Alterations of RNA Modification in Mouse Germ Cell-2 Spermatids Under Hypoxic Stress
Hypoxia is a known stress factor in mammals and has been shown to potentially impair male fertility, which manifests as spermatogenic dysfunction and decreased semen quality. Studies have shown that RNA modifications, the novel post-transcriptional regulators, are involved in spermatogenesis, and hy...
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Frontiers Media S.A.
2022-06-01
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| Series: | Frontiers in Molecular Biosciences |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmolb.2022.871737/full |
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| author | Tong He Tong He Huanping Guo Lin Xia Xipeng Shen Xipeng Shen Yun Huang Xiao Wu Xuelin Jiang Yinying Xu Yi Tan Yunfang Zhang Yunfang Zhang Dongmei Tan |
| author_facet | Tong He Tong He Huanping Guo Lin Xia Xipeng Shen Xipeng Shen Yun Huang Xiao Wu Xuelin Jiang Yinying Xu Yi Tan Yunfang Zhang Yunfang Zhang Dongmei Tan |
| author_sort | Tong He |
| collection | DOAJ |
| description | Hypoxia is a known stress factor in mammals and has been shown to potentially impair male fertility, which manifests as spermatogenic dysfunction and decreased semen quality. Studies have shown that RNA modifications, the novel post-transcriptional regulators, are involved in spermatogenesis, and hypoxia-induced alterations in RNA modification in testes and sperm cells may be associated with impaired spermatogenesis in mice. However, the molecular mechanisms via which RNA modifications influence spermatogenesis under hypoxic stress conditions are unclear. In this study, we generated a mouse Germ Cell-2 spermatid (GC-2spd) hypoxia model by culturing cells in a 1% O2 incubator for 48 h or treating them with CoCl2 for 24 h. The hypoxia treatment significantly inhibited proliferation and induced apoptosis in GC-2spd cells. The RNA modification signatures of total RNAs (2 types) and differentially sized RNA fragments (7 types of approximately 80 nt-sized tRNAs; 9 types of 17–50 nt-sized sncRNAs) were altered, and tRNA stability was partially affected. Moreover, the expression profiles of sncRNAs, such as microRNAs, tsRNAs, rsRNAs, and ysRNAs, were significantly regulated, and this might be related to the alterations in RNA modification and subsequent transcriptomic changes. We comprehensively analyzed alterations in RNA modification signatures in total RNAs, tRNAs (approximately 80 nt), and small RNAs (17–50 nt) as well as the expression profiles of sncRNAs and transcriptomes in hypoxia-treated GC-2spd cells; our data suggested that RNA modifications may be involved in cellular responses under hypoxic stress conditions and could provide a basis for a better understanding of the molecular mechanisms underlying male infertility. |
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| issn | 2296-889X |
| language | English |
| last_indexed | 2024-12-12T07:43:20Z |
| publishDate | 2022-06-01 |
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| series | Frontiers in Molecular Biosciences |
| spelling | doaj.art-786d76d1f72c4b3bae7ba08786df688b2022-12-22T00:32:43ZengFrontiers Media S.A.Frontiers in Molecular Biosciences2296-889X2022-06-01910.3389/fmolb.2022.871737871737Alterations of RNA Modification in Mouse Germ Cell-2 Spermatids Under Hypoxic StressTong He0Tong He1Huanping Guo2Lin Xia3Xipeng Shen4Xipeng Shen5Yun Huang6Xiao Wu7Xuelin Jiang8Yinying Xu9Yi Tan10Yunfang Zhang11Yunfang Zhang12Dongmei Tan13Laboratory Animal Center, Chongqing Medical University, Chongqing, ChinaMedical Center of Hematology, Xinqiao Hospital, Army Medical University, Chongqing, ChinaMedical Center of Hematology, Xinqiao Hospital, Army Medical University, Chongqing, ChinaMedical Center of Hematology, Xinqiao Hospital, Army Medical University, Chongqing, ChinaLaboratory Animal Center, Chongqing Medical University, Chongqing, ChinaMedical Center of Hematology, Xinqiao Hospital, Army Medical University, Chongqing, ChinaLaboratory Animal Center, Chongqing Medical University, Chongqing, ChinaMedical Center of Hematology, Xinqiao Hospital, Army Medical University, Chongqing, ChinaMedical Center of Hematology, Xinqiao Hospital, Army Medical University, Chongqing, ChinaMedical Center of Hematology, Xinqiao Hospital, Army Medical University, Chongqing, ChinaLaboratory Animal Center, Chongqing Medical University, Chongqing, ChinaMedical Center of Hematology, Xinqiao Hospital, Army Medical University, Chongqing, ChinaClinical and Translational Research Center of Shanghai First Maternity and Infant Hospital, Shanghai Key Laboratory of Signaling and Disease Research, School of Life Sciences and Technology, Tongji University, Shanghai, ChinaLaboratory Animal Center, Chongqing Medical University, Chongqing, ChinaHypoxia is a known stress factor in mammals and has been shown to potentially impair male fertility, which manifests as spermatogenic dysfunction and decreased semen quality. Studies have shown that RNA modifications, the novel post-transcriptional regulators, are involved in spermatogenesis, and hypoxia-induced alterations in RNA modification in testes and sperm cells may be associated with impaired spermatogenesis in mice. However, the molecular mechanisms via which RNA modifications influence spermatogenesis under hypoxic stress conditions are unclear. In this study, we generated a mouse Germ Cell-2 spermatid (GC-2spd) hypoxia model by culturing cells in a 1% O2 incubator for 48 h or treating them with CoCl2 for 24 h. The hypoxia treatment significantly inhibited proliferation and induced apoptosis in GC-2spd cells. The RNA modification signatures of total RNAs (2 types) and differentially sized RNA fragments (7 types of approximately 80 nt-sized tRNAs; 9 types of 17–50 nt-sized sncRNAs) were altered, and tRNA stability was partially affected. Moreover, the expression profiles of sncRNAs, such as microRNAs, tsRNAs, rsRNAs, and ysRNAs, were significantly regulated, and this might be related to the alterations in RNA modification and subsequent transcriptomic changes. We comprehensively analyzed alterations in RNA modification signatures in total RNAs, tRNAs (approximately 80 nt), and small RNAs (17–50 nt) as well as the expression profiles of sncRNAs and transcriptomes in hypoxia-treated GC-2spd cells; our data suggested that RNA modifications may be involved in cellular responses under hypoxic stress conditions and could provide a basis for a better understanding of the molecular mechanisms underlying male infertility.https://www.frontiersin.org/articles/10.3389/fmolb.2022.871737/fullRNA modificationhypoxic stressGC-2spd cellsspermatogenesishigh-throughput sequencingmass spectrometry |
| spellingShingle | Tong He Tong He Huanping Guo Lin Xia Xipeng Shen Xipeng Shen Yun Huang Xiao Wu Xuelin Jiang Yinying Xu Yi Tan Yunfang Zhang Yunfang Zhang Dongmei Tan Alterations of RNA Modification in Mouse Germ Cell-2 Spermatids Under Hypoxic Stress Frontiers in Molecular Biosciences RNA modification hypoxic stress GC-2spd cells spermatogenesis high-throughput sequencing mass spectrometry |
| title | Alterations of RNA Modification in Mouse Germ Cell-2 Spermatids Under Hypoxic Stress |
| title_full | Alterations of RNA Modification in Mouse Germ Cell-2 Spermatids Under Hypoxic Stress |
| title_fullStr | Alterations of RNA Modification in Mouse Germ Cell-2 Spermatids Under Hypoxic Stress |
| title_full_unstemmed | Alterations of RNA Modification in Mouse Germ Cell-2 Spermatids Under Hypoxic Stress |
| title_short | Alterations of RNA Modification in Mouse Germ Cell-2 Spermatids Under Hypoxic Stress |
| title_sort | alterations of rna modification in mouse germ cell 2 spermatids under hypoxic stress |
| topic | RNA modification hypoxic stress GC-2spd cells spermatogenesis high-throughput sequencing mass spectrometry |
| url | https://www.frontiersin.org/articles/10.3389/fmolb.2022.871737/full |
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