Characterization of transcription factor MYB59 and expression profiling in response to low K+ and NO3 − in indica rice (Oryza sativa L.)
Abstract Background Nitrogen and potassium are crucial supplements for plant development and growth. Plants can detect potassium and nitrate ions in soils and in like way, they modify root-to-shoot transport of these ions to adjust the conveyance among roots and shoots. Transcription factor MYB59 pl...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-10-01
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| Series: | Journal of Genetic Engineering and Biotechnology |
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| Online Access: | https://doi.org/10.1186/s43141-021-00248-6 |
| _version_ | 1797203850757668864 |
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| author | Md. Qamrul Islam Md. Nazmul Hasan Hammadul Hoque Nurnabi Azad Jewel Md. Fahmid Hossain Bhuiyan Shamsul H. Prodhan |
| author_facet | Md. Qamrul Islam Md. Nazmul Hasan Hammadul Hoque Nurnabi Azad Jewel Md. Fahmid Hossain Bhuiyan Shamsul H. Prodhan |
| author_sort | Md. Qamrul Islam |
| collection | DOAJ |
| description | Abstract Background Nitrogen and potassium are crucial supplements for plant development and growth. Plants can detect potassium and nitrate ions in soils and in like way, they modify root-to-shoot transport of these ions to adjust the conveyance among roots and shoots. Transcription factor MYB59 plays essential roles in numerous physiological processes inclusive of hormone response, abiotic stress tolerance, plant development, and metabolic regulation. In this study, we retrieved 56 MYB59 proteins from different plant species. Multiple sequence alignment, phylogenetic tree, conserved motif, chromosomal localization, and cis-regulatory elements of the retrieved sequences were analyzed. Gene structure, protein 3D structure, and DNA binding of OsMYB59 indica were also predicted. Finally, we characterized OsMYB59 and its function under low K+/NO3 − conditions in Oryza sativa subsp. indica. Results Data analysis showed that MYB59s from various groups separated in terms of conserved functional domains and gene structure, where members of genus Oryza clustered together. Plants showed reduced height and yellowish appearance when grown on K+ and NO3 − deficient medium. Quantitative real-time PCR uncovered that the OsMYB59 reacted to abiotic stresses where its expression was increased in BRRI dhan56 but decreased in other varieties on K+ deficient medium. In addition, OsMYB59 transcript level increased on NO3 − deficient medium. Conclusions Our results can help to explain the biological functions of indica rice MYB59 protein and gave a theoretical premise to additionally describe its biological roles in response to abiotic stresses particularly drought. |
| first_indexed | 2024-04-24T08:25:53Z |
| format | Article |
| id | doaj.art-785be1ba642141c3b71b812d108b4b18 |
| institution | Directory Open Access Journal |
| issn | 2090-5920 |
| language | English |
| last_indexed | 2024-04-24T08:25:53Z |
| publishDate | 2021-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Genetic Engineering and Biotechnology |
| spelling | doaj.art-785be1ba642141c3b71b812d108b4b182024-04-16T22:24:01ZengElsevierJournal of Genetic Engineering and Biotechnology2090-59202021-10-0119111410.1186/s43141-021-00248-6Characterization of transcription factor MYB59 and expression profiling in response to low K+ and NO3 − in indica rice (Oryza sativa L.)Md. Qamrul Islam0Md. Nazmul Hasan1Hammadul Hoque2Nurnabi Azad Jewel3Md. Fahmid Hossain Bhuiyan4Shamsul H. Prodhan5Department of Genetic Engineering and Biotechnology, Shahjalal University of Science and TechnologyDepartment of Genetic Engineering and Biotechnology, Shahjalal University of Science and TechnologyDepartment of Genetic Engineering and Biotechnology, Shahjalal University of Science and TechnologyDepartment of Genetic Engineering and Biotechnology, Shahjalal University of Science and TechnologyDepartment of Genetic Engineering and Biotechnology, Shahjalal University of Science and TechnologyDepartment of Genetic Engineering and Biotechnology, Shahjalal University of Science and TechnologyAbstract Background Nitrogen and potassium are crucial supplements for plant development and growth. Plants can detect potassium and nitrate ions in soils and in like way, they modify root-to-shoot transport of these ions to adjust the conveyance among roots and shoots. Transcription factor MYB59 plays essential roles in numerous physiological processes inclusive of hormone response, abiotic stress tolerance, plant development, and metabolic regulation. In this study, we retrieved 56 MYB59 proteins from different plant species. Multiple sequence alignment, phylogenetic tree, conserved motif, chromosomal localization, and cis-regulatory elements of the retrieved sequences were analyzed. Gene structure, protein 3D structure, and DNA binding of OsMYB59 indica were also predicted. Finally, we characterized OsMYB59 and its function under low K+/NO3 − conditions in Oryza sativa subsp. indica. Results Data analysis showed that MYB59s from various groups separated in terms of conserved functional domains and gene structure, where members of genus Oryza clustered together. Plants showed reduced height and yellowish appearance when grown on K+ and NO3 − deficient medium. Quantitative real-time PCR uncovered that the OsMYB59 reacted to abiotic stresses where its expression was increased in BRRI dhan56 but decreased in other varieties on K+ deficient medium. In addition, OsMYB59 transcript level increased on NO3 − deficient medium. Conclusions Our results can help to explain the biological functions of indica rice MYB59 protein and gave a theoretical premise to additionally describe its biological roles in response to abiotic stresses particularly drought.https://doi.org/10.1186/s43141-021-00248-6PotassiumNitrogenTranscription factorPhylogenyOsMYB593D structure |
| spellingShingle | Md. Qamrul Islam Md. Nazmul Hasan Hammadul Hoque Nurnabi Azad Jewel Md. Fahmid Hossain Bhuiyan Shamsul H. Prodhan Characterization of transcription factor MYB59 and expression profiling in response to low K+ and NO3 − in indica rice (Oryza sativa L.) Journal of Genetic Engineering and Biotechnology Potassium Nitrogen Transcription factor Phylogeny OsMYB59 3D structure |
| title | Characterization of transcription factor MYB59 and expression profiling in response to low K+ and NO3 − in indica rice (Oryza sativa L.) |
| title_full | Characterization of transcription factor MYB59 and expression profiling in response to low K+ and NO3 − in indica rice (Oryza sativa L.) |
| title_fullStr | Characterization of transcription factor MYB59 and expression profiling in response to low K+ and NO3 − in indica rice (Oryza sativa L.) |
| title_full_unstemmed | Characterization of transcription factor MYB59 and expression profiling in response to low K+ and NO3 − in indica rice (Oryza sativa L.) |
| title_short | Characterization of transcription factor MYB59 and expression profiling in response to low K+ and NO3 − in indica rice (Oryza sativa L.) |
| title_sort | characterization of transcription factor myb59 and expression profiling in response to low k and no3 in indica rice oryza sativa l |
| topic | Potassium Nitrogen Transcription factor Phylogeny OsMYB59 3D structure |
| url | https://doi.org/10.1186/s43141-021-00248-6 |
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