Ligustrazine Inhibits the Migration and Invasion of Renal Cell Carcinoma
Ligustrazine is a Chinese herb (Chuanxiong) approved for use as a medical drug in China. Recent evidence suggests that ligustrazine has promising antitumor properties. Our preliminary results showed that ligustrazine could inhibit the growth of human renal cell carcinoma (RCC) cell lines. However,...
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| Format: | Article |
| Language: | English |
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Codon Publications
2023-01-01
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| Series: | Journal of Kidney Cancer and VHL |
| Subjects: | |
| Online Access: | https://mail.jkcvhl.com/~jkcvhlco/index.php/jkcvhl/article/view/232 |
| _version_ | 1797844802036826112 |
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| author | Xufeng Zhang Junfu Wang Yanhua Cao Kalin Li Chao Sun Wen Jiang Qian Xin Jue Wang Tonggang Qi Shuangde Liu Yun Luan |
| author_facet | Xufeng Zhang Junfu Wang Yanhua Cao Kalin Li Chao Sun Wen Jiang Qian Xin Jue Wang Tonggang Qi Shuangde Liu Yun Luan |
| author_sort | Xufeng Zhang |
| collection | DOAJ |
| description |
Ligustrazine is a Chinese herb (Chuanxiong) approved for use as a medical drug in China. Recent evidence suggests that ligustrazine has promising antitumor properties. Our preliminary results showed that ligustrazine could inhibit the growth of human renal cell carcinoma (RCC) cell lines. However, the complicated molecular mechanism has not been fully revealed. Therefore, the purpose of this study to investigate the mechanism of ligustrazine resistance in human RCC cells. Cell proliferation, migration, invasion, and colony-formation ability of RCC cells A498 were detected by MTT assay, clonal formation rates, and transwell chamber assay in vitro. The expression of epithelial–mesenchymal transition (EMT)–related proteins were analyzed using western blot test. The effect of ligustrazine on the growth of A498 cells in nude mice was investigated in vivo. Our results showed that ligustrazine could significantly inhibit the proliferation, migration, and invasion of A498 both in vivo and vitro. Western blot analysis showed that the expressions of EMT-related, N-cadherin, snail, and slug proteins were significantly decreased in A498 in the ligustrazine treatment group. This study indicated that ligustrazine could significantly inhibit the malignant biological behaviors of RCC cell lines, possibly by inhibiting the EMT process.
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| first_indexed | 2024-04-09T17:28:14Z |
| format | Article |
| id | doaj.art-785f9dd9f2d54f259fe154f6089e74e7 |
| institution | Directory Open Access Journal |
| issn | 2203-5826 |
| language | English |
| last_indexed | 2024-04-09T17:28:14Z |
| publishDate | 2023-01-01 |
| publisher | Codon Publications |
| record_format | Article |
| series | Journal of Kidney Cancer and VHL |
| spelling | doaj.art-785f9dd9f2d54f259fe154f6089e74e72023-04-18T09:18:47ZengCodon PublicationsJournal of Kidney Cancer and VHL2203-58262023-01-0110110.15586/jkcvhl.v10i1.232Ligustrazine Inhibits the Migration and Invasion of Renal Cell CarcinomaXufeng Zhang0Junfu Wang1Yanhua Cao2Kalin Li3Chao Sun4Wen Jiang5Qian Xin6Jue Wang7Tonggang Qi8Shuangde Liu9Yun Luan10Department of Renal Transplantation, The Second Hospital of Shandong University, Jinan, PR China.College of Pharmacy, Shandong First Medical University & Shandong Academy of Medical Sciences, Taian, PR China.Department of Pharmacy, The Second Hospital of Shandong University, Jinan, PR China.Institute of Medical Sciences, The Second Hospital of Shandong University, Jinan, PR China.Institute of Medical Sciences, The Second Hospital of Shandong University, Jinan, PR China.Institute of Medical Sciences, The Second Hospital of Shandong University, Jinan, PR China.Institute of Medical Sciences, The Second Hospital of Shandong University, Jinan, PR China.Institute of Medical Sciences, The Second Hospital of Shandong University, Jinan, PR China.Institute of Medical Sciences, The Second Hospital of Shandong University, Jinan, PR China.Department of Renal Transplantation, The Second Hospital of Shandong University, Jinan, PR China.Institute of Medical Sciences, The Second Hospital of Shandong University, Jinan, PR China. Ligustrazine is a Chinese herb (Chuanxiong) approved for use as a medical drug in China. Recent evidence suggests that ligustrazine has promising antitumor properties. Our preliminary results showed that ligustrazine could inhibit the growth of human renal cell carcinoma (RCC) cell lines. However, the complicated molecular mechanism has not been fully revealed. Therefore, the purpose of this study to investigate the mechanism of ligustrazine resistance in human RCC cells. Cell proliferation, migration, invasion, and colony-formation ability of RCC cells A498 were detected by MTT assay, clonal formation rates, and transwell chamber assay in vitro. The expression of epithelial–mesenchymal transition (EMT)–related proteins were analyzed using western blot test. The effect of ligustrazine on the growth of A498 cells in nude mice was investigated in vivo. Our results showed that ligustrazine could significantly inhibit the proliferation, migration, and invasion of A498 both in vivo and vitro. Western blot analysis showed that the expressions of EMT-related, N-cadherin, snail, and slug proteins were significantly decreased in A498 in the ligustrazine treatment group. This study indicated that ligustrazine could significantly inhibit the malignant biological behaviors of RCC cell lines, possibly by inhibiting the EMT process. https://mail.jkcvhl.com/~jkcvhlco/index.php/jkcvhl/article/view/232clear cell carcinomaepithelial–mesenchymal transitionligustrazinemigrationproliferation |
| spellingShingle | Xufeng Zhang Junfu Wang Yanhua Cao Kalin Li Chao Sun Wen Jiang Qian Xin Jue Wang Tonggang Qi Shuangde Liu Yun Luan Ligustrazine Inhibits the Migration and Invasion of Renal Cell Carcinoma Journal of Kidney Cancer and VHL clear cell carcinoma epithelial–mesenchymal transition ligustrazine migration proliferation |
| title | Ligustrazine Inhibits the Migration and Invasion of Renal Cell Carcinoma |
| title_full | Ligustrazine Inhibits the Migration and Invasion of Renal Cell Carcinoma |
| title_fullStr | Ligustrazine Inhibits the Migration and Invasion of Renal Cell Carcinoma |
| title_full_unstemmed | Ligustrazine Inhibits the Migration and Invasion of Renal Cell Carcinoma |
| title_short | Ligustrazine Inhibits the Migration and Invasion of Renal Cell Carcinoma |
| title_sort | ligustrazine inhibits the migration and invasion of renal cell carcinoma |
| topic | clear cell carcinoma epithelial–mesenchymal transition ligustrazine migration proliferation |
| url | https://mail.jkcvhl.com/~jkcvhlco/index.php/jkcvhl/article/view/232 |
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