Ex vivo characterization of Breg cells in patients with chronic Chagas disease
Abstract Despite the growing importance of the regulatory function of B cells in many infectious diseases, their immunosuppressive role remains elusive in chronic Chagas disease (CCD). Here, we studied the proportion of different B cell subsets and their capacity to secrete IL-10 ex vivo in peripher...
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| Format: | Article |
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Nature Portfolio
2021-03-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-84765-x |
| _version_ | 1818735285624635392 |
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| author | Magalí C. Girard Gonzalo R. Acevedo Micaela S. Ossowski Marisa Fernández Yolanda Hernández Raúl Chadi Karina A. Gómez |
| author_facet | Magalí C. Girard Gonzalo R. Acevedo Micaela S. Ossowski Marisa Fernández Yolanda Hernández Raúl Chadi Karina A. Gómez |
| author_sort | Magalí C. Girard |
| collection | DOAJ |
| description | Abstract Despite the growing importance of the regulatory function of B cells in many infectious diseases, their immunosuppressive role remains elusive in chronic Chagas disease (CCD). Here, we studied the proportion of different B cell subsets and their capacity to secrete IL-10 ex vivo in peripheral blood from patients with or without CCD cardiomyopathy. First, we immunophenotyped peripheral blood mononuclear cells from patients according to the expression of markers CD19, CD24, CD38 and CD27 and we showed an expansion of total B cell and transitional CD24highCD38high B cell subsets in CCD patients with cardiac involvement compared to non-infected donors. Although no differences were observed in the frequency of total IL-10 producing B cells (B10) among the groups, CCD patients with cardiac involvement showed an increased proportion of naïve B10 cells and a tendency to a higher frequency of transitional B10 cells compared to non-infected donors. Our research demonstrates that transitional B cells are greatly expanded in patients with the cardiac form of CCD and these cells retain the ability to secrete IL-10. These findings provide insight into the phenotypic distribution of regulatory B cells in CCD, an important step towards new strategies to prevent cardiomyopathy associated with T. cruzi infection. |
| first_indexed | 2024-12-18T00:18:50Z |
| format | Article |
| id | doaj.art-787794689dc04613a2f78dfff3a211c1 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-18T00:18:50Z |
| publishDate | 2021-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-787794689dc04613a2f78dfff3a211c12022-12-21T21:27:24ZengNature PortfolioScientific Reports2045-23222021-03-0111111510.1038/s41598-021-84765-xEx vivo characterization of Breg cells in patients with chronic Chagas diseaseMagalí C. Girard0Gonzalo R. Acevedo1Micaela S. Ossowski2Marisa Fernández3Yolanda Hernández4Raúl Chadi5Karina A. Gómez6Laboratorio de Inmunología de las Infecciones por Tripanosomátidos, Instituto de Investigaciones en Ingeniería Genética y Biología Molecular “Dr. Hector N. Torres” (INGEBI-CONICET)Laboratorio de Inmunología de las Infecciones por Tripanosomátidos, Instituto de Investigaciones en Ingeniería Genética y Biología Molecular “Dr. Hector N. Torres” (INGEBI-CONICET)Laboratorio de Inmunología de las Infecciones por Tripanosomátidos, Instituto de Investigaciones en Ingeniería Genética y Biología Molecular “Dr. Hector N. Torres” (INGEBI-CONICET)Instituto Nacional de Parasitología “Dr. Mario Fatala Chabén”Instituto Nacional de Parasitología “Dr. Mario Fatala Chabén”Hospital Nacional de Agudos “Dr. Ignacio Pirovano”Laboratorio de Inmunología de las Infecciones por Tripanosomátidos, Instituto de Investigaciones en Ingeniería Genética y Biología Molecular “Dr. Hector N. Torres” (INGEBI-CONICET)Abstract Despite the growing importance of the regulatory function of B cells in many infectious diseases, their immunosuppressive role remains elusive in chronic Chagas disease (CCD). Here, we studied the proportion of different B cell subsets and their capacity to secrete IL-10 ex vivo in peripheral blood from patients with or without CCD cardiomyopathy. First, we immunophenotyped peripheral blood mononuclear cells from patients according to the expression of markers CD19, CD24, CD38 and CD27 and we showed an expansion of total B cell and transitional CD24highCD38high B cell subsets in CCD patients with cardiac involvement compared to non-infected donors. Although no differences were observed in the frequency of total IL-10 producing B cells (B10) among the groups, CCD patients with cardiac involvement showed an increased proportion of naïve B10 cells and a tendency to a higher frequency of transitional B10 cells compared to non-infected donors. Our research demonstrates that transitional B cells are greatly expanded in patients with the cardiac form of CCD and these cells retain the ability to secrete IL-10. These findings provide insight into the phenotypic distribution of regulatory B cells in CCD, an important step towards new strategies to prevent cardiomyopathy associated with T. cruzi infection.https://doi.org/10.1038/s41598-021-84765-x |
| spellingShingle | Magalí C. Girard Gonzalo R. Acevedo Micaela S. Ossowski Marisa Fernández Yolanda Hernández Raúl Chadi Karina A. Gómez Ex vivo characterization of Breg cells in patients with chronic Chagas disease Scientific Reports |
| title | Ex vivo characterization of Breg cells in patients with chronic Chagas disease |
| title_full | Ex vivo characterization of Breg cells in patients with chronic Chagas disease |
| title_fullStr | Ex vivo characterization of Breg cells in patients with chronic Chagas disease |
| title_full_unstemmed | Ex vivo characterization of Breg cells in patients with chronic Chagas disease |
| title_short | Ex vivo characterization of Breg cells in patients with chronic Chagas disease |
| title_sort | ex vivo characterization of breg cells in patients with chronic chagas disease |
| url | https://doi.org/10.1038/s41598-021-84765-x |
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