In vitro anthelmintic activity of Chenopodium album and in-silico prediction of mechanistic role on Eisenia foetida
Helminths born diseases are related to pitiable management practices and improper control strategies. The medicinal plants contain various phytoconstituents that are liable for their anthelmintic activity. The aerial parts of the Chenopodium album were successively extracted with microwaves assisted...
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Elsevier
2021-01-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844021000220 |
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| author | Neeraj Choudhary Gopal L. Khatik Sunanda Choudhary Gurpal Singh Ashish Suttee |
| author_facet | Neeraj Choudhary Gopal L. Khatik Sunanda Choudhary Gurpal Singh Ashish Suttee |
| author_sort | Neeraj Choudhary |
| collection | DOAJ |
| description | Helminths born diseases are related to pitiable management practices and improper control strategies. The medicinal plants contain various phytoconstituents that are liable for their anthelmintic activity. The aerial parts of the Chenopodium album were successively extracted with microwaves assisted extraction using petroleum ether, ethyl acetate, methanol, hydroalcoholic and aqueous solvents to get respective extracts (CAPE, CAEE, CAME, CAHE, and CAAE). All the extracts were analyzed for preliminary phytochemical screening for the identification of phytoconstituents. The anthelmintic activity was analyzed on Indian adult earthworms Eisenia foetida using piperazine citrate (PCT) as a standard drug. All the extracts (apart from CAAE) lead to paralysis and fatality of the earthworms. CAEE extract exhibits highly significant anthelmintic activity at a 10 mg/ml concentration by causing paralysis and fatality of earthworms and was more potent than PCT suspension. At a concentration of 10 mg/ml, paralysis and death time for CAEE was recorded as (10.08 ± 1.11) and (65.28 ± 2.09) respectively, while for standard piperazine citrate, it was recorded as (22.96 ± 1.12) and (65.09 ± 1.23). The CAEE exhibits two major compounds by LC-MS, i.e., NG and DG, that are mainly accountable for the Chenopodium album anthelmintic activity. The plant possesses GABA-mimetic action and thereby leads to flaccid, reversible paralysis of the body wall muscle. |
| first_indexed | 2024-12-14T06:51:37Z |
| format | Article |
| id | doaj.art-7863bc39328d4857b5edaabb71ee478a |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-12-14T06:51:37Z |
| publishDate | 2021-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-7863bc39328d4857b5edaabb71ee478a2022-12-21T23:12:53ZengElsevierHeliyon2405-84402021-01-0171e05917In vitro anthelmintic activity of Chenopodium album and in-silico prediction of mechanistic role on Eisenia foetidaNeeraj Choudhary0Gopal L. Khatik1Sunanda Choudhary2Gurpal Singh3Ashish Suttee4School of Pharmaceutical Sciences, Lovely Professional University, Phagwara, Punjab, India; Faculty of Pharmaceutical Sciences, PCTE Group of Institutes, Ludhiana, Punjab, IndiaSchool of Pharmaceutical Sciences, Lovely Professional University, Phagwara, Punjab, IndiaSchool of Pharmaceutical and Health Care Sciences, CT University, Sidhwan Khurd, Ludhiana, Punjab, IndiaUIPS, Punjab University, Chandigarh, IndiaSchool of Pharmaceutical Sciences, Lovely Professional University, Phagwara, Punjab, India; Corresponding author.Helminths born diseases are related to pitiable management practices and improper control strategies. The medicinal plants contain various phytoconstituents that are liable for their anthelmintic activity. The aerial parts of the Chenopodium album were successively extracted with microwaves assisted extraction using petroleum ether, ethyl acetate, methanol, hydroalcoholic and aqueous solvents to get respective extracts (CAPE, CAEE, CAME, CAHE, and CAAE). All the extracts were analyzed for preliminary phytochemical screening for the identification of phytoconstituents. The anthelmintic activity was analyzed on Indian adult earthworms Eisenia foetida using piperazine citrate (PCT) as a standard drug. All the extracts (apart from CAAE) lead to paralysis and fatality of the earthworms. CAEE extract exhibits highly significant anthelmintic activity at a 10 mg/ml concentration by causing paralysis and fatality of earthworms and was more potent than PCT suspension. At a concentration of 10 mg/ml, paralysis and death time for CAEE was recorded as (10.08 ± 1.11) and (65.28 ± 2.09) respectively, while for standard piperazine citrate, it was recorded as (22.96 ± 1.12) and (65.09 ± 1.23). The CAEE exhibits two major compounds by LC-MS, i.e., NG and DG, that are mainly accountable for the Chenopodium album anthelmintic activity. The plant possesses GABA-mimetic action and thereby leads to flaccid, reversible paralysis of the body wall muscle.http://www.sciencedirect.com/science/article/pii/S2405844021000220Chenopodium albumEisenia foetidaPiperazine citrateAnthelmintic activityLC-MS |
| spellingShingle | Neeraj Choudhary Gopal L. Khatik Sunanda Choudhary Gurpal Singh Ashish Suttee In vitro anthelmintic activity of Chenopodium album and in-silico prediction of mechanistic role on Eisenia foetida Heliyon Chenopodium album Eisenia foetida Piperazine citrate Anthelmintic activity LC-MS |
| title | In vitro anthelmintic activity of Chenopodium album and in-silico prediction of mechanistic role on Eisenia foetida |
| title_full | In vitro anthelmintic activity of Chenopodium album and in-silico prediction of mechanistic role on Eisenia foetida |
| title_fullStr | In vitro anthelmintic activity of Chenopodium album and in-silico prediction of mechanistic role on Eisenia foetida |
| title_full_unstemmed | In vitro anthelmintic activity of Chenopodium album and in-silico prediction of mechanistic role on Eisenia foetida |
| title_short | In vitro anthelmintic activity of Chenopodium album and in-silico prediction of mechanistic role on Eisenia foetida |
| title_sort | in vitro anthelmintic activity of chenopodium album and in silico prediction of mechanistic role on eisenia foetida |
| topic | Chenopodium album Eisenia foetida Piperazine citrate Anthelmintic activity LC-MS |
| url | http://www.sciencedirect.com/science/article/pii/S2405844021000220 |
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