Identification of Differentially Expressed Genes in the Longissimus Dorsi Muscle of Luchuan and Duroc Pigs by Transcriptome Sequencing
The Duroc pig originated in the United States and is a typical lean-meat pig. The breed grows fast, and the body size is large, but the meat quality is poor. The Luchuan pig is one of eight excellent local breeds in China; it has tender meat but is small in size. To study the factors that determine...
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MDPI AG
2023-01-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/14/1/132 |
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| author | Pengcheng Pan Zhaoxian Qin Wan Xie Baojian Chen Zhihui Guan Bingkun Xie |
| author_facet | Pengcheng Pan Zhaoxian Qin Wan Xie Baojian Chen Zhihui Guan Bingkun Xie |
| author_sort | Pengcheng Pan |
| collection | DOAJ |
| description | The Duroc pig originated in the United States and is a typical lean-meat pig. The breed grows fast, and the body size is large, but the meat quality is poor. The Luchuan pig is one of eight excellent local breeds in China; it has tender meat but is small in size. To study the factors that determine growth, we selected the longissimus dorsi muscle of Luchuan and Duroc pigs for transcriptome sequencing. The results of the transcriptome showed that 3682 genes were differentially expressed (DEGs) in the longissimus dorsi muscle of Duroc and Luchuan pigs. We screened out genes related to muscle development and selected the MYL2 (Myosin light chain-2) gene to perform preliminary research. Gene Ontology (GO) enrichment of biological functions and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that the gene products were mainly involved in the Akt/FoxO signaling pathway, fatty acid metabolism, arachidonic acid metabolism and glycine, serine and threonine metabolism. Such pathways contributed to skeletal muscle growth, fatty acid metabolism and intramuscular fat deposition. These results provide insight into the mechanisms underlying the formation of skeletal muscle and provide candidate genes to improve growth traits, as well as contribute to improving the growth and development traits of pigs through molecular breeding. |
| first_indexed | 2024-03-09T12:36:01Z |
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| id | doaj.art-785e08ec3d4d43899101497e8e0e34b8 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-09T12:36:01Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-785e08ec3d4d43899101497e8e0e34b82023-11-30T22:24:02ZengMDPI AGGenes2073-44252023-01-0114113210.3390/genes14010132Identification of Differentially Expressed Genes in the Longissimus Dorsi Muscle of Luchuan and Duroc Pigs by Transcriptome SequencingPengcheng Pan0Zhaoxian Qin1Wan Xie2Baojian Chen3Zhihui Guan4Bingkun Xie5Guangxi Key Laboratory of Livestock Genetic Improvement, Guangxi Agricultural Vocational and Technical University, Nanning 530001, ChinaGuangxi Key Laboratory of Livestock Genetic Improvement, Guangxi Agricultural Vocational and Technical University, Nanning 530001, ChinaCollege of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, ChinaGuangxi Key Laboratory of Livestock Genetic Improvement, Guangxi Agricultural Vocational and Technical University, Nanning 530001, ChinaGuangxi Key Laboratory of Livestock Genetic Improvement, Guangxi Agricultural Vocational and Technical University, Nanning 530001, ChinaGuangxi Key Laboratory of Livestock Genetic Improvement, Guangxi Agricultural Vocational and Technical University, Nanning 530001, ChinaThe Duroc pig originated in the United States and is a typical lean-meat pig. The breed grows fast, and the body size is large, but the meat quality is poor. The Luchuan pig is one of eight excellent local breeds in China; it has tender meat but is small in size. To study the factors that determine growth, we selected the longissimus dorsi muscle of Luchuan and Duroc pigs for transcriptome sequencing. The results of the transcriptome showed that 3682 genes were differentially expressed (DEGs) in the longissimus dorsi muscle of Duroc and Luchuan pigs. We screened out genes related to muscle development and selected the MYL2 (Myosin light chain-2) gene to perform preliminary research. Gene Ontology (GO) enrichment of biological functions and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that the gene products were mainly involved in the Akt/FoxO signaling pathway, fatty acid metabolism, arachidonic acid metabolism and glycine, serine and threonine metabolism. Such pathways contributed to skeletal muscle growth, fatty acid metabolism and intramuscular fat deposition. These results provide insight into the mechanisms underlying the formation of skeletal muscle and provide candidate genes to improve growth traits, as well as contribute to improving the growth and development traits of pigs through molecular breeding.https://www.mdpi.com/2073-4425/14/1/132growth and developmentLuchuan piglongissimus dorsi musclesmyosin light chain-2 (MYL2/MLC-2)transcriptomics |
| spellingShingle | Pengcheng Pan Zhaoxian Qin Wan Xie Baojian Chen Zhihui Guan Bingkun Xie Identification of Differentially Expressed Genes in the Longissimus Dorsi Muscle of Luchuan and Duroc Pigs by Transcriptome Sequencing Genes growth and development Luchuan pig longissimus dorsi muscles myosin light chain-2 (MYL2/MLC-2) transcriptomics |
| title | Identification of Differentially Expressed Genes in the Longissimus Dorsi Muscle of Luchuan and Duroc Pigs by Transcriptome Sequencing |
| title_full | Identification of Differentially Expressed Genes in the Longissimus Dorsi Muscle of Luchuan and Duroc Pigs by Transcriptome Sequencing |
| title_fullStr | Identification of Differentially Expressed Genes in the Longissimus Dorsi Muscle of Luchuan and Duroc Pigs by Transcriptome Sequencing |
| title_full_unstemmed | Identification of Differentially Expressed Genes in the Longissimus Dorsi Muscle of Luchuan and Duroc Pigs by Transcriptome Sequencing |
| title_short | Identification of Differentially Expressed Genes in the Longissimus Dorsi Muscle of Luchuan and Duroc Pigs by Transcriptome Sequencing |
| title_sort | identification of differentially expressed genes in the longissimus dorsi muscle of luchuan and duroc pigs by transcriptome sequencing |
| topic | growth and development Luchuan pig longissimus dorsi muscles myosin light chain-2 (MYL2/MLC-2) transcriptomics |
| url | https://www.mdpi.com/2073-4425/14/1/132 |
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