Culturable Diversity of Thraustochytrids from Coastal Waters of Qingdao and Their Fatty Acids
Thraustochytrids have gained significant attention in recent years because of their considerable ecological and biotechnological importance. Yet, the influence of seasons and habitats on their culturable diversity and lipid profile remains poorly described. In this study, a total of 58 thraustochytr...
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MDPI AG
2022-03-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/20/4/229 |
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| author | Mohan Bai Biswarup Sen Shuai Wen Huike Ye Yaodong He Xiaobo Zhang Guangyi Wang |
| author_facet | Mohan Bai Biswarup Sen Shuai Wen Huike Ye Yaodong He Xiaobo Zhang Guangyi Wang |
| author_sort | Mohan Bai |
| collection | DOAJ |
| description | Thraustochytrids have gained significant attention in recent years because of their considerable ecological and biotechnological importance. Yet, the influence of seasons and habitats on their culturable diversity and lipid profile remains poorly described. In this study, a total of 58 thraustochytrid strains were isolated from the coastal waters of Qingdao, China. These strains were phylogenetically close to five thraustochytrid genera, namely <i>Botryochytrium</i>, <i>Oblongichytrium</i>, <i>Schizochytrium</i>, <i>Thraustochytrium</i>, and <i>Sicyoidochytrium</i>. Most of the isolated strains were classified into the genera <i>Thraustochytrium</i> and <i>Oblongichytrium</i>. Further diversity analysis revealed that samples collected from nutrient-rich habitats and during summer/fall yielded significantly higher culturable diversity of thraustochytrids than those from low-nutrient habitats and winter/spring. Moreover, sampling habitats and seasons significantly impacted the fatty acid profiles of the strains. Particularly, the <i>Oblongichytrium</i> sp. OC931 strain produced a significant amount (153.99 mg/L) of eicosapentaenoic acid (EPA), accounting for 9.12% of the total fatty acids, which was significantly higher than that of the previously reported <i>Aurantiochytrium</i> strains. Overall, the results of this study fill the gap in our current understanding of the culturable diversity of thraustochytrids in the coastal waters and the impact of the sampling habitats and seasons on their capacity for lipid accumulation. |
| first_indexed | 2024-03-09T13:23:25Z |
| format | Article |
| id | doaj.art-78568d5152a24629a9b4a9ff8d914b66 |
| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-09T13:23:25Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-78568d5152a24629a9b4a9ff8d914b662023-11-30T21:26:46ZengMDPI AGMarine Drugs1660-33972022-03-0120422910.3390/md20040229Culturable Diversity of Thraustochytrids from Coastal Waters of Qingdao and Their Fatty AcidsMohan Bai0Biswarup Sen1Shuai Wen2Huike Ye3Yaodong He4Xiaobo Zhang5Guangyi Wang6College of Life Sciences, Zijingang Campus, Zhejiang University, Hangzhou 310058, ChinaCenter for Marine Environmental Ecology, School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, ChinaCenter for Marine Environmental Ecology, School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, ChinaCenter for Marine Environmental Ecology, School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, ChinaCenter for Marine Environmental Ecology, School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, ChinaCollege of Life Sciences, Zijingang Campus, Zhejiang University, Hangzhou 310058, ChinaCenter for Marine Environmental Ecology, School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, ChinaThraustochytrids have gained significant attention in recent years because of their considerable ecological and biotechnological importance. Yet, the influence of seasons and habitats on their culturable diversity and lipid profile remains poorly described. In this study, a total of 58 thraustochytrid strains were isolated from the coastal waters of Qingdao, China. These strains were phylogenetically close to five thraustochytrid genera, namely <i>Botryochytrium</i>, <i>Oblongichytrium</i>, <i>Schizochytrium</i>, <i>Thraustochytrium</i>, and <i>Sicyoidochytrium</i>. Most of the isolated strains were classified into the genera <i>Thraustochytrium</i> and <i>Oblongichytrium</i>. Further diversity analysis revealed that samples collected from nutrient-rich habitats and during summer/fall yielded significantly higher culturable diversity of thraustochytrids than those from low-nutrient habitats and winter/spring. Moreover, sampling habitats and seasons significantly impacted the fatty acid profiles of the strains. Particularly, the <i>Oblongichytrium</i> sp. OC931 strain produced a significant amount (153.99 mg/L) of eicosapentaenoic acid (EPA), accounting for 9.12% of the total fatty acids, which was significantly higher than that of the previously reported <i>Aurantiochytrium</i> strains. Overall, the results of this study fill the gap in our current understanding of the culturable diversity of thraustochytrids in the coastal waters and the impact of the sampling habitats and seasons on their capacity for lipid accumulation.https://www.mdpi.com/1660-3397/20/4/229thraustochytridsdiversitybiomassfatty acidsseasonshabitats |
| spellingShingle | Mohan Bai Biswarup Sen Shuai Wen Huike Ye Yaodong He Xiaobo Zhang Guangyi Wang Culturable Diversity of Thraustochytrids from Coastal Waters of Qingdao and Their Fatty Acids Marine Drugs thraustochytrids diversity biomass fatty acids seasons habitats |
| title | Culturable Diversity of Thraustochytrids from Coastal Waters of Qingdao and Their Fatty Acids |
| title_full | Culturable Diversity of Thraustochytrids from Coastal Waters of Qingdao and Their Fatty Acids |
| title_fullStr | Culturable Diversity of Thraustochytrids from Coastal Waters of Qingdao and Their Fatty Acids |
| title_full_unstemmed | Culturable Diversity of Thraustochytrids from Coastal Waters of Qingdao and Their Fatty Acids |
| title_short | Culturable Diversity of Thraustochytrids from Coastal Waters of Qingdao and Their Fatty Acids |
| title_sort | culturable diversity of thraustochytrids from coastal waters of qingdao and their fatty acids |
| topic | thraustochytrids diversity biomass fatty acids seasons habitats |
| url | https://www.mdpi.com/1660-3397/20/4/229 |
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