Glucose Biosensor Based on Glucose Oxidase Immobilized on BSA Cross-Linked Nanocomposite Modified Glassy Carbon Electrode
Glucose sensors based blood glucose detection are of great significance for the diagnosis and treatment of diabetes because diabetes has aroused wide concern in the world. In this study, bovine serum albumin (BSA) was used to cross-link glucose oxidase (GOD) on a glassy carbon electrode (GCE) modifi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/23/6/3209 |
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| author | Yang-Yang Li Xin-Xin Ma Xin-Yan Song Lin-Lin Ma Yu-Ying Li Xin Meng Yu-Jie Chen Ke-Xin Xu Ali Akbar Moosavi-Movahedi Bao-Lin Xiao Jun Hong |
| author_facet | Yang-Yang Li Xin-Xin Ma Xin-Yan Song Lin-Lin Ma Yu-Ying Li Xin Meng Yu-Jie Chen Ke-Xin Xu Ali Akbar Moosavi-Movahedi Bao-Lin Xiao Jun Hong |
| author_sort | Yang-Yang Li |
| collection | DOAJ |
| description | Glucose sensors based blood glucose detection are of great significance for the diagnosis and treatment of diabetes because diabetes has aroused wide concern in the world. In this study, bovine serum albumin (BSA) was used to cross-link glucose oxidase (GOD) on a glassy carbon electrode (GCE) modified by a composite of hydroxy fullerene (HFs) and multi-walled carbon nanotubes (MWCNTs) and protected with a glutaraldehyde (GLA)/Nafion (NF) composite membrane to prepare a novel glucose biosensor. The modified materials were analyzed by UV-visible spectroscopy (UV-vis), transmission electron microscopy (TEM), and cyclic voltammetry (CV). The prepared MWCNTs-HFs composite has excellent conductivity, the addition of BSA regulates MWCNTs-HFs hydrophobicity and biocompatibility, and better immobilizes GOD on MWCNTs-HFs. MWCNTs-BSA-HFs plays a synergistic role in the electrochemical response to glucose. The biosensor shows high sensitivity (167 μA·mM<sup>−1</sup>·cm<sup>−2</sup>), wide calibration range (0.01–3.5 mM), and low detection limit (17 μM). The apparent Michaelis–Menten constant <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><msubsup><mi mathvariant="normal">K</mi><mi mathvariant="normal">m</mi><mrow><mi>app</mi></mrow></msubsup></mrow></semantics></math></inline-formula> is 119 μM. Additionally, the proposed biosensor has good selectivity and excellent storage stability (120 days). The practicability of the biosensor was evaluated in real plasma samples, and the recovery rate was satisfactory. |
| first_indexed | 2024-03-11T05:55:22Z |
| format | Article |
| id | doaj.art-785378d3c7524d94b53cd6f4ca189ce9 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-11T05:55:22Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-785378d3c7524d94b53cd6f4ca189ce92023-11-17T13:47:29ZengMDPI AGSensors1424-82202023-03-01236320910.3390/s23063209Glucose Biosensor Based on Glucose Oxidase Immobilized on BSA Cross-Linked Nanocomposite Modified Glassy Carbon ElectrodeYang-Yang Li0Xin-Xin Ma1Xin-Yan Song2Lin-Lin Ma3Yu-Ying Li4Xin Meng5Yu-Jie Chen6Ke-Xin Xu7Ali Akbar Moosavi-Movahedi8Bao-Lin Xiao9Jun Hong10School of Life Sciences, Henan University, Kaifeng 475000, ChinaSchool of Life Sciences, Henan University, Kaifeng 475000, ChinaSchool of Life Sciences, Henan University, Kaifeng 475000, ChinaSchool of Life Sciences, Henan University, Kaifeng 475000, ChinaSchool of Life Sciences, Henan University, Kaifeng 475000, ChinaSchool of Life Sciences, Henan University, Kaifeng 475000, ChinaSchool of Life Sciences, Henan University, Kaifeng 475000, ChinaSchool of Life Sciences, Henan University, Kaifeng 475000, ChinaInstitute of Biochemistry and Biophysics, University of Tehran, Tehran 1417614418, IranSchool of Life Sciences, Henan University, Kaifeng 475000, ChinaSchool of Life Sciences, Henan University, Kaifeng 475000, ChinaGlucose sensors based blood glucose detection are of great significance for the diagnosis and treatment of diabetes because diabetes has aroused wide concern in the world. In this study, bovine serum albumin (BSA) was used to cross-link glucose oxidase (GOD) on a glassy carbon electrode (GCE) modified by a composite of hydroxy fullerene (HFs) and multi-walled carbon nanotubes (MWCNTs) and protected with a glutaraldehyde (GLA)/Nafion (NF) composite membrane to prepare a novel glucose biosensor. The modified materials were analyzed by UV-visible spectroscopy (UV-vis), transmission electron microscopy (TEM), and cyclic voltammetry (CV). The prepared MWCNTs-HFs composite has excellent conductivity, the addition of BSA regulates MWCNTs-HFs hydrophobicity and biocompatibility, and better immobilizes GOD on MWCNTs-HFs. MWCNTs-BSA-HFs plays a synergistic role in the electrochemical response to glucose. The biosensor shows high sensitivity (167 μA·mM<sup>−1</sup>·cm<sup>−2</sup>), wide calibration range (0.01–3.5 mM), and low detection limit (17 μM). The apparent Michaelis–Menten constant <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><msubsup><mi mathvariant="normal">K</mi><mi mathvariant="normal">m</mi><mrow><mi>app</mi></mrow></msubsup></mrow></semantics></math></inline-formula> is 119 μM. Additionally, the proposed biosensor has good selectivity and excellent storage stability (120 days). The practicability of the biosensor was evaluated in real plasma samples, and the recovery rate was satisfactory.https://www.mdpi.com/1424-8220/23/6/3209glucose oxidasebiosensorhydroxyl fullerenesmulti-walled carbon nanotubesbovine serum albumin |
| spellingShingle | Yang-Yang Li Xin-Xin Ma Xin-Yan Song Lin-Lin Ma Yu-Ying Li Xin Meng Yu-Jie Chen Ke-Xin Xu Ali Akbar Moosavi-Movahedi Bao-Lin Xiao Jun Hong Glucose Biosensor Based on Glucose Oxidase Immobilized on BSA Cross-Linked Nanocomposite Modified Glassy Carbon Electrode Sensors glucose oxidase biosensor hydroxyl fullerenes multi-walled carbon nanotubes bovine serum albumin |
| title | Glucose Biosensor Based on Glucose Oxidase Immobilized on BSA Cross-Linked Nanocomposite Modified Glassy Carbon Electrode |
| title_full | Glucose Biosensor Based on Glucose Oxidase Immobilized on BSA Cross-Linked Nanocomposite Modified Glassy Carbon Electrode |
| title_fullStr | Glucose Biosensor Based on Glucose Oxidase Immobilized on BSA Cross-Linked Nanocomposite Modified Glassy Carbon Electrode |
| title_full_unstemmed | Glucose Biosensor Based on Glucose Oxidase Immobilized on BSA Cross-Linked Nanocomposite Modified Glassy Carbon Electrode |
| title_short | Glucose Biosensor Based on Glucose Oxidase Immobilized on BSA Cross-Linked Nanocomposite Modified Glassy Carbon Electrode |
| title_sort | glucose biosensor based on glucose oxidase immobilized on bsa cross linked nanocomposite modified glassy carbon electrode |
| topic | glucose oxidase biosensor hydroxyl fullerenes multi-walled carbon nanotubes bovine serum albumin |
| url | https://www.mdpi.com/1424-8220/23/6/3209 |
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