Analgesic and Anxiolytic Effects of Gastrodin and Its Influences on Ferroptosis and Jejunal Microbiota in Complete Freund’s Adjuvant-Injected Mice
This study investigated the effects of gastrodin (GAS) on analgesic, anxiolytic, ferroptosis, and jejunal microbiota in chronic inflammatory pain mice. The chronic inflammatory pain model of C57BL/6J mice was established by hindpaw injection of complete Freund’s adjuvant (CFA). After GAS treatment,...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-04-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.841662/full |
| _version_ | 1818028598024470528 |
|---|---|
| author | Xin Chen Xin Chen Jinyue Wang Zhixian He Xin Liu Huawei Liu Xing Wang |
| author_facet | Xin Chen Xin Chen Jinyue Wang Zhixian He Xin Liu Huawei Liu Xing Wang |
| author_sort | Xin Chen |
| collection | DOAJ |
| description | This study investigated the effects of gastrodin (GAS) on analgesic, anxiolytic, ferroptosis, and jejunal microbiota in chronic inflammatory pain mice. The chronic inflammatory pain model of C57BL/6J mice was established by hindpaw injection of complete Freund’s adjuvant (CFA). After GAS treatment, thermal hyperalgesia test, mechanical allodynia test, elevated plus-maze (EPMT), and open-field test (OFT) were performed to assess the behavioral changes of pain and anxiety. mRNAs of FTHI, GPX4, HO-1, and PTGS2 and jejunal microbiota were measured by qPCR. In CFA-injected C57BL/6 mice, we found that the mechanical and thermal pain threshold were increased with treatment of GAS. In EPMT, the number of entries in open arms and retention times of open arms were increased by GAS. In the OFT, the time spent in the central area was also increased. Furthermore, GAS enhanced mRNA expressions of FTHI, GPX4, and HO-1 but decreased the expression of PTGS2 in a dose-dependent manner. GAS is effective in the treatment of mice chronic inflammatory pain and anxiety-like behaviors. It may be exhibits potential neuroprotective effects through inhibition of ferroptosis independently of the intestinal microbiota. |
| first_indexed | 2024-12-10T05:06:20Z |
| format | Article |
| id | doaj.art-7867cf62a26b4029ae6cd8dab38de771 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-10T05:06:20Z |
| publishDate | 2022-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-7867cf62a26b4029ae6cd8dab38de7712022-12-22T02:01:13ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-04-011310.3389/fmicb.2022.841662841662Analgesic and Anxiolytic Effects of Gastrodin and Its Influences on Ferroptosis and Jejunal Microbiota in Complete Freund’s Adjuvant-Injected MiceXin Chen0Xin Chen1Jinyue Wang2Zhixian He3Xin Liu4Huawei Liu5Xing Wang6College of Medicine, Southwest Jiaotong University, Chengdu, ChinaDepartment of Laboratory Medicine, The Affiliated Hospital of Southwest Jiaotong University, The Third People’s Hospital of Chengdu, Chengdu, ChinaCollege of Medicine, Southwest Jiaotong University, Chengdu, ChinaCollege of Medicine, Southwest Jiaotong University, Chengdu, ChinaCollege of Medicine, Southwest Jiaotong University, Chengdu, ChinaDepartment of Laboratory Medicine, The Affiliated Hospital of Southwest Jiaotong University, The Third People’s Hospital of Chengdu, Chengdu, ChinaCollege of Medicine, Southwest Jiaotong University, Chengdu, ChinaThis study investigated the effects of gastrodin (GAS) on analgesic, anxiolytic, ferroptosis, and jejunal microbiota in chronic inflammatory pain mice. The chronic inflammatory pain model of C57BL/6J mice was established by hindpaw injection of complete Freund’s adjuvant (CFA). After GAS treatment, thermal hyperalgesia test, mechanical allodynia test, elevated plus-maze (EPMT), and open-field test (OFT) were performed to assess the behavioral changes of pain and anxiety. mRNAs of FTHI, GPX4, HO-1, and PTGS2 and jejunal microbiota were measured by qPCR. In CFA-injected C57BL/6 mice, we found that the mechanical and thermal pain threshold were increased with treatment of GAS. In EPMT, the number of entries in open arms and retention times of open arms were increased by GAS. In the OFT, the time spent in the central area was also increased. Furthermore, GAS enhanced mRNA expressions of FTHI, GPX4, and HO-1 but decreased the expression of PTGS2 in a dose-dependent manner. GAS is effective in the treatment of mice chronic inflammatory pain and anxiety-like behaviors. It may be exhibits potential neuroprotective effects through inhibition of ferroptosis independently of the intestinal microbiota.https://www.frontiersin.org/articles/10.3389/fmicb.2022.841662/fullgastrodinanalgesicanxiolyticferroptosismicrobiota |
| spellingShingle | Xin Chen Xin Chen Jinyue Wang Zhixian He Xin Liu Huawei Liu Xing Wang Analgesic and Anxiolytic Effects of Gastrodin and Its Influences on Ferroptosis and Jejunal Microbiota in Complete Freund’s Adjuvant-Injected Mice Frontiers in Microbiology gastrodin analgesic anxiolytic ferroptosis microbiota |
| title | Analgesic and Anxiolytic Effects of Gastrodin and Its Influences on Ferroptosis and Jejunal Microbiota in Complete Freund’s Adjuvant-Injected Mice |
| title_full | Analgesic and Anxiolytic Effects of Gastrodin and Its Influences on Ferroptosis and Jejunal Microbiota in Complete Freund’s Adjuvant-Injected Mice |
| title_fullStr | Analgesic and Anxiolytic Effects of Gastrodin and Its Influences on Ferroptosis and Jejunal Microbiota in Complete Freund’s Adjuvant-Injected Mice |
| title_full_unstemmed | Analgesic and Anxiolytic Effects of Gastrodin and Its Influences on Ferroptosis and Jejunal Microbiota in Complete Freund’s Adjuvant-Injected Mice |
| title_short | Analgesic and Anxiolytic Effects of Gastrodin and Its Influences on Ferroptosis and Jejunal Microbiota in Complete Freund’s Adjuvant-Injected Mice |
| title_sort | analgesic and anxiolytic effects of gastrodin and its influences on ferroptosis and jejunal microbiota in complete freund s adjuvant injected mice |
| topic | gastrodin analgesic anxiolytic ferroptosis microbiota |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.841662/full |
| work_keys_str_mv | AT xinchen analgesicandanxiolyticeffectsofgastrodinanditsinfluencesonferroptosisandjejunalmicrobiotaincompletefreundsadjuvantinjectedmice AT xinchen analgesicandanxiolyticeffectsofgastrodinanditsinfluencesonferroptosisandjejunalmicrobiotaincompletefreundsadjuvantinjectedmice AT jinyuewang analgesicandanxiolyticeffectsofgastrodinanditsinfluencesonferroptosisandjejunalmicrobiotaincompletefreundsadjuvantinjectedmice AT zhixianhe analgesicandanxiolyticeffectsofgastrodinanditsinfluencesonferroptosisandjejunalmicrobiotaincompletefreundsadjuvantinjectedmice AT xinliu analgesicandanxiolyticeffectsofgastrodinanditsinfluencesonferroptosisandjejunalmicrobiotaincompletefreundsadjuvantinjectedmice AT huaweiliu analgesicandanxiolyticeffectsofgastrodinanditsinfluencesonferroptosisandjejunalmicrobiotaincompletefreundsadjuvantinjectedmice AT xingwang analgesicandanxiolyticeffectsofgastrodinanditsinfluencesonferroptosisandjejunalmicrobiotaincompletefreundsadjuvantinjectedmice |