An Interdisciplinary Approach to Study the Performance of Second-generation Genetically Modified Crops in Field Trials: A Case Study With Soybean and Wheat Carrying the Sunflower HaHB4 Transcription Factor
Research, production, and use of genetically modified (GM) crops have split the world between supporters and opponents. Up to now, this technology has been limited to the control of weeds and pests, whereas the second generation of GM crops is expected to assist farmers in abiotic stress tolerance o...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-03-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2020.00178/full |
| _version_ | 1818675317303148544 |
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| author | Fernanda Gabriela González Nicolás Rigalli Patricia Vivian Miranda Patricia Vivian Miranda Martín Romagnoli Karina Fabiana Ribichich Federico Trucco Margarita Portapila María Elena Otegui Raquel Lía Chan |
| author_facet | Fernanda Gabriela González Nicolás Rigalli Patricia Vivian Miranda Patricia Vivian Miranda Martín Romagnoli Karina Fabiana Ribichich Federico Trucco Margarita Portapila María Elena Otegui Raquel Lía Chan |
| author_sort | Fernanda Gabriela González |
| collection | DOAJ |
| description | Research, production, and use of genetically modified (GM) crops have split the world between supporters and opponents. Up to now, this technology has been limited to the control of weeds and pests, whereas the second generation of GM crops is expected to assist farmers in abiotic stress tolerance or improved nutritional features. Aiming to analyze this subject holistically, in this presentation we address an advanced technology for drought-tolerant GM crops, upscaling from molecular details obtained in the laboratory to an extensive network of field trials as well as the impact of the introduction of this innovation into the market. Sunflower has divergent transcription factors, which could be key actors in the drought response orchestrating several signal transduction pathways, generating an improved performance to deal with water deficit. One of such factors, HaHB4, belongs to the homeodomain-leucine zipper family and was first introduced in Arabidopsis. Transformed plants had improved tolerance to water deficits, through the inhibition of ethylene sensitivity and not by stomata closure. Wheat and soybean plants expressing the HaHB4 gene were obtained and cropped across a wide range of growing conditions exhibiting enhanced adaptation to drought-prone environments, the most important constraint affecting crop yield worldwide. The performance of wheat and soybean, however, differed slightly across mentioned environments; whereas the improved behavior of GM wheat respect to controls was less dependent on the temperature regime (cool or warm), differences between GM and wild-type soybeans were remarkably larger in warmer compared to cooler conditions. In both species, these GM crops are good candidates to become market products in the near future. In anticipation of consumers’ and other stakeholders’ interest, spectral analyses of field crops have been conducted to differentiate these GM crops from wild type and commercial cultivars. In this paper, the potential impact of the release of such market products is discussed, considering the perspectives of different stakeholders. |
| first_indexed | 2024-12-17T08:25:39Z |
| format | Article |
| id | doaj.art-7865914e6a1d4928b8acb4eff19b0966 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-17T08:25:39Z |
| publishDate | 2020-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-7865914e6a1d4928b8acb4eff19b09662022-12-21T21:56:47ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2020-03-011110.3389/fpls.2020.00178506461An Interdisciplinary Approach to Study the Performance of Second-generation Genetically Modified Crops in Field Trials: A Case Study With Soybean and Wheat Carrying the Sunflower HaHB4 Transcription FactorFernanda Gabriela González0Nicolás Rigalli1Patricia Vivian Miranda2Patricia Vivian Miranda3Martín Romagnoli4Karina Fabiana Ribichich5Federico Trucco6Margarita Portapila7María Elena Otegui8Raquel Lía Chan9Estación Experimental Pergamino, INTA, CITNOBA, CONICET-UNNOBA, Pergamino, ArgentinaCIFASIS, Universidad Nacional de Rosario—CONICET, Rosario, ArgentinaInstituto de Agrobiotecnología Rosario (INDEAR)/BIOCERES, Rosario, ArgentinaCONICET, Buenos Aires, ArgentinaCIFASIS, Universidad Nacional de Rosario—CONICET, Rosario, ArgentinaInstituto de Agrobiotecnología del Litoral, Universidad Nacional del Litoral—CONICET, Facultad de Bioquímica y Ciencias Biológicas, Santa Fe, ArgentinaInstituto de Agrobiotecnología Rosario (INDEAR)/BIOCERES, Rosario, ArgentinaCIFASIS, Universidad Nacional de Rosario—CONICET, Rosario, ArgentinaCONICET-INTA-FAUBA, Estación Experimental Pergamino, Facultad de Agronomía Universidad de Buenos Aires, Pergamino, ArgentinaInstituto de Agrobiotecnología del Litoral, Universidad Nacional del Litoral—CONICET, Facultad de Bioquímica y Ciencias Biológicas, Santa Fe, ArgentinaResearch, production, and use of genetically modified (GM) crops have split the world between supporters and opponents. Up to now, this technology has been limited to the control of weeds and pests, whereas the second generation of GM crops is expected to assist farmers in abiotic stress tolerance or improved nutritional features. Aiming to analyze this subject holistically, in this presentation we address an advanced technology for drought-tolerant GM crops, upscaling from molecular details obtained in the laboratory to an extensive network of field trials as well as the impact of the introduction of this innovation into the market. Sunflower has divergent transcription factors, which could be key actors in the drought response orchestrating several signal transduction pathways, generating an improved performance to deal with water deficit. One of such factors, HaHB4, belongs to the homeodomain-leucine zipper family and was first introduced in Arabidopsis. Transformed plants had improved tolerance to water deficits, through the inhibition of ethylene sensitivity and not by stomata closure. Wheat and soybean plants expressing the HaHB4 gene were obtained and cropped across a wide range of growing conditions exhibiting enhanced adaptation to drought-prone environments, the most important constraint affecting crop yield worldwide. The performance of wheat and soybean, however, differed slightly across mentioned environments; whereas the improved behavior of GM wheat respect to controls was less dependent on the temperature regime (cool or warm), differences between GM and wild-type soybeans were remarkably larger in warmer compared to cooler conditions. In both species, these GM crops are good candidates to become market products in the near future. In anticipation of consumers’ and other stakeholders’ interest, spectral analyses of field crops have been conducted to differentiate these GM crops from wild type and commercial cultivars. In this paper, the potential impact of the release of such market products is discussed, considering the perspectives of different stakeholders.https://www.frontiersin.org/article/10.3389/fpls.2020.00178/fulltransgenic wheattransgenic soybeanHaHB4sunflower transcription factordrought tolerancegrain yield determination |
| spellingShingle | Fernanda Gabriela González Nicolás Rigalli Patricia Vivian Miranda Patricia Vivian Miranda Martín Romagnoli Karina Fabiana Ribichich Federico Trucco Margarita Portapila María Elena Otegui Raquel Lía Chan An Interdisciplinary Approach to Study the Performance of Second-generation Genetically Modified Crops in Field Trials: A Case Study With Soybean and Wheat Carrying the Sunflower HaHB4 Transcription Factor Frontiers in Plant Science transgenic wheat transgenic soybean HaHB4 sunflower transcription factor drought tolerance grain yield determination |
| title | An Interdisciplinary Approach to Study the Performance of Second-generation Genetically Modified Crops in Field Trials: A Case Study With Soybean and Wheat Carrying the Sunflower HaHB4 Transcription Factor |
| title_full | An Interdisciplinary Approach to Study the Performance of Second-generation Genetically Modified Crops in Field Trials: A Case Study With Soybean and Wheat Carrying the Sunflower HaHB4 Transcription Factor |
| title_fullStr | An Interdisciplinary Approach to Study the Performance of Second-generation Genetically Modified Crops in Field Trials: A Case Study With Soybean and Wheat Carrying the Sunflower HaHB4 Transcription Factor |
| title_full_unstemmed | An Interdisciplinary Approach to Study the Performance of Second-generation Genetically Modified Crops in Field Trials: A Case Study With Soybean and Wheat Carrying the Sunflower HaHB4 Transcription Factor |
| title_short | An Interdisciplinary Approach to Study the Performance of Second-generation Genetically Modified Crops in Field Trials: A Case Study With Soybean and Wheat Carrying the Sunflower HaHB4 Transcription Factor |
| title_sort | interdisciplinary approach to study the performance of second generation genetically modified crops in field trials a case study with soybean and wheat carrying the sunflower hahb4 transcription factor |
| topic | transgenic wheat transgenic soybean HaHB4 sunflower transcription factor drought tolerance grain yield determination |
| url | https://www.frontiersin.org/article/10.3389/fpls.2020.00178/full |
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