Sperm Parameters and Chromatin Integrity in Men Suffering from Celiac Disease: Insights into Reproductive Health, Case-Control Study
Objective: Celiac disease is a common chronic inflammatory condition of the small intestine caused by permanentintolerance to gluten/gliadin. It has been demonstrated that oxidative stress is one of the mechanisms that is involvedin gliadin toxicity, and there is a correlation between oxidative dama...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Royan Institute (ACECR), Tehran
2024-03-01
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| Series: | Cell Journal |
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| Online Access: | https://www.celljournal.org/article_711797_5c4771407a11b8ecd2805796b92c86b1.pdf |
| _version_ | 1797205792555794432 |
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| author | Shaghayegh Kiani Marziyeh Tavalaee Fatemeh Maghool Nahid Jamali Mohammad Hassan Emami Mohammad Hossein Nasr-Esfahani |
| author_facet | Shaghayegh Kiani Marziyeh Tavalaee Fatemeh Maghool Nahid Jamali Mohammad Hassan Emami Mohammad Hossein Nasr-Esfahani |
| author_sort | Shaghayegh Kiani |
| collection | DOAJ |
| description | Objective: Celiac disease is a common chronic inflammatory condition of the small intestine caused by permanentintolerance to gluten/gliadin. It has been demonstrated that oxidative stress is one of the mechanisms that is involvedin gliadin toxicity, and there is a correlation between oxidative damage with this disease. Similarly, increased oxidativestress was repeatedly reported in infertile men which led to low-quality of sperm function. Therefore, we aimed toassess sperm parameters and chromatin status in men with Celiac disease.Materials and Methods: In this case-control study, semen samples were collected from 11 fertile men without Celiacand 10 men with diagnostic Celiac disease. Basic semen analyses were performed according to the World HealthOrganization (WHO) 2010 protocol. The percentage of sperm with persistence histones, protamine deficiency, DNAfragmentation, malondialdehyde (MDA), and intracellular reactive oxygen species (ROS) were assessed using anilineblue, chromomycin A3, sperm chromatin structure assay, thiobarbituric acid reactive substances (TBARS) assay, anddiacetyldichlorofluorescein staining, respectively.Results: Unlike the sperm parameters, which did not show significant differences between men with Celiac diseaseand fertile individuals, sperm chromatin maturation (persistence histones and protamine deficiency) and sperm DNAdamage in men with Celiac disease were significantly higher compared to fertile individuals (P<0.05). In addition, thepercentage of sperm viability in these individuals was significantly lower than that in the fertile individuals (P<0.05). Wedid not observe any significant differences in sperm lipid peroxidation and intracellular ROS levels between the twostudy groups (P>0.05).Conclusion: Celiac disease affects sperm chromatin maturation and DNA fragmentation, emphasizing its impact onreproductive health. |
| first_indexed | 2024-04-24T08:56:45Z |
| format | Article |
| id | doaj.art-786fae63c46d4c91875f9d848ffc5aaa |
| institution | Directory Open Access Journal |
| issn | 2228-5806 2228-5814 |
| language | English |
| last_indexed | 2024-04-24T08:56:45Z |
| publishDate | 2024-03-01 |
| publisher | Royan Institute (ACECR), Tehran |
| record_format | Article |
| series | Cell Journal |
| spelling | doaj.art-786fae63c46d4c91875f9d848ffc5aaa2024-04-16T07:17:42ZengRoyan Institute (ACECR), TehranCell Journal2228-58062228-58142024-03-0126320220810.22074/cellj.2024.2014048.1432711797Sperm Parameters and Chromatin Integrity in Men Suffering from Celiac Disease: Insights into Reproductive Health, Case-Control StudyShaghayegh Kiani0Marziyeh Tavalaee1Fatemeh Maghool2Nahid Jamali3Mohammad Hassan Emami4Mohammad Hossein Nasr-Esfahani5Department of Animal Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, IranDepartment of Animal Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, IranPoursina Hakim Digestive Diseases Research Center, Isfahan University of Medical Sciences, Isfahan, IranPoursina Hakim Digestive Diseases Research Center, Isfahan University of Medical Sciences, Isfahan, IranPoursina Hakim Digestive Diseases Research Center, Isfahan University of Medical Sciences, Isfahan, IranDepartment of Animal Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, IranObjective: Celiac disease is a common chronic inflammatory condition of the small intestine caused by permanentintolerance to gluten/gliadin. It has been demonstrated that oxidative stress is one of the mechanisms that is involvedin gliadin toxicity, and there is a correlation between oxidative damage with this disease. Similarly, increased oxidativestress was repeatedly reported in infertile men which led to low-quality of sperm function. Therefore, we aimed toassess sperm parameters and chromatin status in men with Celiac disease.Materials and Methods: In this case-control study, semen samples were collected from 11 fertile men without Celiacand 10 men with diagnostic Celiac disease. Basic semen analyses were performed according to the World HealthOrganization (WHO) 2010 protocol. The percentage of sperm with persistence histones, protamine deficiency, DNAfragmentation, malondialdehyde (MDA), and intracellular reactive oxygen species (ROS) were assessed using anilineblue, chromomycin A3, sperm chromatin structure assay, thiobarbituric acid reactive substances (TBARS) assay, anddiacetyldichlorofluorescein staining, respectively.Results: Unlike the sperm parameters, which did not show significant differences between men with Celiac diseaseand fertile individuals, sperm chromatin maturation (persistence histones and protamine deficiency) and sperm DNAdamage in men with Celiac disease were significantly higher compared to fertile individuals (P<0.05). In addition, thepercentage of sperm viability in these individuals was significantly lower than that in the fertile individuals (P<0.05). Wedid not observe any significant differences in sperm lipid peroxidation and intracellular ROS levels between the twostudy groups (P>0.05).Conclusion: Celiac disease affects sperm chromatin maturation and DNA fragmentation, emphasizing its impact onreproductive health.https://www.celljournal.org/article_711797_5c4771407a11b8ecd2805796b92c86b1.pdfceliacchromatinoxidative stresssperm parameters |
| spellingShingle | Shaghayegh Kiani Marziyeh Tavalaee Fatemeh Maghool Nahid Jamali Mohammad Hassan Emami Mohammad Hossein Nasr-Esfahani Sperm Parameters and Chromatin Integrity in Men Suffering from Celiac Disease: Insights into Reproductive Health, Case-Control Study Cell Journal celiac chromatin oxidative stress sperm parameters |
| title | Sperm Parameters and Chromatin Integrity in Men Suffering from Celiac Disease: Insights into Reproductive Health, Case-Control Study |
| title_full | Sperm Parameters and Chromatin Integrity in Men Suffering from Celiac Disease: Insights into Reproductive Health, Case-Control Study |
| title_fullStr | Sperm Parameters and Chromatin Integrity in Men Suffering from Celiac Disease: Insights into Reproductive Health, Case-Control Study |
| title_full_unstemmed | Sperm Parameters and Chromatin Integrity in Men Suffering from Celiac Disease: Insights into Reproductive Health, Case-Control Study |
| title_short | Sperm Parameters and Chromatin Integrity in Men Suffering from Celiac Disease: Insights into Reproductive Health, Case-Control Study |
| title_sort | sperm parameters and chromatin integrity in men suffering from celiac disease insights into reproductive health case control study |
| topic | celiac chromatin oxidative stress sperm parameters |
| url | https://www.celljournal.org/article_711797_5c4771407a11b8ecd2805796b92c86b1.pdf |
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