Expression of Retroelements in Cervical Cancer and Their Interplay with HPV Infection and Host Gene Expression
Retroelements are expressed in diverse types of cancer and are related to tumorigenesis and to cancer progression. We characterized the expression of retroelements in cervical cancer and explored their interplay with HPV infection and their association with expression of neighboring genes. Forty bio...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-07-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/13/14/3513 |
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| author | Gislaine Curty Albert N. Menezes Ayslan C. Brant Miguel de Mulder Rougvie Miguel Ângelo M. Moreira Marcelo A. Soares |
| author_facet | Gislaine Curty Albert N. Menezes Ayslan C. Brant Miguel de Mulder Rougvie Miguel Ângelo M. Moreira Marcelo A. Soares |
| author_sort | Gislaine Curty |
| collection | DOAJ |
| description | Retroelements are expressed in diverse types of cancer and are related to tumorigenesis and to cancer progression. We characterized the expression of retroelements in cervical cancer and explored their interplay with HPV infection and their association with expression of neighboring genes. Forty biopsies of invasive cervical carcinoma (squamous cell carcinomas and adenocarcinomas) with genotyped HPV were selected and analyzed for human endogenous retrovirus (HERV) and long interspersed nuclear element 1 (L1) expression through RNA-seq data. We found 8060 retroelements expressed in the samples and a negative correlation of DNA methyltransferase 1 expression with the two most expressed L1 elements. A total of 103 retroelements were found differentially expressed between tumor histological types and between HPV types, including several HERV families (HERV-K, HERV-H, HERV-E, HERV-I and HERV-L). The comparison between HPV mono- and co-infections showed the highest proportion of differentially expressed L1 elements. The location of retroelements affected neighboring gene expression, such as shown for the interleukin-20 gene family. Three HERVs and seven L1 were located close to this gene family and two L1 showed a positive association with <i>IL20RB</i> expression. This study describes the expression of retroelements in cervical cancer and shows their association with HPV status and host gene expression. |
| first_indexed | 2024-03-10T09:44:06Z |
| format | Article |
| id | doaj.art-78728bf2137d4130b75682bb2d0850e8 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-10T09:44:06Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-78728bf2137d4130b75682bb2d0850e82023-11-22T03:24:32ZengMDPI AGCancers2072-66942021-07-011314351310.3390/cancers13143513Expression of Retroelements in Cervical Cancer and Their Interplay with HPV Infection and Host Gene ExpressionGislaine Curty0Albert N. Menezes1Ayslan C. Brant2Miguel de Mulder Rougvie3Miguel Ângelo M. Moreira4Marcelo A. Soares5Oncovirology Program, Instituto Nacional de Câncer (INCA), Rio de Janeiro 20231-050, BrazilInstitute of Cancer and Genomic Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham B15 2TT, UKGenetics Program, Instituto Nacional de Câncer (INCA), Rio de Janeiro 20231-050, BrazilDivision of Infectious Diseases, Department of Medicine, Weill Cornell Medicine, New York, NY 10065, USAGenetics Program, Instituto Nacional de Câncer (INCA), Rio de Janeiro 20231-050, BrazilOncovirology Program, Instituto Nacional de Câncer (INCA), Rio de Janeiro 20231-050, BrazilRetroelements are expressed in diverse types of cancer and are related to tumorigenesis and to cancer progression. We characterized the expression of retroelements in cervical cancer and explored their interplay with HPV infection and their association with expression of neighboring genes. Forty biopsies of invasive cervical carcinoma (squamous cell carcinomas and adenocarcinomas) with genotyped HPV were selected and analyzed for human endogenous retrovirus (HERV) and long interspersed nuclear element 1 (L1) expression through RNA-seq data. We found 8060 retroelements expressed in the samples and a negative correlation of DNA methyltransferase 1 expression with the two most expressed L1 elements. A total of 103 retroelements were found differentially expressed between tumor histological types and between HPV types, including several HERV families (HERV-K, HERV-H, HERV-E, HERV-I and HERV-L). The comparison between HPV mono- and co-infections showed the highest proportion of differentially expressed L1 elements. The location of retroelements affected neighboring gene expression, such as shown for the interleukin-20 gene family. Three HERVs and seven L1 were located close to this gene family and two L1 showed a positive association with <i>IL20RB</i> expression. This study describes the expression of retroelements in cervical cancer and shows their association with HPV status and host gene expression.https://www.mdpi.com/2072-6694/13/14/3513retroelementsHERVL1LINE-1cervical cancerHPV |
| spellingShingle | Gislaine Curty Albert N. Menezes Ayslan C. Brant Miguel de Mulder Rougvie Miguel Ângelo M. Moreira Marcelo A. Soares Expression of Retroelements in Cervical Cancer and Their Interplay with HPV Infection and Host Gene Expression Cancers retroelements HERV L1 LINE-1 cervical cancer HPV |
| title | Expression of Retroelements in Cervical Cancer and Their Interplay with HPV Infection and Host Gene Expression |
| title_full | Expression of Retroelements in Cervical Cancer and Their Interplay with HPV Infection and Host Gene Expression |
| title_fullStr | Expression of Retroelements in Cervical Cancer and Their Interplay with HPV Infection and Host Gene Expression |
| title_full_unstemmed | Expression of Retroelements in Cervical Cancer and Their Interplay with HPV Infection and Host Gene Expression |
| title_short | Expression of Retroelements in Cervical Cancer and Their Interplay with HPV Infection and Host Gene Expression |
| title_sort | expression of retroelements in cervical cancer and their interplay with hpv infection and host gene expression |
| topic | retroelements HERV L1 LINE-1 cervical cancer HPV |
| url | https://www.mdpi.com/2072-6694/13/14/3513 |
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