Controlled Assembly of Fluorophores inside a Nanoliposome
Cellular compartmentalization plays an essential role in organizing the complex and multiple biochemical reactions in the cell. An artificial compartment would provide powerful strategies to develop new biochemical tools for material production and diagnosis, but it is still a great challenge to syn...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-01-01
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| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/28/2/911 |
| _version_ | 1797438205846355968 |
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| author | Hiroaki Konishi Eiji Nakata Futa Komatsubara Takashi Morii |
| author_facet | Hiroaki Konishi Eiji Nakata Futa Komatsubara Takashi Morii |
| author_sort | Hiroaki Konishi |
| collection | DOAJ |
| description | Cellular compartmentalization plays an essential role in organizing the complex and multiple biochemical reactions in the cell. An artificial compartment would provide powerful strategies to develop new biochemical tools for material production and diagnosis, but it is still a great challenge to synthesize the compartments that encapsulate materials of interest while controlling their accurate locations, numbers, and stoichiometry. In this study, we evaluated chemical characteristics of a liposome-encapsulated compartment, which has great potential to locate various materials of interest with precise control of their locations and numbers in the compartment. A nanoliposome was constructed inside a ring-shaped DNA origami skeleton according to the method of Yang et al., and further equipped with a double-stranded DNA platform to assemble molecules of interest in the nanoliposome. Upon formation of the nanoliposome, a pH-sensitive fluorophore on the bridged platform showed little or no response to the pH change of the outer buffer, ensuring that the molecules assembled on the platform are effectively shielded from the outer environment. The ring-shaped DNA skeleton equipped with a double-stranded DNA platform allows spatial assembly of several functional molecules inside the nanoliposome to isolate them from the outer environment. |
| first_indexed | 2024-03-09T11:33:35Z |
| format | Article |
| id | doaj.art-785e2b2b01324353800b34c7d96da08f |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T11:33:35Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-785e2b2b01324353800b34c7d96da08f2023-11-30T23:46:43ZengMDPI AGMolecules1420-30492023-01-0128291110.3390/molecules28020911Controlled Assembly of Fluorophores inside a NanoliposomeHiroaki Konishi0Eiji Nakata1Futa Komatsubara2Takashi Morii3Institute of Advanced Energy, Kyoto University, Uji, Kyoto 611-011, JapanInstitute of Advanced Energy, Kyoto University, Uji, Kyoto 611-011, JapanInstitute of Advanced Energy, Kyoto University, Uji, Kyoto 611-011, JapanInstitute of Advanced Energy, Kyoto University, Uji, Kyoto 611-011, JapanCellular compartmentalization plays an essential role in organizing the complex and multiple biochemical reactions in the cell. An artificial compartment would provide powerful strategies to develop new biochemical tools for material production and diagnosis, but it is still a great challenge to synthesize the compartments that encapsulate materials of interest while controlling their accurate locations, numbers, and stoichiometry. In this study, we evaluated chemical characteristics of a liposome-encapsulated compartment, which has great potential to locate various materials of interest with precise control of their locations and numbers in the compartment. A nanoliposome was constructed inside a ring-shaped DNA origami skeleton according to the method of Yang et al., and further equipped with a double-stranded DNA platform to assemble molecules of interest in the nanoliposome. Upon formation of the nanoliposome, a pH-sensitive fluorophore on the bridged platform showed little or no response to the pH change of the outer buffer, ensuring that the molecules assembled on the platform are effectively shielded from the outer environment. The ring-shaped DNA skeleton equipped with a double-stranded DNA platform allows spatial assembly of several functional molecules inside the nanoliposome to isolate them from the outer environment.https://www.mdpi.com/1420-3049/28/2/911DNA nanostructureDNA origaminanoliposomecompartmentencapsulation |
| spellingShingle | Hiroaki Konishi Eiji Nakata Futa Komatsubara Takashi Morii Controlled Assembly of Fluorophores inside a Nanoliposome Molecules DNA nanostructure DNA origami nanoliposome compartment encapsulation |
| title | Controlled Assembly of Fluorophores inside a Nanoliposome |
| title_full | Controlled Assembly of Fluorophores inside a Nanoliposome |
| title_fullStr | Controlled Assembly of Fluorophores inside a Nanoliposome |
| title_full_unstemmed | Controlled Assembly of Fluorophores inside a Nanoliposome |
| title_short | Controlled Assembly of Fluorophores inside a Nanoliposome |
| title_sort | controlled assembly of fluorophores inside a nanoliposome |
| topic | DNA nanostructure DNA origami nanoliposome compartment encapsulation |
| url | https://www.mdpi.com/1420-3049/28/2/911 |
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