3D Cell Cultures as Prospective Models to Study Extracellular Vesicles in Cancer
The improvement of culturing techniques to model the environment and physiological conditions surrounding tumors has also been applied to the study of extracellular vesicles (EVs) in cancer research. EVs role is not only limited to cell-to-cell communication in tumor physiology, they are also a prom...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-01-01
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| Series: | Cancers |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-6694/13/2/307 |
| _version_ | 1797411031070277632 |
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| author | Guillermo Bordanaba-Florit Iratxe Madarieta Beatriz Olalde Juan M. Falcón-Pérez Félix Royo |
| author_facet | Guillermo Bordanaba-Florit Iratxe Madarieta Beatriz Olalde Juan M. Falcón-Pérez Félix Royo |
| author_sort | Guillermo Bordanaba-Florit |
| collection | DOAJ |
| description | The improvement of culturing techniques to model the environment and physiological conditions surrounding tumors has also been applied to the study of extracellular vesicles (EVs) in cancer research. EVs role is not only limited to cell-to-cell communication in tumor physiology, they are also a promising source of biomarkers, and a tool to deliver drugs and induce antitumoral activity. In the present review, we have addressed the improvements achieved by using 3D culture models to evaluate the role of EVs in tumor progression and the potential applications of EVs in diagnostics and therapeutics. The most employed assays are gel-based spheroids, often utilized to examine the cell invasion rate and angiogenesis markers upon EVs treatment. To study EVs as drug carriers, a more complex multicellular cultures and organoids from cancer stem cell populations have been developed. Such strategies provide a closer response to in vivo physiology observed responses. They are also the best models to understand the complex interactions between different populations of cells and the extracellular matrix, in which tumor-derived EVs modify epithelial or mesenchymal cells to become protumor agents. Finally, the growth of cells in 3D bioreactor-like systems is appointed as the best approach to industrial EVs production, a necessary step toward clinical translation of EVs-based therapy. |
| first_indexed | 2024-03-09T04:39:05Z |
| format | Article |
| id | doaj.art-78725dd73b6a4f688dbe54e2b7c9408e |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-09T04:39:05Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-78725dd73b6a4f688dbe54e2b7c9408e2023-12-03T13:23:24ZengMDPI AGCancers2072-66942021-01-0113230710.3390/cancers130203073D Cell Cultures as Prospective Models to Study Extracellular Vesicles in CancerGuillermo Bordanaba-Florit0Iratxe Madarieta1Beatriz Olalde2Juan M. Falcón-Pérez3Félix Royo4Center for Cooperative Research in Biosciences (CIC bioGUNE), Exosomes Laboratory, Basque Research and Technology Alliance (BRTA), E48160 Derio, SpainTECNALIA Basque Research and Technology Alliance (BRTA), E20009 Donostia San Sebastian, SpainTECNALIA Basque Research and Technology Alliance (BRTA), E20009 Donostia San Sebastian, SpainCenter for Cooperative Research in Biosciences (CIC bioGUNE), Exosomes Laboratory, Basque Research and Technology Alliance (BRTA), E48160 Derio, SpainCenter for Cooperative Research in Biosciences (CIC bioGUNE), Exosomes Laboratory, Basque Research and Technology Alliance (BRTA), E48160 Derio, SpainThe improvement of culturing techniques to model the environment and physiological conditions surrounding tumors has also been applied to the study of extracellular vesicles (EVs) in cancer research. EVs role is not only limited to cell-to-cell communication in tumor physiology, they are also a promising source of biomarkers, and a tool to deliver drugs and induce antitumoral activity. In the present review, we have addressed the improvements achieved by using 3D culture models to evaluate the role of EVs in tumor progression and the potential applications of EVs in diagnostics and therapeutics. The most employed assays are gel-based spheroids, often utilized to examine the cell invasion rate and angiogenesis markers upon EVs treatment. To study EVs as drug carriers, a more complex multicellular cultures and organoids from cancer stem cell populations have been developed. Such strategies provide a closer response to in vivo physiology observed responses. They are also the best models to understand the complex interactions between different populations of cells and the extracellular matrix, in which tumor-derived EVs modify epithelial or mesenchymal cells to become protumor agents. Finally, the growth of cells in 3D bioreactor-like systems is appointed as the best approach to industrial EVs production, a necessary step toward clinical translation of EVs-based therapy.https://www.mdpi.com/2072-6694/13/2/3073D cultureextracellular vesiclestumoral cellscancertherapy |
| spellingShingle | Guillermo Bordanaba-Florit Iratxe Madarieta Beatriz Olalde Juan M. Falcón-Pérez Félix Royo 3D Cell Cultures as Prospective Models to Study Extracellular Vesicles in Cancer Cancers 3D culture extracellular vesicles tumoral cells cancer therapy |
| title | 3D Cell Cultures as Prospective Models to Study Extracellular Vesicles in Cancer |
| title_full | 3D Cell Cultures as Prospective Models to Study Extracellular Vesicles in Cancer |
| title_fullStr | 3D Cell Cultures as Prospective Models to Study Extracellular Vesicles in Cancer |
| title_full_unstemmed | 3D Cell Cultures as Prospective Models to Study Extracellular Vesicles in Cancer |
| title_short | 3D Cell Cultures as Prospective Models to Study Extracellular Vesicles in Cancer |
| title_sort | 3d cell cultures as prospective models to study extracellular vesicles in cancer |
| topic | 3D culture extracellular vesicles tumoral cells cancer therapy |
| url | https://www.mdpi.com/2072-6694/13/2/307 |
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