Diversity of Bacterial Biosynthetic Genes in Maritime Antarctica
Bacterial natural products (NPs) are still a major source of new drug leads. Polyketides (PKs) and non-ribosomal peptides (NRP) are two pharmaceutically important families of NPs and recent studies have revealed Antarctica to harbor endemic polyketide synthase (PKS) and non-ribosomal peptide synthet...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-02-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/2/279 |
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| author | Adriana Rego António G. G. Sousa João P. Santos Francisco Pascoal João Canário Pedro N. Leão Catarina Magalhães |
| author_facet | Adriana Rego António G. G. Sousa João P. Santos Francisco Pascoal João Canário Pedro N. Leão Catarina Magalhães |
| author_sort | Adriana Rego |
| collection | DOAJ |
| description | Bacterial natural products (NPs) are still a major source of new drug leads. Polyketides (PKs) and non-ribosomal peptides (NRP) are two pharmaceutically important families of NPs and recent studies have revealed Antarctica to harbor endemic polyketide synthase (PKS) and non-ribosomal peptide synthetase (NRPS) genes, likely to be involved in the production of novel metabolites. Despite this, the diversity of secondary metabolites genes in Antarctica is still poorly explored. In this study, a computational bioprospection approach was employed to study the diversity and identity of PKS and NRPS genes to one of the most biodiverse areas in maritime Antarctica—Maxwell Bay. Amplicon sequencing of soil samples targeting ketosynthase (KS) and adenylation (AD) domains of PKS and NRPS genes, respectively, revealed abundant and unexplored chemical diversity in this peninsula. About 20% of AD domain sequences were only distantly related to characterized biosynthetic genes. Several PKS and NRPS genes were found to be closely associated to recently described metabolites including those from uncultured and candidate phyla. The combination of new approaches in computational biology and new culture-dependent and -independent strategies is thus critical for the recovery of the potential novel chemistry encoded in Antarctica microorganisms. |
| first_indexed | 2024-04-13T16:11:58Z |
| format | Article |
| id | doaj.art-78509377ba7340d19af1c5b34efd9a98 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-04-13T16:11:58Z |
| publishDate | 2020-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-78509377ba7340d19af1c5b34efd9a982022-12-22T02:40:12ZengMDPI AGMicroorganisms2076-26072020-02-018227910.3390/microorganisms8020279microorganisms8020279Diversity of Bacterial Biosynthetic Genes in Maritime AntarcticaAdriana Rego0António G. G. Sousa1João P. Santos2Francisco Pascoal3João Canário4Pedro N. Leão5Catarina Magalhães6Interdisciplinary Centre of Marine and Environmental Research (CIIMAR), University of Porto, 4450-208 Matosinhos, PortugalInterdisciplinary Centre of Marine and Environmental Research (CIIMAR), University of Porto, 4450-208 Matosinhos, PortugalInterdisciplinary Centre of Marine and Environmental Research (CIIMAR), University of Porto, 4450-208 Matosinhos, PortugalInterdisciplinary Centre of Marine and Environmental Research (CIIMAR), University of Porto, 4450-208 Matosinhos, PortugalCentro de Química Estrutural at Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, PortugalInterdisciplinary Centre of Marine and Environmental Research (CIIMAR), University of Porto, 4450-208 Matosinhos, PortugalInterdisciplinary Centre of Marine and Environmental Research (CIIMAR), University of Porto, 4450-208 Matosinhos, PortugalBacterial natural products (NPs) are still a major source of new drug leads. Polyketides (PKs) and non-ribosomal peptides (NRP) are two pharmaceutically important families of NPs and recent studies have revealed Antarctica to harbor endemic polyketide synthase (PKS) and non-ribosomal peptide synthetase (NRPS) genes, likely to be involved in the production of novel metabolites. Despite this, the diversity of secondary metabolites genes in Antarctica is still poorly explored. In this study, a computational bioprospection approach was employed to study the diversity and identity of PKS and NRPS genes to one of the most biodiverse areas in maritime Antarctica—Maxwell Bay. Amplicon sequencing of soil samples targeting ketosynthase (KS) and adenylation (AD) domains of PKS and NRPS genes, respectively, revealed abundant and unexplored chemical diversity in this peninsula. About 20% of AD domain sequences were only distantly related to characterized biosynthetic genes. Several PKS and NRPS genes were found to be closely associated to recently described metabolites including those from uncultured and candidate phyla. The combination of new approaches in computational biology and new culture-dependent and -independent strategies is thus critical for the recovery of the potential novel chemistry encoded in Antarctica microorganisms.https://www.mdpi.com/2076-2607/8/2/279antarcticapolyketides (pks)non-ribosomal peptides (nrps)biosynthetic genescomputational bioprospectionketosynthase (ks)adenylation (ad)natural products (nps) |
| spellingShingle | Adriana Rego António G. G. Sousa João P. Santos Francisco Pascoal João Canário Pedro N. Leão Catarina Magalhães Diversity of Bacterial Biosynthetic Genes in Maritime Antarctica Microorganisms antarctica polyketides (pks) non-ribosomal peptides (nrps) biosynthetic genes computational bioprospection ketosynthase (ks) adenylation (ad) natural products (nps) |
| title | Diversity of Bacterial Biosynthetic Genes in Maritime Antarctica |
| title_full | Diversity of Bacterial Biosynthetic Genes in Maritime Antarctica |
| title_fullStr | Diversity of Bacterial Biosynthetic Genes in Maritime Antarctica |
| title_full_unstemmed | Diversity of Bacterial Biosynthetic Genes in Maritime Antarctica |
| title_short | Diversity of Bacterial Biosynthetic Genes in Maritime Antarctica |
| title_sort | diversity of bacterial biosynthetic genes in maritime antarctica |
| topic | antarctica polyketides (pks) non-ribosomal peptides (nrps) biosynthetic genes computational bioprospection ketosynthase (ks) adenylation (ad) natural products (nps) |
| url | https://www.mdpi.com/2076-2607/8/2/279 |
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