Genetic Diversity of <i>Plasmodium vivax</i> Cysteine-Rich Protective Antigen (PvCyRPA) in Field Isolates from Five Different Areas of the Brazilian Amazon
The <i>Plasmodium vivax</i> Cysteine-Rich Protective Antigen (PvCyRPA) has an important role in erythrocyte invasion and has been considered a target for <i>vivax</i> malaria vaccine development. Nonetheless, its genetic diversity remains uncharted in Brazilian malaria-endemi...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-10-01
|
| Series: | Genes |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4425/12/11/1657 |
| _version_ | 1797510227308838912 |
|---|---|
| author | Lana Bitencourt Chaves Glaucia de Oliveira Guimarães Daiana de Souza Perce-da-Silva Dalma Maria Banic Paulo Renato Rivas Totino Ricardo Luiz Dantas Machado Rodrigo Nunes Rodrigues-da-Silva Lilian Rose Pratt-Riccio Cláudio Tadeu Daniel-Ribeiro Josué da Costa Lima-Junior |
| author_facet | Lana Bitencourt Chaves Glaucia de Oliveira Guimarães Daiana de Souza Perce-da-Silva Dalma Maria Banic Paulo Renato Rivas Totino Ricardo Luiz Dantas Machado Rodrigo Nunes Rodrigues-da-Silva Lilian Rose Pratt-Riccio Cláudio Tadeu Daniel-Ribeiro Josué da Costa Lima-Junior |
| author_sort | Lana Bitencourt Chaves |
| collection | DOAJ |
| description | The <i>Plasmodium vivax</i> Cysteine-Rich Protective Antigen (PvCyRPA) has an important role in erythrocyte invasion and has been considered a target for <i>vivax</i> malaria vaccine development. Nonetheless, its genetic diversity remains uncharted in Brazilian malaria-endemic areas. Therefore, we investigated the <i>pvcyrpa</i> genetic polymorphism in 98 field isolates from the Brazilian Amazon and its impact on the antigenicity of predicted B-cell epitopes. Genetic diversity parameters, population genetic analysis, neutrality test and the median-joining network were analyzed, and the potential amino acid polymorphism participation in B-cell epitopes was investigated. One synonymous and 26 non-synonymous substitutions defined fifty haplotypes. The nucleotide diversity and Tajima’s D values varied across the coding gene. The exon-1 sequence had greater diversity than those of exon-2. Concerning the prediction analysis, seven sequences were predicted as linear B cell epitopes, the majority contained in conformational epitopes. Moreover, important amino acid polymorphism was detected in regions predicted to contain residues participating in B-cell epitopes. Our data suggest that the <i>pvcyrpa</i> gene presents a moderate polymorphism in the studied isolates and such polymorphisms alter amino acid sequences contained in potential B cell epitopes, an important observation considering the antigen potentiality as a vaccine candidate to cover distinct <i>P. vivax</i> endemic areas worldwide. |
| first_indexed | 2024-03-10T05:28:36Z |
| format | Article |
| id | doaj.art-785ae10b291b48e89812569d70ae83de |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-10T05:28:36Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-785ae10b291b48e89812569d70ae83de2023-11-22T23:26:53ZengMDPI AGGenes2073-44252021-10-011211165710.3390/genes12111657Genetic Diversity of <i>Plasmodium vivax</i> Cysteine-Rich Protective Antigen (PvCyRPA) in Field Isolates from Five Different Areas of the Brazilian AmazonLana Bitencourt Chaves0Glaucia de Oliveira Guimarães1Daiana de Souza Perce-da-Silva2Dalma Maria Banic3Paulo Renato Rivas Totino4Ricardo Luiz Dantas Machado5Rodrigo Nunes Rodrigues-da-Silva6Lilian Rose Pratt-Riccio7Cláudio Tadeu Daniel-Ribeiro8Josué da Costa Lima-Junior9Laboratory of Immunoparasitology, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro 21040-900, BrazilLaboratory of Immunoparasitology, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro 21040-900, BrazilLaboratory of Basic and Applied Immunology, Arthur Sá Earp Neto University Center, Petrópolis 25680-120, BrazilLaboratory of Clinical Immunology, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro 21040-900, BrazilLaboratory of Malaria Research, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro 21040-900, BrazilDepartment of Microbiology and Parasitology, Biomedical Institute, Fluminense Federal University, Rio de Janeiro 24210-130, BrazilLaboratory of Monoclonal Antibodies Technology, Institute of Immunobiology Technology, Fiocruz, Rio de Janeiro 21040-900, BrazilLaboratory of Malaria Research, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro 21040-900, BrazilLaboratory of Malaria Research, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro 21040-900, BrazilLaboratory of Immunoparasitology, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro 21040-900, BrazilThe <i>Plasmodium vivax</i> Cysteine-Rich Protective Antigen (PvCyRPA) has an important role in erythrocyte invasion and has been considered a target for <i>vivax</i> malaria vaccine development. Nonetheless, its genetic diversity remains uncharted in Brazilian malaria-endemic areas. Therefore, we investigated the <i>pvcyrpa</i> genetic polymorphism in 98 field isolates from the Brazilian Amazon and its impact on the antigenicity of predicted B-cell epitopes. Genetic diversity parameters, population genetic analysis, neutrality test and the median-joining network were analyzed, and the potential amino acid polymorphism participation in B-cell epitopes was investigated. One synonymous and 26 non-synonymous substitutions defined fifty haplotypes. The nucleotide diversity and Tajima’s D values varied across the coding gene. The exon-1 sequence had greater diversity than those of exon-2. Concerning the prediction analysis, seven sequences were predicted as linear B cell epitopes, the majority contained in conformational epitopes. Moreover, important amino acid polymorphism was detected in regions predicted to contain residues participating in B-cell epitopes. Our data suggest that the <i>pvcyrpa</i> gene presents a moderate polymorphism in the studied isolates and such polymorphisms alter amino acid sequences contained in potential B cell epitopes, an important observation considering the antigen potentiality as a vaccine candidate to cover distinct <i>P. vivax</i> endemic areas worldwide.https://www.mdpi.com/2073-4425/12/11/1657malariaBrazilian Amazon<i>Plasmodium vivax</i>PvCyRPAgenetic diversityprediction |
| spellingShingle | Lana Bitencourt Chaves Glaucia de Oliveira Guimarães Daiana de Souza Perce-da-Silva Dalma Maria Banic Paulo Renato Rivas Totino Ricardo Luiz Dantas Machado Rodrigo Nunes Rodrigues-da-Silva Lilian Rose Pratt-Riccio Cláudio Tadeu Daniel-Ribeiro Josué da Costa Lima-Junior Genetic Diversity of <i>Plasmodium vivax</i> Cysteine-Rich Protective Antigen (PvCyRPA) in Field Isolates from Five Different Areas of the Brazilian Amazon Genes malaria Brazilian Amazon <i>Plasmodium vivax</i> PvCyRPA genetic diversity prediction |
| title | Genetic Diversity of <i>Plasmodium vivax</i> Cysteine-Rich Protective Antigen (PvCyRPA) in Field Isolates from Five Different Areas of the Brazilian Amazon |
| title_full | Genetic Diversity of <i>Plasmodium vivax</i> Cysteine-Rich Protective Antigen (PvCyRPA) in Field Isolates from Five Different Areas of the Brazilian Amazon |
| title_fullStr | Genetic Diversity of <i>Plasmodium vivax</i> Cysteine-Rich Protective Antigen (PvCyRPA) in Field Isolates from Five Different Areas of the Brazilian Amazon |
| title_full_unstemmed | Genetic Diversity of <i>Plasmodium vivax</i> Cysteine-Rich Protective Antigen (PvCyRPA) in Field Isolates from Five Different Areas of the Brazilian Amazon |
| title_short | Genetic Diversity of <i>Plasmodium vivax</i> Cysteine-Rich Protective Antigen (PvCyRPA) in Field Isolates from Five Different Areas of the Brazilian Amazon |
| title_sort | genetic diversity of i plasmodium vivax i cysteine rich protective antigen pvcyrpa in field isolates from five different areas of the brazilian amazon |
| topic | malaria Brazilian Amazon <i>Plasmodium vivax</i> PvCyRPA genetic diversity prediction |
| url | https://www.mdpi.com/2073-4425/12/11/1657 |
| work_keys_str_mv | AT lanabitencourtchaves geneticdiversityofiplasmodiumvivaxicysteinerichprotectiveantigenpvcyrpainfieldisolatesfromfivedifferentareasofthebrazilianamazon AT glauciadeoliveiraguimaraes geneticdiversityofiplasmodiumvivaxicysteinerichprotectiveantigenpvcyrpainfieldisolatesfromfivedifferentareasofthebrazilianamazon AT daianadesouzapercedasilva geneticdiversityofiplasmodiumvivaxicysteinerichprotectiveantigenpvcyrpainfieldisolatesfromfivedifferentareasofthebrazilianamazon AT dalmamariabanic geneticdiversityofiplasmodiumvivaxicysteinerichprotectiveantigenpvcyrpainfieldisolatesfromfivedifferentareasofthebrazilianamazon AT paulorenatorivastotino geneticdiversityofiplasmodiumvivaxicysteinerichprotectiveantigenpvcyrpainfieldisolatesfromfivedifferentareasofthebrazilianamazon AT ricardoluizdantasmachado geneticdiversityofiplasmodiumvivaxicysteinerichprotectiveantigenpvcyrpainfieldisolatesfromfivedifferentareasofthebrazilianamazon AT rodrigonunesrodriguesdasilva geneticdiversityofiplasmodiumvivaxicysteinerichprotectiveantigenpvcyrpainfieldisolatesfromfivedifferentareasofthebrazilianamazon AT lilianroseprattriccio geneticdiversityofiplasmodiumvivaxicysteinerichprotectiveantigenpvcyrpainfieldisolatesfromfivedifferentareasofthebrazilianamazon AT claudiotadeudanielribeiro geneticdiversityofiplasmodiumvivaxicysteinerichprotectiveantigenpvcyrpainfieldisolatesfromfivedifferentareasofthebrazilianamazon AT josuedacostalimajunior geneticdiversityofiplasmodiumvivaxicysteinerichprotectiveantigenpvcyrpainfieldisolatesfromfivedifferentareasofthebrazilianamazon |