Development of a coronavirus disease 2019 nonhuman primate model using airborne exposure.
Airborne transmission is predicted to be a prevalent route of human exposure with SARS-CoV-2. Aside from African green monkeys, nonhuman primate models that replicate airborne transmission of SARS-CoV-2 have not been investigated. A comparative evaluation of COVID-19 in African green monkeys, rhesus...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2021-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0246366 |
| _version_ | 1819292874545561600 |
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| author | Sara C Johnston Keersten M Ricks Alexandra Jay Jo Lynne Raymond Franco Rossi Xiankun Zeng Jennifer Scruggs David Dyer Ondraya Frick Jeffrey W Koehler Paul A Kuehnert Tamara L Clements Charles J Shoemaker Susan R Coyne Korey L Delp Joshua Moore Kerry Berrier Heather Esham Joshua Shamblin Willie Sifford Jimmy Fiallos Leslie Klosterman Stephen Stevens Lauren White Philip Bowling Terrence Garcia Christopher Jensen Jeanean Ghering David Nyakiti Stephanie Bellanca Brian Kearney Wendy Giles Nazira Alli Fabian Paz Kristen Akers Denise Danner James Barth Joshua A Johnson Matthew Durant Ruth Kim Jay W Hooper Jeffrey M Smith Jeffrey R Kugelman Brett F Beitzel Kathleen M Gibson Margaret L M Pitt Timothy D Minogue Aysegul Nalca |
| author_facet | Sara C Johnston Keersten M Ricks Alexandra Jay Jo Lynne Raymond Franco Rossi Xiankun Zeng Jennifer Scruggs David Dyer Ondraya Frick Jeffrey W Koehler Paul A Kuehnert Tamara L Clements Charles J Shoemaker Susan R Coyne Korey L Delp Joshua Moore Kerry Berrier Heather Esham Joshua Shamblin Willie Sifford Jimmy Fiallos Leslie Klosterman Stephen Stevens Lauren White Philip Bowling Terrence Garcia Christopher Jensen Jeanean Ghering David Nyakiti Stephanie Bellanca Brian Kearney Wendy Giles Nazira Alli Fabian Paz Kristen Akers Denise Danner James Barth Joshua A Johnson Matthew Durant Ruth Kim Jay W Hooper Jeffrey M Smith Jeffrey R Kugelman Brett F Beitzel Kathleen M Gibson Margaret L M Pitt Timothy D Minogue Aysegul Nalca |
| author_sort | Sara C Johnston |
| collection | DOAJ |
| description | Airborne transmission is predicted to be a prevalent route of human exposure with SARS-CoV-2. Aside from African green monkeys, nonhuman primate models that replicate airborne transmission of SARS-CoV-2 have not been investigated. A comparative evaluation of COVID-19 in African green monkeys, rhesus macaques, and cynomolgus macaques following airborne exposure to SARS-CoV-2 was performed to determine critical disease parameters associated with disease progression, and establish correlations between primate and human COVID-19. Respiratory abnormalities and viral shedding were noted for all animals, indicating successful infection. Cynomolgus macaques developed fever, and thrombocytopenia was measured for African green monkeys and rhesus macaques. Type II pneumocyte hyperplasia and alveolar fibrosis were more frequently observed in lung tissue from cynomolgus macaques and African green monkeys. The data indicate that, in addition to African green monkeys, macaques can be successfully infected by airborne SARS-CoV-2, providing viable macaque natural transmission models for medical countermeasure evaluation. |
| first_indexed | 2024-12-24T04:01:28Z |
| format | Article |
| id | doaj.art-785181030e4e4293b32c07a9d4631b9b |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-24T04:01:28Z |
| publishDate | 2021-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-785181030e4e4293b32c07a9d4631b9b2022-12-21T17:16:19ZengPublic Library of Science (PLoS)PLoS ONE1932-62032021-01-01162e024636610.1371/journal.pone.0246366Development of a coronavirus disease 2019 nonhuman primate model using airborne exposure.Sara C JohnstonKeersten M RicksAlexandra JayJo Lynne RaymondFranco RossiXiankun ZengJennifer ScruggsDavid DyerOndraya FrickJeffrey W KoehlerPaul A KuehnertTamara L ClementsCharles J ShoemakerSusan R CoyneKorey L DelpJoshua MooreKerry BerrierHeather EshamJoshua ShamblinWillie SiffordJimmy FiallosLeslie KlostermanStephen StevensLauren WhitePhilip BowlingTerrence GarciaChristopher JensenJeanean GheringDavid NyakitiStephanie BellancaBrian KearneyWendy GilesNazira AlliFabian PazKristen AkersDenise DannerJames BarthJoshua A JohnsonMatthew DurantRuth KimJay W HooperJeffrey M SmithJeffrey R KugelmanBrett F BeitzelKathleen M GibsonMargaret L M PittTimothy D MinogueAysegul NalcaAirborne transmission is predicted to be a prevalent route of human exposure with SARS-CoV-2. Aside from African green monkeys, nonhuman primate models that replicate airborne transmission of SARS-CoV-2 have not been investigated. A comparative evaluation of COVID-19 in African green monkeys, rhesus macaques, and cynomolgus macaques following airborne exposure to SARS-CoV-2 was performed to determine critical disease parameters associated with disease progression, and establish correlations between primate and human COVID-19. Respiratory abnormalities and viral shedding were noted for all animals, indicating successful infection. Cynomolgus macaques developed fever, and thrombocytopenia was measured for African green monkeys and rhesus macaques. Type II pneumocyte hyperplasia and alveolar fibrosis were more frequently observed in lung tissue from cynomolgus macaques and African green monkeys. The data indicate that, in addition to African green monkeys, macaques can be successfully infected by airborne SARS-CoV-2, providing viable macaque natural transmission models for medical countermeasure evaluation.https://doi.org/10.1371/journal.pone.0246366 |
| spellingShingle | Sara C Johnston Keersten M Ricks Alexandra Jay Jo Lynne Raymond Franco Rossi Xiankun Zeng Jennifer Scruggs David Dyer Ondraya Frick Jeffrey W Koehler Paul A Kuehnert Tamara L Clements Charles J Shoemaker Susan R Coyne Korey L Delp Joshua Moore Kerry Berrier Heather Esham Joshua Shamblin Willie Sifford Jimmy Fiallos Leslie Klosterman Stephen Stevens Lauren White Philip Bowling Terrence Garcia Christopher Jensen Jeanean Ghering David Nyakiti Stephanie Bellanca Brian Kearney Wendy Giles Nazira Alli Fabian Paz Kristen Akers Denise Danner James Barth Joshua A Johnson Matthew Durant Ruth Kim Jay W Hooper Jeffrey M Smith Jeffrey R Kugelman Brett F Beitzel Kathleen M Gibson Margaret L M Pitt Timothy D Minogue Aysegul Nalca Development of a coronavirus disease 2019 nonhuman primate model using airborne exposure. PLoS ONE |
| title | Development of a coronavirus disease 2019 nonhuman primate model using airborne exposure. |
| title_full | Development of a coronavirus disease 2019 nonhuman primate model using airborne exposure. |
| title_fullStr | Development of a coronavirus disease 2019 nonhuman primate model using airborne exposure. |
| title_full_unstemmed | Development of a coronavirus disease 2019 nonhuman primate model using airborne exposure. |
| title_short | Development of a coronavirus disease 2019 nonhuman primate model using airborne exposure. |
| title_sort | development of a coronavirus disease 2019 nonhuman primate model using airborne exposure |
| url | https://doi.org/10.1371/journal.pone.0246366 |
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