MicroRNA-300: A Transcellular Mediator in Exosome Regulates Melanoma Progression
Melanoma is a common and high-mortality skin cancer. Oxidative stress and DNA damage caused by ultraviolet light (UV) are major causative factors of melanoma formation. However, the specific molecular mechanism is still unclear. In this study, 218 dysregulated genes and 104 dysregulated miRNAs in re...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-10-01
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| Series: | Frontiers in Oncology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fonc.2019.01005/full |
| _version_ | 1819170258017058816 |
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| author | Long Chen Vega Windy Karisma Huawen Liu Li Zhong |
| author_facet | Long Chen Vega Windy Karisma Huawen Liu Li Zhong |
| author_sort | Long Chen |
| collection | DOAJ |
| description | Melanoma is a common and high-mortality skin cancer. Oxidative stress and DNA damage caused by ultraviolet light (UV) are major causative factors of melanoma formation. However, the specific molecular mechanism is still unclear. In this study, 218 dysregulated genes and 104 dysregulated miRNAs in response to UV were screened by analyzing sequencing datasets. Among them, 29 up-regulated miRNAs and 28 down-regulated miRNAs were involved in the melanoma pathway. As the only differential gene in the melanoma pathway, GADD45B severely affects the prognosis of melanoma patients. MiR-300 is the only differentially expressed miRNA that regulates GADD45B. In addition, compared to normal melanocytes, miR-300 was significantly down-regulated in melanoma cells (log FC = −1.63) and exosomes (log FC = −1.34). Among the transcription factors predicted to regulate miR-300, MYC, PPARG, and ZIC2 were significantly up-regulated in melanoma cells, and TP53, JUN, JUNB, FOS, and FOSB interacted with GADD45B. We attempted to reveal the pathogenesis of melanoma and screen new biomarkers by constructing a TF-mRNA-miRNA axis in turn to provide a view for further research. |
| first_indexed | 2024-12-22T19:32:32Z |
| format | Article |
| id | doaj.art-786f9b35758742b9a454bb14439b52fa |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-12-22T19:32:32Z |
| publishDate | 2019-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-786f9b35758742b9a454bb14439b52fa2022-12-21T18:15:04ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2019-10-01910.3389/fonc.2019.01005480624MicroRNA-300: A Transcellular Mediator in Exosome Regulates Melanoma ProgressionLong Chen0Vega Windy Karisma1Huawen Liu2Li Zhong3Bioengineering Institute of Chongqing University, Chongqing, ChinaBioengineering Institute of Chongqing University, Chongqing, ChinaThree Gorges Central Hospital, Chongqing, ChinaBioengineering Institute of Chongqing University, Chongqing, ChinaMelanoma is a common and high-mortality skin cancer. Oxidative stress and DNA damage caused by ultraviolet light (UV) are major causative factors of melanoma formation. However, the specific molecular mechanism is still unclear. In this study, 218 dysregulated genes and 104 dysregulated miRNAs in response to UV were screened by analyzing sequencing datasets. Among them, 29 up-regulated miRNAs and 28 down-regulated miRNAs were involved in the melanoma pathway. As the only differential gene in the melanoma pathway, GADD45B severely affects the prognosis of melanoma patients. MiR-300 is the only differentially expressed miRNA that regulates GADD45B. In addition, compared to normal melanocytes, miR-300 was significantly down-regulated in melanoma cells (log FC = −1.63) and exosomes (log FC = −1.34). Among the transcription factors predicted to regulate miR-300, MYC, PPARG, and ZIC2 were significantly up-regulated in melanoma cells, and TP53, JUN, JUNB, FOS, and FOSB interacted with GADD45B. We attempted to reveal the pathogenesis of melanoma and screen new biomarkers by constructing a TF-mRNA-miRNA axis in turn to provide a view for further research.https://www.frontiersin.org/article/10.3389/fonc.2019.01005/fullUVGadd45bmiR-300exosomemelanoma |
| spellingShingle | Long Chen Vega Windy Karisma Huawen Liu Li Zhong MicroRNA-300: A Transcellular Mediator in Exosome Regulates Melanoma Progression Frontiers in Oncology UV Gadd45b miR-300 exosome melanoma |
| title | MicroRNA-300: A Transcellular Mediator in Exosome Regulates Melanoma Progression |
| title_full | MicroRNA-300: A Transcellular Mediator in Exosome Regulates Melanoma Progression |
| title_fullStr | MicroRNA-300: A Transcellular Mediator in Exosome Regulates Melanoma Progression |
| title_full_unstemmed | MicroRNA-300: A Transcellular Mediator in Exosome Regulates Melanoma Progression |
| title_short | MicroRNA-300: A Transcellular Mediator in Exosome Regulates Melanoma Progression |
| title_sort | microrna 300 a transcellular mediator in exosome regulates melanoma progression |
| topic | UV Gadd45b miR-300 exosome melanoma |
| url | https://www.frontiersin.org/article/10.3389/fonc.2019.01005/full |
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