Antibiotic Susceptibility Profile and Tetracycline Resistance Genes Detection in <i>Salmonella</i> spp. Strains Isolated from Animals and Food
<i>Salmonella</i> spp. is among the leading causes of foodborne infections in humans and a large number of animals. <i>Salmonella</i> spp. is a pathogen involved in the dissemination of antimicrobial resistance because it can accumulate antibiotic resistance genes (ARGs). In...
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| Format: | Article |
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MDPI AG
2021-07-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/10/7/809 |
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| author | Valeria Gargano Sonia Sciortino Delia Gambino Antonella Costa Vincenzo Agozzino Stefano Reale Rosa Alduina Domenico Vicari |
| author_facet | Valeria Gargano Sonia Sciortino Delia Gambino Antonella Costa Vincenzo Agozzino Stefano Reale Rosa Alduina Domenico Vicari |
| author_sort | Valeria Gargano |
| collection | DOAJ |
| description | <i>Salmonella</i> spp. is among the leading causes of foodborne infections in humans and a large number of animals. <i>Salmonella</i> spp. is a pathogen involved in the dissemination of antimicrobial resistance because it can accumulate antibiotic resistance genes (ARGs). In this study, the antibiotic resistance profile to 15 antibiotics, belonging to six different classes, of 60 strains of <i>Salmonella</i> spp. collected from pets, farm animals, wildlife, and food in Sicily (Italy) was investigated by the Kirby-Bauer method. Given that almost 33.3% of the <i>Salmonella</i> spp. strains were resistant to tetracycline, Real-Time PCR analysis was applied on all the 60 strains to detect the presence of eight selected <i>tet</i> resistance genes. Besides, the presence of the <i>int1</i> gene, related to the horizontal gene transfer among bacteria, was also investigated in all the strains by Real-Time PCR analysis. Our data showed that 56% of the isolated strains harbored one or more <i>tet</i> resistance genes and that these strains were most frequently isolated from animals living in close contact with humans. Concerning <i>int1</i>, 17 strains (28.3%) harbored this genetic element and eight of these simultaneously contained <i>tet</i> genes. The results of this study highlight the importance of using a molecular approach to detect resistance genetic determinants, whose spread can increase the diffusion of multidrug-resistant strains. Besides, the study of zoonotic bacteria such as <i>Salmonella</i> spp. which significantly contribute to ARGs dissemination should always follow a One Health approach that considers the health of humans, animals, and the environment to be closely related. |
| first_indexed | 2024-03-10T09:48:00Z |
| format | Article |
| id | doaj.art-78611da5f1b447f496aaa970407df64a |
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| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T09:48:00Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-78611da5f1b447f496aaa970407df64a2023-11-22T03:03:29ZengMDPI AGAntibiotics2079-63822021-07-0110780910.3390/antibiotics10070809Antibiotic Susceptibility Profile and Tetracycline Resistance Genes Detection in <i>Salmonella</i> spp. Strains Isolated from Animals and FoodValeria Gargano0Sonia Sciortino1Delia Gambino2Antonella Costa3Vincenzo Agozzino4Stefano Reale5Rosa Alduina6Domenico Vicari7Istituto Zooprofilattico Sperimentale della Sicilia, 90129 Palermo, ItalyIstituto Zooprofilattico Sperimentale della Sicilia, 90129 Palermo, ItalyIstituto Zooprofilattico Sperimentale della Sicilia, 90129 Palermo, ItalyIstituto Zooprofilattico Sperimentale della Sicilia, 90129 Palermo, ItalyIstituto Zooprofilattico Sperimentale della Sicilia, 90129 Palermo, ItalyIstituto Zooprofilattico Sperimentale della Sicilia, 90129 Palermo, ItalyDepartment of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, 90028 Palermo, ItalyIstituto Zooprofilattico Sperimentale della Sicilia, 90129 Palermo, Italy<i>Salmonella</i> spp. is among the leading causes of foodborne infections in humans and a large number of animals. <i>Salmonella</i> spp. is a pathogen involved in the dissemination of antimicrobial resistance because it can accumulate antibiotic resistance genes (ARGs). In this study, the antibiotic resistance profile to 15 antibiotics, belonging to six different classes, of 60 strains of <i>Salmonella</i> spp. collected from pets, farm animals, wildlife, and food in Sicily (Italy) was investigated by the Kirby-Bauer method. Given that almost 33.3% of the <i>Salmonella</i> spp. strains were resistant to tetracycline, Real-Time PCR analysis was applied on all the 60 strains to detect the presence of eight selected <i>tet</i> resistance genes. Besides, the presence of the <i>int1</i> gene, related to the horizontal gene transfer among bacteria, was also investigated in all the strains by Real-Time PCR analysis. Our data showed that 56% of the isolated strains harbored one or more <i>tet</i> resistance genes and that these strains were most frequently isolated from animals living in close contact with humans. Concerning <i>int1</i>, 17 strains (28.3%) harbored this genetic element and eight of these simultaneously contained <i>tet</i> genes. The results of this study highlight the importance of using a molecular approach to detect resistance genetic determinants, whose spread can increase the diffusion of multidrug-resistant strains. Besides, the study of zoonotic bacteria such as <i>Salmonella</i> spp. which significantly contribute to ARGs dissemination should always follow a One Health approach that considers the health of humans, animals, and the environment to be closely related.https://www.mdpi.com/2079-6382/10/7/809<i>Salmonella</i>antibiotic resistanceARGs<i>tet</i> genesanimalsfood |
| spellingShingle | Valeria Gargano Sonia Sciortino Delia Gambino Antonella Costa Vincenzo Agozzino Stefano Reale Rosa Alduina Domenico Vicari Antibiotic Susceptibility Profile and Tetracycline Resistance Genes Detection in <i>Salmonella</i> spp. Strains Isolated from Animals and Food Antibiotics <i>Salmonella</i> antibiotic resistance ARGs <i>tet</i> genes animals food |
| title | Antibiotic Susceptibility Profile and Tetracycline Resistance Genes Detection in <i>Salmonella</i> spp. Strains Isolated from Animals and Food |
| title_full | Antibiotic Susceptibility Profile and Tetracycline Resistance Genes Detection in <i>Salmonella</i> spp. Strains Isolated from Animals and Food |
| title_fullStr | Antibiotic Susceptibility Profile and Tetracycline Resistance Genes Detection in <i>Salmonella</i> spp. Strains Isolated from Animals and Food |
| title_full_unstemmed | Antibiotic Susceptibility Profile and Tetracycline Resistance Genes Detection in <i>Salmonella</i> spp. Strains Isolated from Animals and Food |
| title_short | Antibiotic Susceptibility Profile and Tetracycline Resistance Genes Detection in <i>Salmonella</i> spp. Strains Isolated from Animals and Food |
| title_sort | antibiotic susceptibility profile and tetracycline resistance genes detection in i salmonella i spp strains isolated from animals and food |
| topic | <i>Salmonella</i> antibiotic resistance ARGs <i>tet</i> genes animals food |
| url | https://www.mdpi.com/2079-6382/10/7/809 |
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