Identification of Promiscuous African Swine Fever Virus T-Cell Determinants Using a Multiple Technical Approach
The development of subunit vaccines against African swine fever (ASF) is mainly hindered by the lack of knowledge regarding the specific ASF virus (ASFV) antigens involved in protection. As a good example, the identity of ASFV-specific CD8<sup>+</sup> T-cell determinants remains largely...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-01-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/9/1/29 |
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| author | Laia Bosch-Camós Elisabet López María Jesús Navas Sonia Pina-Pedrero Francesc Accensi Florencia Correa-Fiz Chankyu Park Montserrat Carrascal Javier Domínguez Maria Luisa Salas Veljko Nikolin Javier Collado Fernando Rodríguez |
| author_facet | Laia Bosch-Camós Elisabet López María Jesús Navas Sonia Pina-Pedrero Francesc Accensi Florencia Correa-Fiz Chankyu Park Montserrat Carrascal Javier Domínguez Maria Luisa Salas Veljko Nikolin Javier Collado Fernando Rodríguez |
| author_sort | Laia Bosch-Camós |
| collection | DOAJ |
| description | The development of subunit vaccines against African swine fever (ASF) is mainly hindered by the lack of knowledge regarding the specific ASF virus (ASFV) antigens involved in protection. As a good example, the identity of ASFV-specific CD8<sup>+</sup> T-cell determinants remains largely unknown, despite their protective role being established a long time ago. Aiming to identify them, we implemented the IFNγ ELISpot as readout assay, using as effector cells peripheral blood mononuclear cells (PBMCs) from pigs surviving experimental challenge with Georgia2007/1. As stimuli for the ELISpot, ASFV-specific peptides or full-length proteins identified by three complementary strategies were used. In silico prediction of specific CD8<sup>+</sup> T-cell epitopes allowed identifying a 19-mer peptide from MGF100-1L, as frequently recognized by surviving pigs. Complementarily, the repertoire of SLA I-bound peptides identified in ASFV-infected porcine alveolar macrophages (PAMs), allowed the characterization of five additional SLA I-restricted ASFV-specific epitopes. Finally, in vitro stimulation studies using fibroblasts transfected with plasmids encoding full-length ASFV proteins, led to the identification of MGF505-7R, A238L and MGF100-1L as promiscuously recognized antigens. Interestingly, each one of these proteins contain individual peptides recognized by surviving pigs. Identification of the same ASFV determinants by means of such different approaches reinforce the results presented here. |
| first_indexed | 2024-03-09T05:46:11Z |
| format | Article |
| id | doaj.art-7864c3b4ad6047f2b5a183828f6ede1d |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-09T05:46:11Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-7864c3b4ad6047f2b5a183828f6ede1d2023-12-03T12:20:34ZengMDPI AGVaccines2076-393X2021-01-01912910.3390/vaccines9010029Identification of Promiscuous African Swine Fever Virus T-Cell Determinants Using a Multiple Technical ApproachLaia Bosch-Camós0Elisabet López1María Jesús Navas2Sonia Pina-Pedrero3Francesc Accensi4Florencia Correa-Fiz5Chankyu Park6Montserrat Carrascal7Javier Domínguez8Maria Luisa Salas9Veljko Nikolin10Javier Collado11Fernando Rodríguez12IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA), Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra, SpainIRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA), Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra, SpainIRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA), Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra, SpainIRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA), Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra, SpainOIE Collaborating Centre for the Research and Control of Emerging and Re-Emerging Swine Diseases in Europe (IRTA-CReSA), 08193 Bellaterra, SpainIRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA), Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra, SpainDepartment of Stem Cells and Regenerative Biology, Konkuk University, Seoul 05029, KoreaInstituto de Investigaciones Biomédicas de Barcelona-Unidad de Espectrometría de Masas Biológica y Proteómica, Consejo Superior de Investigaciones Científicas (CSIC), 08193 Bellaterra, SpainDepartamento de Biotecnología, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), 28049 Madrid, SpainCentro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas and Universidad Autònoma de Madrid, 28049 Madrid, SpainBoehringer Ingelheim Veterinary Research Center (BIVRC) GmbH & Co. KG, 30559 Hannover, GermanyDepartament de Biologia Cel·lular, Fisiologia i Immunologia, Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra, SpainIRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA), Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra, SpainThe development of subunit vaccines against African swine fever (ASF) is mainly hindered by the lack of knowledge regarding the specific ASF virus (ASFV) antigens involved in protection. As a good example, the identity of ASFV-specific CD8<sup>+</sup> T-cell determinants remains largely unknown, despite their protective role being established a long time ago. Aiming to identify them, we implemented the IFNγ ELISpot as readout assay, using as effector cells peripheral blood mononuclear cells (PBMCs) from pigs surviving experimental challenge with Georgia2007/1. As stimuli for the ELISpot, ASFV-specific peptides or full-length proteins identified by three complementary strategies were used. In silico prediction of specific CD8<sup>+</sup> T-cell epitopes allowed identifying a 19-mer peptide from MGF100-1L, as frequently recognized by surviving pigs. Complementarily, the repertoire of SLA I-bound peptides identified in ASFV-infected porcine alveolar macrophages (PAMs), allowed the characterization of five additional SLA I-restricted ASFV-specific epitopes. Finally, in vitro stimulation studies using fibroblasts transfected with plasmids encoding full-length ASFV proteins, led to the identification of MGF505-7R, A238L and MGF100-1L as promiscuously recognized antigens. Interestingly, each one of these proteins contain individual peptides recognized by surviving pigs. Identification of the same ASFV determinants by means of such different approaches reinforce the results presented here.https://www.mdpi.com/2076-393X/9/1/29ASFVCD8<sup>+</sup> T-cellsantigen presentationIFNγ ELISpotepitope predictionsimmunopeptidomics |
| spellingShingle | Laia Bosch-Camós Elisabet López María Jesús Navas Sonia Pina-Pedrero Francesc Accensi Florencia Correa-Fiz Chankyu Park Montserrat Carrascal Javier Domínguez Maria Luisa Salas Veljko Nikolin Javier Collado Fernando Rodríguez Identification of Promiscuous African Swine Fever Virus T-Cell Determinants Using a Multiple Technical Approach Vaccines ASFV CD8<sup>+</sup> T-cells antigen presentation IFNγ ELISpot epitope predictions immunopeptidomics |
| title | Identification of Promiscuous African Swine Fever Virus T-Cell Determinants Using a Multiple Technical Approach |
| title_full | Identification of Promiscuous African Swine Fever Virus T-Cell Determinants Using a Multiple Technical Approach |
| title_fullStr | Identification of Promiscuous African Swine Fever Virus T-Cell Determinants Using a Multiple Technical Approach |
| title_full_unstemmed | Identification of Promiscuous African Swine Fever Virus T-Cell Determinants Using a Multiple Technical Approach |
| title_short | Identification of Promiscuous African Swine Fever Virus T-Cell Determinants Using a Multiple Technical Approach |
| title_sort | identification of promiscuous african swine fever virus t cell determinants using a multiple technical approach |
| topic | ASFV CD8<sup>+</sup> T-cells antigen presentation IFNγ ELISpot epitope predictions immunopeptidomics |
| url | https://www.mdpi.com/2076-393X/9/1/29 |
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