00Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-784f11acfffe4845a3232766f24ef31e2022-12-22T03:56:53ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-11-011310.3389/fmicb.2022.10528371052837Effect of exogenous microorganisms on the fermentation quality, nitrate degradation and bacterial community of sorghum-sudangrass silageMeirong Zhao0Hongyu Zhang1Gang Pan2Hang Yin3Juanjuan Sun4Zhu Yu5Chunsheng Bai6Yanlin Xue7Yanlin Xue8College of Horticulture, Shenyang Agricultural University, Shenyang, ChinaCollege of Horticulture, Shenyang Agricultural University, Shenyang, ChinaCollege of Horticulture, Shenyang Agricultural University, Shenyang, ChinaCollege of Horticulture, Shenyang Agricultural University, Shenyang, ChinaInstitute of Grassland Research, Chinese Academy of Agricultural Sciences, Hohhot, ChinaCollege of Grassland Science and Technology, China Agricultural University, Beijing, ChinaCollege of Horticulture, Shenyang Agricultural University, Shenyang, ChinaInner Mongolia Engineering Research Center of Development and Utilization of Microbial Resources in Silage, Inner Mongolia Academy of Agriculture and Animal Husbandry Science, Hohhot, ChinaInner Mongolia Key Laboratory of Microbial Ecology of Silage, Inner Mongolia Academy of Agriculture and Animal Husbandry Science, Hohhot, ChinaThis study aims to investigate the effects of adding Lactobacillus buchneri (LB), Lactobacillus brevis (LBR) and Bacillus subtilis (BS) on the fermentation quality, nitrate degradation and bacterial community of sorghum-sudangrass silage. The results showed that the addition of LB significantly increased the pH and acetic acid content (p < 0.05), but high-quality silage was obtained. The addition of LBR and BS improved the fermentation quality of sorghum-sudangrass silage. The use of additives reduced the nitrate content in sorghum-sudangrass silage. The LB group increased the release of N2O at 3–7 days of ensiling (p < 0.05), and LBR and BS increased the release of N2O at 1–40 days of ensiling (p < 0.05). On the first day of ensiling, all silages were dominated by Weisslla, over 3 days of ensiling all silages were dominated by Lactobacillus. Acinetobacter, Serratia, Aquabacterium, and unclassified_f_enterobacteriaceae showed significant negative correlations with nitrate degradation during sorghum-sudangrass ensiling (p < 0.05). The BS and LBR groups increased the metabolic abundance of denitrification, dissimilatory nitrate reduction, and assimilatory nitrate reduction (p < 0.05). Overall, the additive ensures the fermentation quality of sorghum-sudangrass silage and promotes the degradation of nitrate by altering the bacterial community.https://www.frontiersin.org/articles/10.3389/fmicb.2022.1052837/fullsorghum-sudangrass silagefermentation qualitynitrateLactobacillus buchneriLactobacillus brevisBacillus subtilis |
| spellingShingle | Meirong Zhao Hongyu Zhang Gang Pan Hang Yin Juanjuan Sun Zhu Yu Chunsheng Bai Yanlin Xue Yanlin Xue Effect of exogenous microorganisms on the fermentation quality, nitrate degradation and bacterial community of sorghum-sudangrass silage Frontiers in Microbiology sorghum-sudangrass silage fermentation quality nitrate Lactobacillus buchneri Lactobacillus brevis Bacillus subtilis |
| title | Effect of exogenous microorganisms on the fermentation quality, nitrate degradation and bacterial community of sorghum-sudangrass silage |
| title_full | Effect of exogenous microorganisms on the fermentation quality, nitrate degradation and bacterial community of sorghum-sudangrass silage |
| title_fullStr | Effect of exogenous microorganisms on the fermentation quality, nitrate degradation and bacterial community of sorghum-sudangrass silage |
| title_full_unstemmed | Effect of exogenous microorganisms on the fermentation quality, nitrate degradation and bacterial community of sorghum-sudangrass silage |
| title_short | Effect of exogenous microorganisms on the fermentation quality, nitrate degradation and bacterial community of sorghum-sudangrass silage |
| title_sort | effect of exogenous microorganisms on the fermentation quality nitrate degradation and bacterial community of sorghum sudangrass silage |
| topic | sorghum-sudangrass silage fermentation quality nitrate Lactobacillus buchneri Lactobacillus brevis Bacillus subtilis |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1052837/full |
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