Response of human normal and leukemia cells to factors released by amnion fragments in vitro.
Amnion is a membrane surrounding the embryo/fetus which determine growth factors and interleukins with angiogenic, immunogenic, and anti-inflammatory properties. The aim of the present study was to investigate the effects of conditioned culture medium from 24-h cultures of human amnion (hAM CCM) on...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2018-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5875856?pdf=render |
| _version_ | 1811296263322206208 |
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| author | Zofia Grzywocz Grazyna Hoser Stanislawa Sabalinska Piotr Ladyzynski Jaroslaw Czubak Malgorzata Dworczynska Romuald Debski Ewa Pius-Sadowska Boguslaw Machalinski Jerzy Kawiak |
| author_facet | Zofia Grzywocz Grazyna Hoser Stanislawa Sabalinska Piotr Ladyzynski Jaroslaw Czubak Malgorzata Dworczynska Romuald Debski Ewa Pius-Sadowska Boguslaw Machalinski Jerzy Kawiak |
| author_sort | Zofia Grzywocz |
| collection | DOAJ |
| description | Amnion is a membrane surrounding the embryo/fetus which determine growth factors and interleukins with angiogenic, immunogenic, and anti-inflammatory properties. The aim of the present study was to investigate the effects of conditioned culture medium from 24-h cultures of human amnion (hAM CCM) on migration and proliferation of human umbilical vein endothelial primary cells (HUVECs), freshly isolated bone marrow mononuclear cells (BM MNCs), and Jurkat leukemia cell line. Amnion membrane was freshly isolated from healthy placenta and its fragments cultured in vitro to produce hAM CCM. Members of the IGFBP protein family made up one third of all assayed proteins present in the hAM medium. The hAM CCM did not affect the proliferation rate of HUVECs or MNCs, but we observed more intensive migration of those cells, and lower expression of CD31 surface antigen on HUVECs as compared to control cultures. In contrast, Jurkat cells did not respond to hAM CCM treatment by proliferation or mobility change. The conditioned medium from 24-h cultures of human amnion is easy to obtain and is a convenient source of various growth and other factors that may be useful in practical medicine. |
| first_indexed | 2024-04-13T05:46:01Z |
| format | Article |
| id | doaj.art-78705fe8d6594db5b22a876d4ff2ae5b |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-13T05:46:01Z |
| publishDate | 2018-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-78705fe8d6594db5b22a876d4ff2ae5b2022-12-22T02:59:57ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-01133e019503510.1371/journal.pone.0195035Response of human normal and leukemia cells to factors released by amnion fragments in vitro.Zofia GrzywoczGrazyna HoserStanislawa SabalinskaPiotr LadyzynskiJaroslaw CzubakMalgorzata DworczynskaRomuald DebskiEwa Pius-SadowskaBoguslaw MachalinskiJerzy KawiakAmnion is a membrane surrounding the embryo/fetus which determine growth factors and interleukins with angiogenic, immunogenic, and anti-inflammatory properties. The aim of the present study was to investigate the effects of conditioned culture medium from 24-h cultures of human amnion (hAM CCM) on migration and proliferation of human umbilical vein endothelial primary cells (HUVECs), freshly isolated bone marrow mononuclear cells (BM MNCs), and Jurkat leukemia cell line. Amnion membrane was freshly isolated from healthy placenta and its fragments cultured in vitro to produce hAM CCM. Members of the IGFBP protein family made up one third of all assayed proteins present in the hAM medium. The hAM CCM did not affect the proliferation rate of HUVECs or MNCs, but we observed more intensive migration of those cells, and lower expression of CD31 surface antigen on HUVECs as compared to control cultures. In contrast, Jurkat cells did not respond to hAM CCM treatment by proliferation or mobility change. The conditioned medium from 24-h cultures of human amnion is easy to obtain and is a convenient source of various growth and other factors that may be useful in practical medicine.http://europepmc.org/articles/PMC5875856?pdf=render |
| spellingShingle | Zofia Grzywocz Grazyna Hoser Stanislawa Sabalinska Piotr Ladyzynski Jaroslaw Czubak Malgorzata Dworczynska Romuald Debski Ewa Pius-Sadowska Boguslaw Machalinski Jerzy Kawiak Response of human normal and leukemia cells to factors released by amnion fragments in vitro. PLoS ONE |
| title | Response of human normal and leukemia cells to factors released by amnion fragments in vitro. |
| title_full | Response of human normal and leukemia cells to factors released by amnion fragments in vitro. |
| title_fullStr | Response of human normal and leukemia cells to factors released by amnion fragments in vitro. |
| title_full_unstemmed | Response of human normal and leukemia cells to factors released by amnion fragments in vitro. |
| title_short | Response of human normal and leukemia cells to factors released by amnion fragments in vitro. |
| title_sort | response of human normal and leukemia cells to factors released by amnion fragments in vitro |
| url | http://europepmc.org/articles/PMC5875856?pdf=render |
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